2003
DOI: 10.1242/jcs.00503
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Modeling tissue-specific signaling and organ function in three dimensions

Abstract: IntroductionQualitative evaluation of the cellular complexity and structural integrity of organ biopsies has been used by pathologists for decades to gain insight into human diseases. The well-accepted correlation between tissue structure and health or disease conveys important lessons for the development of experimental models for the study of normal human biology and associated disease progression. Optimally, model design should recapitulate both the 3D organization and the differentiated function of any giv… Show more

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Cited by 535 publications
(424 citation statements)
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“…15 In order to translate scientific results into clinical applications, 3D cell-based models and even 3D-organotypic models are needed. 16 Gene expression analysis is a common method to investigate molecular processes and regulatory events in cells, tissues, and organs. Within this analytical method, the application of quantitative reverse transcription polymerase chain reaction (qRT-PCR) is well established.…”
Section: Introductionmentioning
confidence: 99%
“…15 In order to translate scientific results into clinical applications, 3D cell-based models and even 3D-organotypic models are needed. 16 Gene expression analysis is a common method to investigate molecular processes and regulatory events in cells, tissues, and organs. Within this analytical method, the application of quantitative reverse transcription polymerase chain reaction (qRT-PCR) is well established.…”
Section: Introductionmentioning
confidence: 99%
“…There are several excellent reviews on the value of 3-D in vitro models (Schmeichel and Bissell, 2003;Debnath and Brugge, 2005;Yamada and Cukierman, 2007) …”
Section: Commentary Background Informationmentioning
confidence: 99%
“…Collectively, these studies show that the use of the RWV to generate 3-D cultures from a variety of cell types has wide applications in the modeling of infectious disease. (Schmeichel and Bissell, 2003). By analogy, 3-D cell culture holds enormous potential for novel product development for the diagnosis, prevention, and treatment of infectious disease.…”
Section: Use Of the Rwv Bioreactor To Engineer Biologically Meaningfumentioning
confidence: 99%
“…While these models continue to contribute to our understanding of infectious diseases, they are greatly limited in the extent to which they model the complexity of an intact 3-D tissue. In particular, this method of culture prevents the cells from establishing the 3-D architecture that is critical for the differentiated structure and function of parental tissues in vivo (Abbott, 2003;O'Brien et al, 2002;Schmeichel and Bissell, 2003;Zhang, 2004). In addition, cells grown as standard 2-D monolayers are also unable to respond to chemical and molecular gradients in three-dimensions (at apical, basal, and lateral cell surfaces) resulting in many departures from in vivo behavior (Zhang, 2004).…”
Section: Introductionmentioning
confidence: 99%