Modeling the exposure of wild fish to endocrine active chemicals: Potential linkages of total estrogenicity to field-observed intersex

Arlos, Maricor J.; Parker, Wayne; Bicudo, José R.; Law, Pam; Hicks, Keegan A.; Fuzzen, Meghan; Andrews, Susan A.; Servos, Mark R.

doi:10.1016/j.watres.2018.04.005

Cited by 34 publications

(12 citation statements)

References 63 publications

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“…However, non-lethal sampling of blood from fish for identification and quantification of miRNA conservatively requires a minimum fish mass of 500 g. This is due to the most conservative, non-lethal blood sampling guideline to take no more than 1 mL blood per 1 kg of total fish mass and the requirement of 0.5 mL of blood to collect the 0.2 mL plasma required to extract miRNA (Canadian Council on Animal Care, 2005;Hawkins et al, 2011;Lawrence et al, 2020). Additionally, many sentinel species are small-bodied fish, which would require lethal sampling to collect blood plasma, so less invasive methods are advised (Bahamonde et al, 2015;Thornton et al, 2017;Arlos et al, 2018). Mucus can be collected nonlethally from fish smaller than 500 g, though the amount of mucus that can be collected from a fish without causing unacceptable stress or resulting in infections is not yet defined (Canadian Council on Animal Care, 2005).…”

Section: Benefits and Limitations Of Each Non-lethal Sampling Locationmentioning

confidence: 99%

High Throughput Sequencing of MicroRNA in Rainbow Trout Plasma, Mucus, and Surrounding Water Following Acute Stress

et al. 2021

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Circulating plasma microRNAs (miRNAs) are well established as biomarkers of several diseases in humans and have recently been used as indicators of environmental exposures in fish. However, the role of plasma miRNAs in regulating acute stress responses in fish is largely unknown. Tissue and plasma miRNAs have recently been associated with excreted miRNAs; however, external miRNAs have never been measured in fish. The objective of this study was to identify the altered plasma miRNAs in response to acute stress in rainbow trout (Oncorhynchus mykiss), as well as altered miRNAs in fish epidermal mucus and the surrounding ambient water. Small RNA was extracted and sequenced from plasma, mucus, and water collected from rainbow trout pre- and 1 h-post a 3-min air stressor. Following small RNA-Seq and pathway analysis, we identified differentially expressed plasma miRNAs that targeted biosynthetic, degradation, and metabolic pathways. We successfully isolated miRNA from trout mucus and the surrounding water and detected differences in miRNA expression 1-h post air stress. The expressed miRNA profiles in mucus and water were different from the altered plasma miRNA profile, which indicated that the plasma miRNA response was not associated with or immediately reflected in external samples, which was further validated through qPCR. This research expands understanding of the role of plasma miRNA in the acute stress response of fish and is the first report of successful isolation and profiling of miRNA from fish mucus or samples of ambient water. Measurements of miRNA from plasma, mucus, or water can be further studied and have potential to be applied as non-lethal indicators of acute stress in fish.

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Section: Benefits and Limitations Of Each Non-lethal Sampling Locationmentioning

confidence: 99%

High Throughput Sequencing of MicroRNA in Rainbow Trout Plasma, Mucus, and Surrounding Water Following Acute Stress

et al. 2021

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“…Additionally, the estimated EEQ was higher than the NOECs for inducing intersex (1 ng/L) and VTG (5 ng/L) and even higher than the LOECs (the lowest observable effect concentrations) for inducing intersex (10 ng/L) and VTG (25 ng/L) selected by Jobling et al for their risk assessment [4]. Arlos et al estimated that an EEQ ≥10 ng-E2/L was associated with high intersex incidence and severity [54]. Therefore, concentrations of estrogens measured in this study in the target region are likely to have been high enough to cause intersex or VTG etc., which will affect the reproduction and ultimately the population size of fishes locally.…”

Section: Mass Fluxes Of Estrogens Along Rivers and From Wwtp Effluentsmentioning

confidence: 87%

Estrogens in municipal wastewater and receiving waters in the Beijing-Tianjin-Hebei region, China: Occurrence and risk assessment of mixtures

Lei

Lin

Zhu

et al. 2020

Journal of Hazardous Materials

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The potentially high release of estrogens to surface waters as a result of high population density and local livestock production in the Beijing-Tianjin-Hebei region may pose adverse effects on the reproductive systems of aquatic organisms. This study found that total measured concentrations of estrone (E1), 17β-estradiol (E2), estriol (E3), 17αethinylestradiol (EE2) and diethylstilbestrol (DES) were 468±27.2 ng/L in treated wastewater and 219±23 ng/L in river waters in this region. E2, E3 and EE2 were the predominant estrogens in river waters. The restriction of DES for human use should have been enforced, however concentrations of DES were relatively high compared to other studies. It has been estimated that the Haihe and Yongdingxin Rivers deliver approximately 1.8 tonnes of ∑estrogens to the Bohai Bay annually. Concentrations of individual estrogens were significantly higher in river waters in the dry season, however, mass loadings were significantly higher in the wet season. The E2-equivalent concentrations (EEQ) reached 1.2±0.16 and 0.64±0.08 µg-E2/L following long-term and short-term exposure estimates, respectively, in river waters with an average EE2 contribution of over 90%. This could potentially give rise to high risks to fish populations. The presence of estrogens in river waters largely derive from human excretion. Field studies on estrogenic effects on fish reproductive systems are required in this region as a result of high estrogen contamination levels.

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“…While the dual role of the sediments as a source and sink has been demonstrated for nutrients (e.g., Schindler & Krabbenhoft, 1998; Zarnetske et al., 2012), there has been little focus on how the source‐sink behavior modifies the spatiotemporal persistence of hormones that have been transported to agricultural streams in tile drainage and surface runoff. Indeed, most available stream models used to conduct risk assessments of hormonal compounds do not account for this dynamic source‐sink behavior of sediments (Anderson et al., 2012; Arlos et al., 2018; Green et al., 2013; Hannah et al., 2009; Klein, 2004; Williams et al., 2009; X. Zhao & Lung, 2017). One of the only exceptions to this is a modeling study of trenbolone by Ward et al.…”

Section: Introductionmentioning

confidence: 99%

Nevertheless, They Persisted: Can Hyporheic Zones Increase the Persistence of Estrogens in Streams?

Cheng

Preisendanz

Mashtare

et al. 2021

Water Resources Research

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Modeling the exposure of wild fish to endocrine active chemicals: Potential linkages of total estrogenicity to field-observed intersex

Cited by 34 publications

References 63 publications

High Throughput Sequencing of MicroRNA in Rainbow Trout Plasma, Mucus, and Surrounding Water Following Acute Stress

High Throughput Sequencing of MicroRNA in Rainbow Trout Plasma, Mucus, and Surrounding Water Following Acute Stress

Estrogens in municipal wastewater and receiving waters in the Beijing-Tianjin-Hebei region, China: Occurrence and risk assessment of mixtures

Nevertheless, They Persisted: Can Hyporheic Zones Increase the Persistence of Estrogens in Streams?

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