Modelling cortical cataractogenesis XVIII. In vitro diabetic cataract reduction by venoruton

Abstract

The effect of a novel flavonoid, venoruton (a mixture of mono-, di-, tri- and tetrahydroxyethylrutosides) has been investigated in healthy rat lenses and compared with diabetic cataract modelled in vitro. One mM venoruton was added to medium simulating healthy and diabetic conditions for the incubated lenses; damage was followed by either stereoscopic photography of the lenses under a Cooperative Cataract Research Group operating microscope or with our recently developed method: the leakage of lactate dehydrogenase (LDH) into the lens culture media. The increased LDH activity in the medium and observable development of the opacity were correlated with cell damage, which has been found to be associated with globular degeneration and cataract formation. The extent of opacification and LDH release is reduced if 1 mM venoruton is included in the medium. The protective effect may be related to antioxidant activity against reactive oxygen species: decreased luminol luminescence was shown after venoruton addition to either superoxide-generating hypoxanthine plus xanthine oxidase, or hydrogen peroxide.

Efficacy of Troxerutin on Streptozotocin-induced Rat Model in the Early Stage of Diabetic Retinopathy

“…On the other hand, Vaccinium myrtillus and calcium dobesilate had little effect on the VEGF level despite their antioxidant properties. In addition, Kilic et al [33] reported that the flavonoid preparation Venoruton (a mixture of mono-, di-, tri-and tetrahydroxyethylrutoside), which prevents lens opacification, reduced the level of diabetic cataracts in vitro as a result of its antioxidant effect. However, troxerutin was not effective in preventing cataracts in this study [33].…”

“…In addition, Kilic et al [33] reported that the flavonoid preparation Venoruton (a mixture of mono-, di-, tri-and tetrahydroxyethylrutoside), which prevents lens opacification, reduced the level of diabetic cataracts in vitro as a result of its antioxidant effect. However, troxerutin was not effective in preventing cataracts in this study [33]. Also, no dose-effect relationship for troxerutin on body weight and blood glucose was observed in our study or in previous studies.…”

Fundamentals of Space Medicine

“…Vitamin E, vitamin C, and betacarotene are well-known and effective antioxidants. Novel and new flavanoids, such as venoruton, have also been shown to decrease cataract formation in rat models [Kilic et al, 1996].…”

Preventive Effect ofZea maysL. (Purple Waxy Corn) on Experimental Diabetic Cataract

“…The decrease of antioxidant enzymes mentioned earlier appears to play a pivotal role on the elevation of oxidative stress and cataractogenesis of diabetic cataract [5]. Several lines of evidence have demonstrated that flavonoids, the important phenolic compounds in fruits and vegetables, exert the protective effect against cataractogenesis of diabetic cataract [6, 7]. …”