Modeling sequential ammonia oxidation kinetics in enriched nitrifying microbial culture

Abstract

BACKGROUND: This work evaluated the kinetics of stepwise ammonia oxidation, separately accounting for ammonia oxidizers (AOB) and nitrite oxidizers (NOB) in an enriched nitrifying microbial culture. This culture was sustained in a fill and draw reactor fed with 50 mg N L −1 of ammonia together with limited organic substrate. Batch experiments were started with the enriched biomass and the same ammonia feeding to monitor profiles of oxygen uptake rate and resulting nitrogen fractions.

Chronic impact of tetracycline on nitrification kinetics and the activity of enriched nitrifying microbial culture

“…In the second phase, a second fill and draw reactor was started with acclimated biomass from the previous system, this time to obtain an enriched nitrifier community, where metabolic activities of ammonia oxidizers and nitrite oxidizers could be separately observed and their behaviour interpreted. After steady-state conditions were reached, as seen by Katipoglu-Yazan et al (2015), the reactor operation was continued with a daily TET dosing of 50 mg/L for 45 days e the acclimation phase e with periodic monitoring of microbial w a t e r r e s e a r c h x x x ( 2 0 1 5 ) 1 e1 2 diversity change, abundance of nitrifying bacteria as well as amoA and 16S rRNA gene activity, using molecular techniques.…”

“…The feed solution constituents were listed elsewhere (Katipoglu-Yazan et al, 2015). In addition to peptone mixture, macro and micro nutrients were also supplied in order to support microbial growth and maintain pH at neutral levels.…”

“…Conventionally, inhibition has been evaluated on the basis of observed changes in the process kinetics and accordingly, extensive research has been reported on nitrification kinetics (Sin et al, 2008). Lately, respirometric analysis has provided a new dimension for a more accurate interpretation of process kinetics, both for heterotrophic and autotrophic microbial communities (Katipoglu-Yazan et al, 2015). However, related evaluations of respirometric analyses have not been supported and complemented by molecular studies that would reveal parallel changes in the composition and activity of the microbial community and the true nature of the inhibitory impact on bacterial components of the microbial ecology.…”

Dynamic simulation for wastewater treatment plants management: Case of Souk-Ahras region, north-eastern Algeria

“…The adjusted parameters are introduced manually, taking into consideration their different interactions according to the following two principles: (i) a parameter is adjusted during the phase where the relative process is clearly dominant and (ii) biomass efficiencies are assumed to be correct in adjusting the decay kinetics. These parameters are usually estimated by means of respirometric tests [ELSHORBAGY, SHAWA-QFAH 2015; KATIPOGLU-YAZAN et al 2015]. Indeed, the rate of breath is directly related to two important biochemical processes which must be controlled in a WWTP: growth of biomass and substrate consumption [SPANJERS et al 1996].…”

Implementing a respirometry-based model into BioWin software to simulate wastewater treatment plant operations

“…These parameters are usually evaluated by means of respirometric tests [4,6,[15][16][17][18]. Indeed, respiration rate is directly linked to two important biochemical processes that must be controlled in a WWTP: biomass growth and substrate consumption [19].…”