Abstract:Ineffective heterologous protein synthesis has often been ascribed to codon bias and rare codons. New experimental evidence suggests that codon bias alone may not be the sole cause of poor translation. In this paper we present a free-energy based model of translation elongation to predict and optimize genes for expression in E. coli. The model takes into account second order free energy effects from the binding between the anti-Shine-Dalgarno sequence of the 3' terminal 16S rRNA tail and the mRNA, tRNA abundan… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.