Modeling Sugar Metabolism in Tomato Fruit

Mach, Jennifer

doi:10.1105/tpc.114.131177

Cited by 5 publications

(4 citation statements)

References 4 publications

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“…Even if five enzymes had a ratio close to 0.5 at the first stage of development (phosphofructokinase, V pfk ; glucose-6-phosphate dehydrogenase, V g6pdh ; invertase, V inv ; enolase, V eno ; and fructose 1,6–biphosphatase, V fbp ), no enzyme capacities were essential to run the model. The results obtained for enzymes in sugar cycling (invertase, sucrose synthase) were cross-validated with a kinetic model (Beauvoit et al ., 2014), and similar results have also been reported for B. napus embryos (Junker et al ., 2007) with enzyme activities present in a large surplus relative to their requirements for the observed in vivo fluxes. Given that most enzyme capacities varied quite dramatically throughout fruit development, we next investigated whether there were correlations between the measured V max and the corresponding calculated fluxes (Appendix S6).…”

Section: Resultssupporting

confidence: 88%

Modelling central metabolic fluxes by constraint‐based optimization reveals metabolic reprogramming of developing Solanum lycopersicum (tomato) fruit

et al. 2014

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Modelling of metabolic networks is a powerful tool to analyse the behaviour of developing plant organs, including fruits. Guided by our current understanding of heterotrophic metabolism of plant cells, a medium-scale stoichiometric model, including the balance of co–factors and energy, was constructed in order to describe metabolic shifts that occur through the nine sequential stages of Solanum lycopersicum (tomato) fruit development. The measured concentrations of the main biomass components and the accumulated metabolites in the pericarp, determined at each stage, were fitted in order to calculate, by derivation, the corresponding external fluxes. They were used as constraints to solve the model by minimizing the internal fluxes. The distribution of the calculated fluxes of central metabolism were then analysed and compared with known metabolic behaviours. For instance, the partition of the main metabolic pathways (glycolysis, pentose phosphate pathway, etc.) was relevant throughout fruit development. We also predicted a valid import of carbon and nitrogen by the fruit, as well as a consistent CO2 release. Interestingly, the energetic balance indicates that excess ATP is dissipated just before the onset of ripening, supporting the concept of the climacteric crisis. Finally, the apparent contradiction between calculated fluxes with low values compared with measured enzyme capacities suggest a complex reprogramming of the metabolic machinery during fruit development. With a powerful set of experimental data and an accurate definition of the metabolic system, this work provides important insight into the metabolic and physiological requirements of the developing tomato fruits.

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Section: Resultssupporting

confidence: 88%

Modelling central metabolic fluxes by constraint‐based optimization reveals metabolic reprogramming of developing Solanum lycopersicum (tomato) fruit

et al. 2014

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“…The diverse metabolites produced by plants can be divided into primary metabolites and secondary metabolites, in which primary metabolites play a central role in plant growth, cellular replenishment, resource allocation, and differentiation [8]. Primary metabolites include a wide range of intermediate compounds (such as sugars, organic acids, and amino acids) involved in glycolysis, the tricarboxylic acid (TCA) cycle, and amino acid metabolism [9]. Ascorbic acid (AsA) is an organic acid that scavenges reactive oxygen species and dietary AsA can reduce the incidence of important human diseases such as hypertension and diabetes [10].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide association analysis identifies a natural variation in basic helix-loop-helix transcription factor regulating ascorbate biosynthesis via D-mannose/L-galactose pathway in tomato

et al. 2019

PLoS Genet

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Tomato ( Solanum lycopersicum ) is one of the highest-value vegetable crops worldwide. Understanding the genetic regulation of primary metabolite levels can inform efforts aimed toward improving the nutrition of commercial tomato cultivars, while maintaining key traits such as yield and stress tolerance. We identified 388 suggestive association loci (including 126 significant loci) for 92 metabolic traits including nutrition and flavor-related loci by genome-wide association study from 302 accessions in two different environments. Among them, an ascorbate quantitative trait locus TFA9 ( T OMATO F RUIT A SCORBATEON CHROMOSOME 9 ) co-localized with SlbHLH59 , which promotes high ascorbate accumulation by directly binding to the promoter of structural genes involved in the D-mannose/L-galactose pathway. The causal mutation of TFA9 is an 8-bp InDel, named InDel_8, located in the promoter region of SlbHLH59 and spanned a 5’UTR Py-rich stretch motif affecting its expression. Phylogenetic analysis revealed that differentially expressed SlbHLH59 alleles were selected during tomato domestication. Our results provide a dramatic illustration of how ascorbate biosynthesis can be regulated and was selected during the domestication of tomato. Furthermore, the findings provide novel genetic insights into natural variation of metabolites in tomato fruit, and will promote efficient utilization of metabolite traits in tomato improvement.

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“…Invertase and sucrose synthase, along with the other regulated enzymes of the starch and sucrose metabolism in the present study seem to play a major role during early development of tomato fruits. It is well-known that sucrose, fructose and glucose are stored in the cell vacuole (Mach, 2014). activity of the enzyme also results in the synthesis of D-glucose and D-galactose in the "galactose metabolism" pathway (data provided upon request).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Dynamics of Early Developing Tomato (Solanum Lycopersicum L.) Fruit Based on Rna-Seq Analysis

Edris¹

2019

Appl. Ecol. Env. Res.

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Tomato (Solanum lycopersicum L.) is one of the most popular vegetable species grown worldwide. Functional genomic analysis of genes in the early flower and fruit stages are the key factors for genetic enhancement of tomato fruits for increased yield and quality. In the current study, RNA-Seq datasets were generated for the Chico cultivar at six growth stages, 0, 3, 5, 7, 9 and 12 days after pollination (DAP). The computational analysis of transcriptomes and heat map showed close relationships among transcripts regulated at 3 and 5 DAP and at 7 and 9 DAP, whereas transcripts regulated at 12 DAP were more distant. Annotated transcripts and KEGG analysis showed that starch and sucrose pathway experienced the most dynamic changes resulting in the synthesis of several soluble sugars, e.g., sucrose, -D-fructose, -D-glucose, trehalose, maltose and 1,4--D-xylan. Enzymes

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Modeling Sugar Metabolism in Tomato Fruit

Cited by 5 publications

References 4 publications

Modelling central metabolic fluxes by constraint‐based optimization reveals metabolic reprogramming of developing Solanum lycopersicum (tomato) fruit

Modelling central metabolic fluxes by constraint‐based optimization reveals metabolic reprogramming of developing Solanum lycopersicum (tomato) fruit

Genome-wide association analysis identifies a natural variation in basic helix-loop-helix transcription factor regulating ascorbate biosynthesis via D-mannose/L-galactose pathway in tomato

Transcriptional Dynamics of Early Developing Tomato (Solanum Lycopersicum L.) Fruit Based on Rna-Seq Analysis

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