Modeling ultrasound modulation of neural function in a single cell

Badawe, Heba M.; El Hassan, Rima H.; Khraiche, Massoud L.

doi:10.1016/j.heliyon.2023.e22522

Cited by 3 publications

(3 citation statements)

References 67 publications

(85 reference statements)

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“…This could be adopted due to the relatively small (∼ µm) dimensions of those spheres. Moreover, the cooling water was separated from the cell culture by a thin polymeric membrane that was disregarded in the computations since it is basically acoustically transparent [34,35].…”

Section: Computational Domainmentioning

confidence: 99%

Experimental and Computational Analysis of High-Intensity Focused Ultrasound Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

Badawe,

Harouz,

Raad

et al. 2024

Cancers

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High-intensity focused ultrasound (HIFU) is a non-invasive therapeutic modality that uses precise acoustic energy to ablate cancerous tissues through coagulative necrosis. In this context, we investigate the efficacy of HIFU ablation in two distinct cellular configurations, namely 2D monolayers and 3D spheroids of epithelial breast cancer cell lines (MDA-MB 231 and MCF7). The primary objective is to compare the response of these two in vitro models to HIFU while measuring their ablation percentages and temperature elevation levels. HIFU was systematically applied to the cell cultures, varying ultrasound intensity and duty cycle during different sonication sessions. The results indicate that the degree of ablation is highly influenced by the duty cycle, with higher duty cycles resulting in greater ablation percentages, while sonication duration has a minimal impact. Numerical simulations validate experimental observations, highlighting a significant disparity in the response of 2D monolayers and 3D spheroids to HIFU treatment. Specifically, tumor spheroids require lower temperature elevations for effective ablation, and their ablation percentage significantly increases with elevated duty cycles. This study contributes to a comprehensive understanding of acoustic energy conversion within the biological system during HIFU treatment for 2D versus 3D ablation targets, holding potential implications for refining and personalizing breast cancer therapeutic strategies.

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Section: Computational Domainmentioning

confidence: 99%

Experimental and Computational Analysis of High-Intensity Focused Ultrasound Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

Badawe,

Harouz,

Raad

et al. 2024

Cancers

Self Cite

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show abstract

“…Cancer poses a significant global public health challenge and currently ranks as the second leading cause of death worldwide, following cardiovascular diseases. Notably, among women, breast cancer emerges as the most extensively studied malignancy and a foremost contributor to cancerrelated fatalities (1,2). Traditional treatments for breast cancer, on the other hand, encompass a range of modalities contingent on factors such as cancer type, treatment tolerability, and disease stage.…”

Section: Introductionmentioning

confidence: 99%

“…HIFU can generate coagulative necrosis within a specific focal area within the body while sparing adjacent structures along the path of the acoustic beams (10,11). In general, HIFU induces two major biological effects on cell ablation: thermal and non-thermal (2,12,13). Thermal ablation results from the conversion of acoustic energy to thermal energy at the target site through an increase in the energy density, thus elevating the temperature of the cells to a range of 60-85 °C within seconds (14).…”

Section: Introductionmentioning

confidence: 99%

Experimental and Computational Analysis of HIFU Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

Badawe,

Harouz,

Abu

et al. 2023

Preprint

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ObjectiveThe primary objective of our study was to investigate the efficiency of high intensity focused ultrasound (HIFU) ablation in two distinct cellular configurations, 2D monolayers and 3D spheroids of epithelial breast cancer cell lines. The study also compares empirical findings from experiments with results obtained through numerical simulations using a bioheat computational model. This comparison is intended to provide a comprehensive understanding of the acoustic energy conversion within the biological system during HIFU treatment.MethodsHIFU was applied to 2D and 3D cultured MDA-MB 231 and MCF7 epithelial breast cancer cell lines while systematically varying ultrasound intensity and duty cycle (DC) during sonication sessions of different durations. Temperature elevation was measured and the ablation percentage was calculated based on bright field and fluorescent imaging of the treated regions. Experimental results were validated through simulations of the ablation setup.ResultsUpon HIFU, spheroids exhibited a lower temperature increase (approximately 20 °C) when subjected to comparable acoustic intensities and duty cycles. The level of tumor ablation was highly influenced by DC, with higher DCs leading to greater ablation percentages. However, sonication duration had a minimal impact on the degree of ablation. Numerical simulations corroborated these observations, demonstrating uniform heat distribution within the cultured cells. At higher DCs and intensities, complete ablation of spheroids was achieved, whereas at lower levels, only the outermost layers exhibited ablation.ConclusionOur study reveals a significant disparity in the response of 2D monolayers and 3D spheroids to HIFU treatment. Specifically, tumor spheroids require lower temperature elevations for effective ablation, and their ablation percentage significantly increases with elevated DC.

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Low frequency ultrasound elicits broad cortical responses inhibited by ketamine in mice

Shi,

Mastracchio,

Saytashev

et al. 2024

Commun Eng

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The neuromodulatory effects of >250 kHz ultrasound have been well-demonstrated, but the impact of lower-frequency ultrasound, which can transmit better through air and the skull, on the brain is unclear. This study investigates the biological impact of 40 kHz pulsed ultrasound on the brain using calcium imaging and electrophysiology in mice. Our findings reveal burst duration-dependent neural responses in somatosensory and auditory cortices, resembling responses to 12 kHz audible tone, in vivo. In vitro brain slice experiments show no neural responses to 300 kPa 40 kHz ultrasound, implying indirect network effects. Ketamine fully blocks neural responses to ultrasound in both cortices but only partially affects 12 kHz audible tone responses in the somatosensory cortex and has no impact on auditory cortex 12 kHz responses. This suggests that low-frequency ultrasound’s cortical effects rely heavily on NMDA receptors and may involve mechanisms beyond indirect auditory cortex activation. This research uncovers potential low-frequency ultrasound effects and mechanisms in the brain, offering a path for future neuromodulation.

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Modeling ultrasound modulation of neural function in a single cell

Cited by 3 publications

References 67 publications

Experimental and Computational Analysis of High-Intensity Focused Ultrasound Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

Experimental and Computational Analysis of High-Intensity Focused Ultrasound Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

Experimental and Computational Analysis of HIFU Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

Low frequency ultrasound elicits broad cortical responses inhibited by ketamine in mice

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