Modeling the early endometriotic lesion: mesothelium-endometrial cell co-culture increases endometrial invasion and alters mesothelial and endometrial gene transcription

Nair, Anitha S.; Nair, Hareesh B.; Lucidi, R.S.; Kirchner, A.J.; Schenken, Robert S.; Tekmal, Rajeshwar Rao; Witz, Craig A.

doi:10.1016/j.fertnstert.2007.09.047

Cited by 48 publications

(37 citation statements)

References 62 publications

(56 reference statements)

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“…Rather imaging technology has been effective for providing aid in surgical visualization of suspected lesions and adhesions [13,14]. One reason for the insufficiency of imaging technology to detect endometriotic lesions is due to the ability of the lesions to activate wound-healing mechanisms immediately following the attachment of the endometrial tissue fragments to the peritoneal surface of abdominal organs [15,16]. This "cloaking" mechanism nearly renders it impossible for imaging technology to discern normal tissue or scar tissue from an endometriotic lesion.…”

Section: How To Diagnose?mentioning

confidence: 99%

What Does Diagnosis of Endometriosis Mean? The Patient′s Perspective

Braundmeier¹,

Lenz²,

Fazleabas³

2016

JFIV Reprod Med Genet

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Endometriosis is a gynecological disease, which causes significant physiological pain but also psychological distress leading to decreased quality of life for those afflicted with the disease. Though researchers have investigated this disease for over 90 years, there is still much information unknown about its pathophysiology, hence impeding the development of effective treatment options. In addition, the difficulty to diagnose the disease using non-invasive measures increases the prevalence of this disease among reproductive aged women. This review discusses the challenges that the scientific and medical communities endure combating endometriosis along with the social and financial burdens of patients whom suffer from endometriosis. Finally this review highlights the positive and negative side effects of current treatment options, discusses some alternative treatment and new potential treatment options that have shown variable success in reducing the symptoms associated with endometriosis.

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Section: How To Diagnose?mentioning

confidence: 99%

What Does Diagnosis of Endometriosis Mean? The Patient′s Perspective

Braundmeier¹,

Lenz²,

Fazleabas³

2016

JFIV Reprod Med Genet

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“…The cells that did not invade through the Matrigel, were manually removed with cotton tip applicators. The invaded cells were visualized on the bottom of the coated membranes with a fluorescence microscope at Â20 objective (32). The relative fluorescence was quantified using Biorad-GS800 calibrated imaging densitometer installed with Quantity One software (Bio-Rad).…”

Section: Real-time Quantitative Rt-pcrmentioning

confidence: 99%

Gossypin as a Novel Selective Dual Inhibitor of v-raf Murine Sarcoma Viral Oncogene Homolog B1 and Cyclin-Dependent Kinase 4 for Melanoma

Bhaskaran

Dileep

Deepa

et al. 2013

Molecular Cancer Therapeutics

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Mutation in the BRAF gene (BRAFV600E) exists in nearly 70% of human melanomas. Targeted therapy against BRAFV600E kinase using a recently identified RAF-selective inhibitor, PLX4032, has been successful in early clinical trials. However, in patients with the normal BRAF allele (wild-type), PLX4032 is protumorigenic. This conundrum identifies the unmet need for novel therapeutic agents to target BRAFV600E kinase that are not counterproductive. We have identified gossypin, a pentahydroxy flavone, as a potent antimelanoma agent. Gossypin inhibited human melanoma cell proliferation, in vitro, in melanoma cell lines that harbor both BRAFV600E kinase and cyclin-dependent kinase 4 (CDK4) as well as in cells with BRAF wild-type allele. Gossypin inhibited kinase activities of BRAFV600E and CDK4, in vitro, possibly through direct binding of gossypin with these kinases, as confirmed by molecular docking studies. For cells harboring the BRAFV600E, gossypin inhibited cell proliferation through abrogation of the MEK-ERK-cyclin D1 pathway and in cells with BRAF wild-type allele, through attenuation of the retinoblastoma-cyclin D1 pathway. Furthermore, gossypin significantly inhibited melanoma growth in an organotypic three-dimensional skin culture mimicking human skin. Gossypin (10 and 100 mg/kg) treatment for 10 days in human melanoma (A375) cell xenograft tumors harboring BRAFV600E significantly reduced tumor volume through induction of apoptosis and increased survival rate in mice, and the effect was significantly superior to that of PLX4032 (10 mg/kg) or roscovitine 10 mg/kg. In summary, this study identified gossypin as a novel agent with dual inhibitory effects for BRAFV600E kinase and CDK4 for treatment of melanoma.

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“…The ESC invasion was evaluated as previously described elsewhere (12). The LP-9 PMCs were grown to confluence on growth factor-reduced Matrigel (BD Biosciences, San Jose, CA) coated membranes with 8-micron pores in 24-well plates.…”

Section: Invasion Assaymentioning

confidence: 99%

“…Labeled ESCs were plated at 50,000 cells per well in DMEM/F12 with and without imatinib (2 mM, 5 mM, and 10 mM) and were incubated at 37 C in 5% CO 2 for 24 hours. Noninvaded ESCs were mechanically removed from the upper surface of the membrane (12). Four nonoverlapping fields at Â10 microscopy were photographed.…”

Section: Invasion Assaymentioning

confidence: 99%

“…Once attached, ESCs and EECs penetrate the basement of peritoneal mesothelium and access the extracellular matrix within 24 hours (11). Invasion of ESCs through Matrigel-coated invasion chambers is significantly increased in the presence of a PMC monolayer, and this interaction alters expression of signaling kinase genes associated with invasion and metastasis (12). These findings suggest that cell-cell interactions affect cellular mechanisms that influence the invasive properties of endometrial cells.…”

mentioning

confidence: 95%

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