Modeling Tumor Phenotypes In Vitro with Three-Dimensional Bioprinting

Langer, Ellen M.; Allen-Petersen, Brittany L.; King, Shelby M.; Kendsersky, Nicholas D.; Turnidge, Megan A.; Kuziel, Genevra; Riggers, Rachelle; Samatham, Ravi; Amery, Taylor S.; Jacques, Steven L.; Sheppard, Brett C.; Korkola, James E.; Muschler, John; Thibault, Guillaume; Chang, Young Hwan; Gray, Joe W.; Presnell, Sharon C.; Nguyen, Deborah G.; Sears, Rosalie C.

doi:10.1016/j.celrep.2018.12.090

Cited by 186 publications

(172 citation statements)

References 68 publications

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“…3D-bioprinting models of breast and pancreatic cancer containing the stromal component (human umbilical vessel epithelial cells (HUVEC), fibroblasts, MSCs) and an ECM analog were described. The resulting 3D bioprinting models repeated the behavior of tumors in vivo and in situ (Langer et al, 2019).…”

Section: Bioprintingmentioning

confidence: 91%

Cell Culture Based in vitro Test Systems for Anticancer Drug Screening

Kitaeva

Rutland

Rizvanov

et al. 2020

Front. Bioeng. Biotechnol.

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The development of new high-tech systems for screening anticancer drugs is one of the main problems of preclinical screening. Poor correlation between preclinical in vitro and in vivo data with clinical trials remains a major concern. The choice of the correct tumor model at the stage of in vitro testing provides reduction in both financial and time costs during later stages due to the timely screening of ineffective agents. In view of the growing incidence of oncology, increasing the pace of the creation, development and testing of new antitumor agents, the improvement and expansion of new high-tech systems for preclinical in vitro screening is becoming very important. The pharmaceutical industry presently relies on several widely used in vitro models, including two-dimensional models, three-dimensional models, microfluidic systems, Boyden's chamber and models created using 3D bioprinting. This review outlines and describes these tumor models including their use in research, in addition to their characteristics. This review therefore gives an insight into in vitro based testing which is of interest to researchers and clinicians from differing fields including pharmacy, preclinical studies and cell biology.

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Section: Bioprintingmentioning

confidence: 91%

Cell Culture Based in vitro Test Systems for Anticancer Drug Screening

Kitaeva

Rutland

Rizvanov

et al. 2020

Front. Bioeng. Biotechnol.

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“…Vasculature created using this method had diameters of 400–1000 µm. Others have used 3D bioprinting to print tumor cells and ECs without accompanying ECM, with a result that is similar to an actual tumor with cell deposited ECM and organization of vasculature . Here, the bioink used included an alginate‐based hydrogel that could be tuned to provide a desired stiffness during cell seeding but could then be removed to leave a structure containing only cells.…”

Section: Methods To Vascularize Biomaterialsmentioning

confidence: 99%

Vascularized Biomaterials to Study Cancer Metastasis

Bittner

Jiménez

Peyton

2020

Adv Healthcare Materials

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Cancer metastasis, the spread of cancer cells to distant organs, is responsible for 90% of cancer‐related deaths. Cancer cells need to enter and exit circulation in order to form metastases, and the vasculature and endothelial cells are key regulators of this process. While vascularized 3D in vitro systems have been developed, few have been used to study cancer, and many lack key features of vessels that are necessary to study metastasis. This review focuses on current methods of vascularizing biomaterials for the study of cancer, and three main factors that regulate intravasation and extravasation: endothelial cell heterogeneity, hemodynamics, and the extracellular matrix of the perivascular niche.

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“…This technique allows the formation of complex tissues with a variety of cell types organized in a defined spatial architecture in a scaffold-free environment [294]. 3D bioprinted tumours in vitro can be used to test a variety of responses in tissues exposed to treatment using cell lines and patient-derived tumour cells.…”

Section: Bioprinted Tumour Modelmentioning

confidence: 99%

Modifying the Tumour Microenvironment: Challenges and Future Perspectives for Anticancer Plasma Treatments

Privat-Maldonado

Bengtson

Razzokov

et al. 2019

Cancers

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Tumours are complex systems formed by cellular (malignant, immune, and endothelial cells, fibroblasts) and acellular components (extracellular matrix (ECM) constituents and secreted factors). A close interplay between these factors, collectively called the tumour microenvironment, is required to respond appropriately to external cues and to determine the treatment outcome. Cold plasma (here referred as ‘plasma’) is an emerging anticancer technology that generates a unique cocktail of reactive oxygen and nitrogen species to eliminate cancerous cells via multiple mechanisms of action. While plasma is currently regarded as a local therapy, it can also modulate the mechanisms of cell-to-cell and cell-to-ECM communication, which could facilitate the propagation of its effect in tissue and distant sites. However, it is still largely unknown how the physical interactions occurring between cells and/or the ECM in the tumour microenvironment affect the plasma therapy outcome. In this review, we discuss the effect of plasma on cell-to-cell and cell-to-ECM communication in the context of the tumour microenvironment and suggest new avenues of research to advance our knowledge in the field. Furthermore, we revise the relevant state-of-the-art in three-dimensional in vitro models that could be used to analyse cell-to-cell and cell-to-ECM communication and further strengthen our understanding of the effect of plasma in solid tumours.

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Modeling Tumor Phenotypes In Vitro with Three-Dimensional Bioprinting

Cited by 186 publications

References 68 publications

Cell Culture Based in vitro Test Systems for Anticancer Drug Screening

Cell Culture Based in vitro Test Systems for Anticancer Drug Screening

Vascularized Biomaterials to Study Cancer Metastasis

Modifying the Tumour Microenvironment: Challenges and Future Perspectives for Anticancer Plasma Treatments

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