Abstract:A number of studies have reported reversible ab-tubulin heterodimer dissociation with K d =10 À8 -10 À7 M, with presumably rapid but undetermined kinetics. However, in a recent study an extremely tight association between subunits (K d <10 À11 M), as well as a slow dissociation rate of the monomers was reported (with a half time for dissociation > 3 hours). In this work we have revisited the thermodynamics and kinetics of rat brain ab-tubulin (RBT) dissociation by taking advantage of sedimentation velocity ana… Show more
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