Modeling, Simulation, and Performance Evaluation of a Novel Microfluidic Impedance Cytometer for Morphology-Based Cell Discrimination

Caselli, Federica; Shaker, Marjan; Colella, Ludovica; Renaud, Philippe; Bisegna, Paolo

doi:10.1109/jmems.2014.2325979

Cited by 20 publications

(15 citation statements)

References 35 publications

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“…This ratio can be used to provide an independent measurement of particle height which can be used to correct for the impedance signal. The system was characterised using finite element simulations to determine the field and current -details of the computational model of a typical impedance cytometer can be found elsewhere 1,[30][31][32] (also see ESI † ). The simulations were performed using the dimensions of the actual experimental microfluidic chip, which had a channel with approximate dimensions of 36 µm (wide) × 45 µm (high), with 30 µm wide electrodes and 10 µm spacing, and using the electrical parameters shown in Table S1 † .…”

Section: Operating Principlementioning

confidence: 99%

High accuracy particle analysis using sheathless microfluidic impedance cytometry

et al. 2016

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This paper describes a new design of microfluidic impedance cytometer enabling accurate characterization of particles without the need for focusing. The approach uses multiple pairs of electrodes to measure the transit time of particles through the device using two simultaneous different current measurements, a transverse (top to bottom) current and an oblique current. This gives a new metric that can be used to estimate the vertical position of the particle trajectory through the microchannel. This parameter effectively compensates for the non-uniform electric field in the channel that is an unavoidable consequence of the use of planar parallel facing electrodes. The new technique is explained and validated using numerical modelling. Impedance data for 5, 6 and 7 µm particles are collected and compared with simulations. The method gives excellent Coefficient of Variation in (electrical) radius of particles of 1% for a sheath less configuration.

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Section: Operating Principlementioning

confidence: 99%

High accuracy particle analysis using sheathless microfluidic impedance cytometry

et al. 2016

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“…A triangular mesh of the cell membrane Γ is generated and used as boundary mesh in the creation of tetrahedral meshes of the intracellular and fluid domains (respectively, Ω 1 and Ω 2 ). Treating the cell membrane as a two-dimensional interface avoids the need of extra-fine tetrahedral meshes as required in a three-dimensional description of a thin region [2,9,10]. Weak Form PDE Physics is adopted, and quadratic Lagrangian elements are used to interpolate the electric potential Ψ .…”

Section: Electrical Modulementioning

confidence: 99%

“…In the electrical module, field-induced actions are rigorously computed by means of the Maxwell stress tensor theory (Section 2.1). Moreover, in order to evaluate the electric field distribution (Section 2.2), the cell membrane is treated as a two-dimensional imperfect interface, so that the computational cost is significantly reduced [2,9,10]. As a byproduct, an accurate computation of the Maxwell stress tensor is achieved (Section 4.1).…”

Section: Introductionmentioning

confidence: 99%

Effective computational modeling of erythrocyte electro-deformation

2016

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Due to its crucial role in pathophysiology, erythrocyte deformability represents a subject of intense experimental and modeling research. Here a computational approach to electro-deformation for erythrocyte mechanical characterization is presented. Strong points of the proposed strategy are: i) an accurate computation of the mechanical actions induced on the cell by the electric field, ii) a microstructurally-based continuum model of the erythrocyte mechanical behavior, iii) an original rotation-free shell finite element, especially suited to the application in hand. As proved by the numerical results, the developed tool is effective and sound, and can foster the role of electro-deformation in single-cell mechanical phenotyping.

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“…23 This innovative system represents a significant step forward in the state-of-the-art microcytometers' development. Morphology sensing in parallel with viability monitoring and counting significantly enhances the discrimination capabilities of modern cytometers.…”

Section: Lab Chipmentioning

confidence: 99%

An impedance-based flow microcytometer for single cell morphology discrimination

et al. 2014

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Cell shape is a fundamental biological feature, providing specific information about physiological or pathological cellular conditions. Most of the state-of-the-art microfluidic cytometers, however, only allow simple cell analysis, including viability studies, cell counting and sorting. In this work, we present a non-invasive, label-free device capable of single cell morphology discrimination in continuous flow. The device is based on the principle of liquid electrodes, fabricated in a cross configuration around a sensing zone. This arrangement allows measurement of cell impedance along orthogonal orientations and extraction of an index describing cell shape anisotropy. By adding prior to the sensing volume a series of lateral liquid electrodes, the particle stream was focused toward the channel midline and each cell was oriented in a specific direction before shape sensing. We demonstrate the proof of concept by performing spherical and elongated particle discrimination. As an application, we show that the shape changes experienced during cell division can be monitored and characterized. In particular, budding yeasts at different stages of the mitotic cycle were identified by extracting their anisotropy index.

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Modeling, Simulation, and Performance Evaluation of a Novel Microfluidic Impedance Cytometer for Morphology-Based Cell Discrimination

Cited by 20 publications

References 35 publications

High accuracy particle analysis using sheathless microfluidic impedance cytometry

High accuracy particle analysis using sheathless microfluidic impedance cytometry

Effective computational modeling of erythrocyte electro-deformation

An impedance-based flow microcytometer for single cell morphology discrimination

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