Modelling and simulation of cell growth dynamics, substrate consumption, and lactic acid production kinetics of Lactococcus lactis

Zacharof, Myrto-Panagiota; Lovitt, Robert W.

doi:10.1007/s12257-012-0477-4

Cited by 31 publications

(21 citation statements)

References 49 publications

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“…The bacterium was revived twice by inoculating the selected strain into 50 ml serum vials containing optimised liquid medium (yeast extract 10 g L -1 , glucose 10 g L -1 , ammonium sulphate 5 g L -1 and 2.5 g L -1 potassium dihydrogen orthophosphate) and were statically incubated at 37 °C (Thermo Scientific Series 6000 Incubator, USA) for 24 h. Stock culture solutions of each strain were made through cryopreservation method [22,23] . For constant use, the bacterium was regularly inoculated (on a weekly basis) into 30 ml serum vials containing standard medium and were preserved at 2 °C [24].…”

Section: Preservation Of Microorganismmentioning

confidence: 99%

Intensive Production of Carboxylic Acids Using <em>Cl. butyricum</em> in a Membrane Bioreactor (MBR)

Tajarudin¹,

Zacharof²,

Ratanapongleka³

et al. 2018

Preprint

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This work reports of the use of a bench scale chemostat (CSTR) in continuous mode and of a pilot scale membrane bioreactor (MBR) in fed-batch mode to intensively produce acetic and butyric acids using Cl. butyricum grown on synthetic media. These studies were then used to perform a cost estimation study of the MBR system to assess the potential economic impact of this proposed methodology. The MBR system was found highly productive reaching 37.88 g L -1 h-1 of acetic and 14.44 g L -1 h -1 of volumetric cell productivity, favoring acetic acid production to butyric at a ratio 3:1. The cost of preparation and production of carboxylic acids using this system was found as 0.0062 £PS/kg respectively and an up to 99% carbon recovery.

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Section: Preservation Of Microorganismmentioning

confidence: 99%

Intensive Production of Carboxylic Acids Using <em>Cl. butyricum</em> in a Membrane Bioreactor (MBR)

Tajarudin¹,

Zacharof²,

Ratanapongleka³

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…The bacterium was revived twice by inoculating the selected strain into 50 ml serum vials containing basal medium (Luria Bertani broth, LB) and were statically incubated at 37°C (Thermo Scientific Series 6000 Incubator, USA) for 24 hours. Stock culture solutions of each strain were made through cryopreservation method [11]. For constant use, the bacterium was regularly reinoculated (on a weekly basis) into 30 ml serum vials containing basal medium and were preserved at 2°C [11].…”

Section: Bacterial Strainmentioning

confidence: 99%

“…Stock culture solutions of each strain were made through cryopreservation method [11]. For constant use, the bacterium was regularly reinoculated (on a weekly basis) into 30 ml serum vials containing basal medium and were preserved at 2°C [11].…”

Section: Bacterial Strainmentioning

confidence: 99%

“…The same process was followed for the preparation of minimised media (tap water mixed with 2% w/v glucose in a 1:1 ratio), treated agricultural wastewater and enriched agricultural with LB broth and 2% w/v glucose solution added in a 1:1 ratio. The flasks were gently mixed in a vortex and inoculated with 10 ml inoculum size, and incubated in a rotary shaker at 37 °C (Thermo Scientific Incubator, UK) at 37°C [11].…”

Section: Growth On Lb Broth Minimised Media and Treated Agriculturalmentioning

confidence: 99%

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The use of membrane technology for the formulation of spent anaerobic digester effluents as a nutrient source for bacterial growth

Zacharof¹,

Vouzelaud²,

Lovitt³

2014

WIT Transactions on Ecology and the Environment

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Spent digester effluents were formulated into nutrient media , suitable for microbial growth of industrially important microorganisms, using filtration and diafiltration. The waste effluents were pretreated by dilution, sedimentation and sieving for the removal of large particulate material. The scheme used allowed the successful formulation particle, free sterile effluents, with an N:P ratio 36.6 The prepared microfiltered effluents were then physicochemically characterized before being assessed for their utilization as growth substrates of microorganism associated with chemical and fuels production. Microfiltered treated digested propagated with Escherichia coli NCIMB 8277, agricultural sludge based media were compared with in vitro media when supplied with or without addition carbohydrate sources. in bench scale batch aerobic. Good growth was achieved compared to synthetic growth media with the microorganism using filtrates. When the treated effluents were supplied with 2% w/v glucose solution a significant improvement in the growth rates and growth yields was achieved giving comparable performance with the synthetic media.

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“…Thus, Zacharof and Lovitt [34] suggested the use of yield coefficients for both, substrate and production rates and furthermore, introduced a term for product inhibition into the specific growth rate. This model also considers microbial maintenance.…”

Section: Introductionmentioning

confidence: 99%

Experimental Investigation and Reaction Kinetics Modeling of Biomass Formation, Substrate Consumption and Product Formation During Startup of Fixed-Bed Cultures with Immobilized Lactococcus lactis ssp. lactis

Faschian¹,

Minden²,

Pörtner³

2016

J Bioprocess Biotech

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Modelling and simulation of cell growth dynamics, substrate consumption, and lactic acid production kinetics of Lactococcus lactis

Cited by 31 publications

References 49 publications

Intensive Production of Carboxylic Acids Using <em>Cl. butyricum</em> in a Membrane Bioreactor (MBR)

Intensive Production of Carboxylic Acids Using <em>Cl. butyricum</em> in a Membrane Bioreactor (MBR)

The use of membrane technology for the formulation of spent anaerobic digester effluents as a nutrient source for bacterial growth

Experimental Investigation and Reaction Kinetics Modeling of Biomass Formation, Substrate Consumption and Product Formation During Startup of Fixed-Bed Cultures with Immobilized Lactococcus lactis ssp. lactis

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