Modeling the reproductive impact of aquaculture-produced sexually fertile triploids on conspecific diploid populations

Winkler, Federico M.; Concha, Miguel L.; Concha, Christopher

doi:10.3354/aei00308

Cited by 1 publication

(1 citation statement)

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“…To minimize the potential genetic impact of hatchery-produced scallops on wild populations, there are different alternatives, including the systematic use of wild brooders (Yokota, Harada, & Iizuka, 2003), the use of completely genealogized brooders to ensure low inbreeding during a controlled reproduction process (Evans, Bartlett, Sweijd, Cook, & Elliott, 2004), the use of genetic markers to avoid inbred crosses (Liu et al, 2011), and the culture of triploids to prevent reproduction and genetic introgression in wild populations (Piferrer et al, 2009). However, this last method is not completely safe if the triploidization is not complete or if the triploids are not completely sterile (Winkler, Concha, & Concha, 2019).…”

Section: Discussionmentioning

confidence: 99%

Assessing the genetic diversity in Argopecten nucleus (Bivalvia: Pectinidae), a functional hermaphrodite species with extremely low population density and self‐fertilization: Effect of null alleles

Barros

Winkler

Velasco

2020

Ecology and Evolution

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Argopecten nucleus is a functional hermaphroditic pectinid species that exhibits self‐fertilization, whose natural populations have usually very low densities. In the present study, the genetic diversity of a wild population from Neguanje Bay, Santa Marta (Colombia), was estimated using microsatellite markers, and the effect of the presence of null alleles on this estimation was assessed. A total of 8 microsatellite markers were developed, the first described for this species, and their amplification conditions were standardized. They were used to determine the genotype of 48 wild individuals from Naguanje Bay, and 1,010 individuals derived from the offspring of 38 directed crosses. For each locus, the frequencies of the identified alleles, including null alleles, were estimated using the statistical package Micro‐Checker, and the parental genotypes were confirmed using segregation analysis. Three to 8 alleles per locus with frequencies from 0.001 to 0.632 were detected. The frequencies of null alleles ranged from 0.10 to 0.45, with Ho from 0.0 to 0.79, and He from 0.53 to 0.80. All loci were in H‐W disequilibrium. The null allele frequencies values were high, with lower estimations using segregation analysis than estimated using Micro‐Checker. The present results show high levels of population genetic diversity and indicate that null alleles were not the only cause of deviation from H‐W equilibrium in all loci, suggesting that the wild population under study presents signs of inbreeding and Wahlund effect.

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