Modeling withdrawal syndrome in zebrafish

Cachat, Jonathan; Canavello, Peter R.; Elegante, Marco; Bartels, Brett; Hart, Peter C.; Bergner, Carisa L.; Egan, Rupert J.; Duncan, Ashley; Tien, David; Chung, Amanda; Wong, Keith; Goodspeed, Jason; Tan, Julia; Grimes, Chelsea; Elkhayat, Salem; Suciu, Christopher; Rosenberg, Michael; Chung, Kyung Min; Kadri, Ferdous; Roy, Sudipta; Gaikwad, Siddharth; Stewart, Adam; Zapolsky, Ivan; Gilder, Thomas; Mohnot, Sopan; Beeson, Esther; Amri, Hakima; Żukowska, Zofia; Soignier, R. Denis; Kalueff, Allan V.

doi:10.1016/j.bbr.2009.12.004

Cited by 160 publications

(126 citation statements)

References 68 publications

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“…Behavioral effects of drug withdrawal have been demonstrated with different drugs, including cocaine (López-Patiño, Yu, Cabral, et al, 2008;López-Patiño, Yu, Yamamoto, et al, 2008) and morphine (Cachat et al, 2010;Khor et al, 2011;Wong et al, 2010); in all cases, the behavioral endpoint assessed was anxiety-like behavior. In the same direction, ethanol withdrawal has been studied mainly with models for anxiety-like behavior (Table 3).…”

Section: Cc-by-ndmentioning

confidence: 99%

“…The inhibition of enzymatic activity observed here replicates what was observed by Müller et al (2017) in the zebrafish brain; in that study, however, the exposure duration was shorter (8 days), the concentration of EtOH was higher (1%), and the pattern of exposure was different (animals were exposed for 20 min per day, instead of continuously, to ethanol). The lack of effect on the head kidney, where interrenal cells (the teleost functional equivalent of the mammalian adrenal cortex) lie, suggest a brain-specific effect, and also suggests that possible effects on cortisol (e.g., Cachat et al, 2010) are not due to effects in these cells, but upstream in the hypothalamus-hypophyseal-interrenal axis.…”

Section: Cc-by-ndmentioning

confidence: 99%

“…Zebrafish anxiety-like behavior has been used to demonstrate the effects of drug withdrawal, including cocaine (López-Patiño, Yu, Cabral, & Zhdanova, 2008;López-Patiño, Yu, Yamamoto, & Zhdanova, 2008), morphine (Cachat et al, 2010;Khor, Jamil, Adenan, & Shu-Chien, 2011;Wong et al, 2010), and EtOH (S. Tran, Facciol, & Gerlai, 2016). In that last case, the literature is inconsistent, with some studies (e.g., Tran et al, 2015) reporting significant effects of withdrawal on anxiety-like behavior, while others (e. g., Cachat et al, 2010) were unable to detect effects of EtOH withdrawal.…”

Section: Introductionmentioning

confidence: 99%

“…In that last case, the literature is inconsistent, with some studies (e.g., Tran et al, 2015) reporting significant effects of withdrawal on anxiety-like behavior, while others (e. g., Cachat et al, 2010) were unable to detect effects of EtOH withdrawal. Procedural differences, such as assay type, strain, concentration during exposure, or withdrawal duration, could be responsible for this difference.…”

Section: Introductionmentioning

confidence: 99%

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Behavioral and biochemical effects of ethanol withdrawal in zebrafish

Chaves

Felicio

Costa

et al. 2017

Preprint

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Chronic alcohol use induces adaptations and toxicity that can induce symptoms of anxiety, autonomic hyperarousal, and seizures when alcohol is removed (withdrawal syndrome).Zebrafish has recently gained wide attention as a behavioral model to study the neurobehavioral effects of acute and chronic alcohol use, including withdrawal. The literature, however, is very contradictory on findings regarding withdrawal effects, with some studies reporting increased anxiety, while others report no effect. A meta-analytic approach was taken to find the sources of this heterogeneity, and ethanol concentration during exposure and exposure duration were found to be the main sources of variation. A conceptual replication was also made using continuous exposure for 16 days in waterborne ethanol (0.5%) and assessing anxiety-like behavior in the light/dark test after 60 min withdrawal. Withdrawal was shown to reduce preference for darkness, consistent with decreased anxiety, but to increase risk assessment, consistent with increased anxiety. Animals were also subjected to the withdrawal protocol and injected with pilocarpine in a sub-convulsive dose to assess susceptibility to seizures. The protocol was sufficient to increase susceptibility to seizures in animals exposed to ethanol. Finally, withdrawal also decreased catalase activity in the brain, but not in the head kidney, suggesting that oxidative stress could be a mechanism associated with the behavioral effects of ethanol withdrawal.

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Section: Cc-by-ndmentioning

confidence: 99%

Section: Cc-by-ndmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Behavioral and biochemical effects of ethanol withdrawal in zebrafish

Chaves

Felicio

Costa

et al. 2017

Preprint

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“…Both larval and adult zebrafish show high sensitivity to various drugs of abuse, as well as tolerance, clear preference (reward stimuli) for these agents, and withdrawal symptoms. 33,34 Rising evidence involves the zebrafish as a promising model species for reward and addiction research. Modeling drug abuse-related behaviour in both adult and larval zebrafish produced a wealth of clinically translatable data, also demonstrating their sensitivity to numerous drugs of abuse and the ability to develop tolerance.…”

Section: Drug Abuse and Withdrawalmentioning

confidence: 99%

Zebrafish: a novel model in neuropsychopharmacological research

Bhandari

2016

Int J Basic Clin Pharmacol

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Zebrafish embryos and larvae: A new generation of disease models and drug screens

Ali

Champagne

Spaink

et al. 2011

Birth Defects Research Part C: Embryo Today: Reviews

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127

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Technological innovation has helped the zebrafish embryo gain ground as a disease model and an assay system for drug screening. Here, we review the use of zebrafish embryos and early larvae in applied biomedical research, using selected cases. We look at the use of zebrafish embryos as disease models, taking fetal alcohol syndrome and tuberculosis as examples. We discuss advances in imaging, in culture techniques (including microfluidics), and in drug delivery (including new techniques for the robotic injection of compounds into the egg). The use of zebrafish embryos in early stages of drug safety-screening is discussed. So too are the new behavioral assays that are being adapted from rodent research for use in zebrafish embryos, and which may become relevant in validating the effects of neuroactive compounds such as anxiolytics and antidepressants. Readouts, such as morphological screening and cardiac function, are examined. There are several drawbacks in the zebrafish model. One is its very rapid development, which means that screening with zebrafish is analogous to "screening on a run-away train." Therefore, we argue that zebrafish embryos need to be precisely staged when used in acute assays, so as to ensure a consistent window of developmental exposure. We believe that zebrafish embryo screens can be used in the pre-regulatory phases of drug development, although more validation studies are needed to overcome industry scepticism. Finally, the zebrafish poses no challenge to the position of rodent models: it is complementary to them, especially in early stages of drug research.

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Modeling withdrawal syndrome in zebrafish

Cited by 160 publications

References 68 publications

Behavioral and biochemical effects of ethanol withdrawal in zebrafish

Behavioral and biochemical effects of ethanol withdrawal in zebrafish

Zebrafish: a novel model in neuropsychopharmacological research

Zebrafish embryos and larvae: A new generation of disease models and drug screens

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