Modeling psychiatric comorbid symptoms of epileptic seizures in zebrafish

Canzian, Julia; Müller, Talise E.; Franscescon, Francini; Michelotti, Paula; Fontana, Bárbara D.; Costa, Fabiano V.

doi:10.1016/j.jpsychires.2019.09.007

Cited by 22 publications

(7 citation statements)

References 67 publications

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“…The learning deficits described in the current study after larval seizures are consistent with studies reporting changes in learning and memory after seizures induced in adult zebrafish (Lee et al, 2010;Canzian et al, 2019). Zebrafish capacity for learning and memory is broad and well-studied.…”

Section: Discussionsupporting

confidence: 90%

“…Specific to cognitive and behavioral investigations, zebrafish are social animals with a demonstrated capacity for learning (Cognato Gde et al, 2012), and are useful for modeling neurodevelopmental disorders (de Abreu et al, 2020). In adult zebrafish, chemicallyinduced seizures can result in deficits in learning six to 24 h after seizure induction, and increased anxiety and aggression 30 days after seizures (Lee et al, 2010;Canzian et al, 2019;Budaszewski Pinto et al, 2021). However, it is unclear how much of the data collected using the larval seizure model can be applied to the cognitive and behavioral consequences of ELS since long-term behavior and cognition following larval seizures have not yet been characterized.…”

Section: Introductionmentioning

confidence: 99%

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Learning Deficits and Attenuated Adaptive Stress Response After Early-Life Seizures in Zebrafish

Singh

Ramon²,

Finore³

et al. 2022

Front. Neurosci.

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Early-life seizures (ELS) are often associated with the development of cognitive deficits. However, methods to predict and prevent these deficits are lacking. To increase the range of research models available to study cognitive consequences of ELS, we investigated whether seizures in larval zebrafish (Danio rerio) lead to behavioral deficits later in life. We thus modified the existing pentylenetetrazole (PTZ)-induced seizure model in larval zebrafish, exposing zebrafish to PTZ daily from 5 to 7 days post-fertilization (dpf). We then compared later-life learning, social behavior (shoaling), and behavioral and chemical measures of anxiety in the PTZ-exposed zebrafish (PTZ group) to that of naïve clutchmates (untouched controls, UC) and to a second control group (handling control, HC) that experienced the same handling as the PTZ group, but without PTZ exposure. We observed that only the PTZ group displayed a significant deficit in a y-maze learning task, while only the HC group displayed a social deficit of decreased shoaling. HC fish also showed an increased frequency of behavioral freezing and elevated cortisol responses to netting, heightened stress responses not seen in the PTZ fish. Since mild stressors, such as the handling the HC fish experienced, can lead to learned, advantageous responses to stress later in life, we tested escape response in the HC fish using an acoustic startle stimulus. The HC group showed an enhanced startle response, swimming significantly farther than either the PTZ or UC group immediately after being startled. Taken together, these results indicate that seizures in larval zebrafish impair learning and the development of an adaptive, heightened stress response after early-life stress. These findings expand the behavioral characterization of the larval zebrafish seizure model, strengthening the power of this model for ELS research.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Learning Deficits and Attenuated Adaptive Stress Response After Early-Life Seizures in Zebrafish

Singh

Ramon²,

Finore³

et al. 2022

Front. Neurosci.

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“…A high stress level has been reported in 23.3% of our patients. The anxiety-like symptoms were high in most people with epilepsy in the study by Canzian et al 21 …”

Section: Discussionmentioning

confidence: 86%

“…A high stress level has been reported in 23.3% of our patients. The anxiety-like symptoms were high in most people with epilepsy in the study by Canzian et al 21 Our study was limited by a small sample size and included only one city. More studies are needed to include more cities in Saudi Arabia to attain more accurate representative results.…”

Section: Methodsmentioning

confidence: 87%

Prevalence of depression among epileptic patients in Taif City, Saudi Arabia

Mubaraki

Sibyani

Alkhawtani

et al. 2021

NSJ

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Objectives: To assess depression and its relation with different factors among people with epilepsy in wider range of ages. Methods:A cross-sectional study was conducted in Taif city. The sample size was 150; the inclusion criteria were ≥18-year-old patients diagnosed with epilepsy. The data were collected by interviewing patients following up from rural and urban areas of Taif in neurology clinics at King Faisal Medical Complex )KFMC( and King Abdul-Aziz Specialist Hospital )KAASH(. The PHQ-9 score was used to screen for depression.

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“…Although it is generally believed that about two-thirds of epilepsy is idiopathic, most of which are now considered to be hereditary[ 1 ]. Epilepsy also has various psychiatric complications such as depression, anxiety and cognitive defects[ 3 , 4 ]. It has been reported that peroxisome proliferator-activated receptor γ and mutations in genes involved in GABA-mediated inhibitory neurotransmission are highly associated with the pathogenesis of epilepsy[ 1 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of three administration modes for establishing a zebrafish seizure model induced by N-Methyl-D-aspartic acid

Long

Wang

Luo

et al. 2020

WJP

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BACKGROUND Epilepsy is a complex neurological disorder characterized by recurrent, unprovoked seizures resulting from the sudden abnormal discharge of brain neurons. It leads to transient brain dysfunction, manifested by abnormal physical movements and consciousness. It can occur at any age, affecting approximately 65 million worldwide, one third of which are still estimated to suffer from refractory seizures. There is an urgent need for further establishment of seizure models in animals, which provides an approach to model epilepsy and could be used to identify novel anti-epileptic therapeutics in the future. AIM To compare three administration modes for establishing a seizure model caused by N-Methyl-D-aspartic acid (NMDA) in zebrafish. METHODS Three administration routes of NMDA, including immersion, intravitreal injection and intraperitoneal injection, were compared with regard to their effects on inducing seizure-like behaviors in adult zebrafish. We evaluated neurotoxicity by observing behavioral changes in zebrafish and graded those behaviors with a seizure score. In addition, the protective effects of MK-801 (Dizocilpine) and natural active constituent resveratrol against NMDA-induced alterations were studied. RESULTS The three NMDA-administration methods triggered different patterns of the epileptic process in adult zebrafish. Seizure scores were increased after increasing NMDA concentration regardless of the mode of administration. However, the curve of immersion continuously rose to a high plateau (after 50 min), while the curves of intravitreal injection and intraperitoneal injection showed a spike in the early stage (10-20 min) followed by a steady decrease in seizure scores. Furthermore, pretreatment with resveratrol and MK-801 significantly delayed seizure onset time and lowered seizure scores. CONCLUSION By comparing the three methods of administration, intravitreal injection of NMDA was the most suitable for establishing an acute epileptic model in zebrafish. Thus, intraperitoneal injection in zebrafish can be applied to simulate diseases such as epilepsy. In addition, NMDA immersion may be an appropriate method to induce persistent seizures. Moreover, MK-801 and resveratrol showed strong anti-epileptic effects; thus, both of them may be clinically valuable treatments for epilepsy.

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Modeling psychiatric comorbid symptoms of epileptic seizures in zebrafish

Cited by 22 publications

References 67 publications

Learning Deficits and Attenuated Adaptive Stress Response After Early-Life Seizures in Zebrafish

Learning Deficits and Attenuated Adaptive Stress Response After Early-Life Seizures in Zebrafish

Prevalence of depression among epileptic patients in Taif City, Saudi Arabia

Comparison of three administration modes for establishing a zebrafish seizure model induced by N-Methyl-D-aspartic acid

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