Modeling synchronization effects in the yeast cell cycle

Schlichting, Julia

doi:10.18452/19835

Cited by 1 publication

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“…We performed time-lapse imaging every 2 minutes and measured CLB2 mRNA expression throughout the cell cycle by acquiring z-stacks encompassing the cell volume ( Video 1 ). To reduce perturbations in gene expression due to synchronization protocols (Schlichting, 2019), we quantified CLB2 mRNA expression in unsynchronized cells, using the bud neck marker expression to compare cells. This revealed that up to 62.9% of CLB2 mRNAs localized in the bud ( Figures 2B and 2C ), consistent with the smFISH quantifications ( Figure 1E ).…”

Section: Resultsmentioning

confidence: 99%

Cyclin CLB2 mRNA localization and protein synthesis link cell cycle progression to bud growth

Tutucci

Maekiniemi

Snoep

et al. 2022

Preprint

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mRNA localization to subcellular compartments has been reported across all kingdoms of life and it is generally believed to promote asymmetric protein synthesis and localization. In striking contrast to previous observations, we show that in S. cerevisiae the B-type cyclin CLB2 mRNA is localized and translated in the yeast bud, while the Clb2 protein, a key regulator of mitosis progression, is concentrated in the mother nucleus. Using single-molecule RNA imaging in fixed (smFISH) and living cells (MS2 system), we show that the CLB2 mRNA is transported to the yeast bud by the She2-She3 complex, via an mRNA ZIP-code situated in the coding sequence. In CLB2 mRNA localization mutants, Clb2 protein synthesis in the bud is decreased resulting in changes in cell cycle distribution and genetic instability. Altogether, we propose that CLB2 mRNA localization acts as a sensor for bud development to couple cell growth and cell cycle progression, revealing a novel function for mRNA localization.

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Section: Resultsmentioning

confidence: 99%