Modeling Podocyte Ontogeny and Podocytopathies with the Zebrafish

Drummond, B. J.; Ercanbrack, Wesley S.; Wingert, Rebecca A.

doi:10.3390/jdb11010009

Cited by 5 publications

(2 citation statements)

References 276 publications

(499 reference statements)

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“…Within the context of the kidney, CB1 activates the MAPK signaling and promotes cisplatin-induced nephropathy, which will be discussed later in this review [50]. Interestingly, the activation of the PI3K/AKT pathway is associated with injury to podocytes, which are crucial for kidney function and health [51], although a formal link between CB1 and podocyte injury via PI3K/Akt activation has yet to be made [22,52,53]. The ECS has been associated with other cellular pathways that are involved in oxidative stress; however, these will not be discussed for the purposes of this review.…”

Section: Cellular Processes Of the Ecsmentioning

confidence: 99%

Cannabinoid Signaling in Kidney Disease

Arceri

Nguyen

Gibson

et al. 2023

Cells

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Endocannabinoid signaling plays crucial roles in human physiology in the function of multiple systems. The two cannabinoid receptors, CB1 and CB2, are cell membrane proteins that interact with both exogenous and endogenous bioactive lipid ligands, or endocannabinoids. Recent evidence has established that endocannabinoid signaling operates within the human kidney, as well as suggests the important role it plays in multiple renal pathologies. CB1, specifically, has been identified as the more prominent ECS receptor within the kidney, allowing us to place emphasis on this receptor. The activity of CB1 has been repeatedly shown to contribute to both diabetic and non-diabetic chronic kidney disease (CKD). Interestingly, recent reports of acute kidney injury (AKI) have been attributed to synthetic cannabinoid use. Therefore, the exploration of the ECS, its receptors, and its ligands can help provide better insight into new methods of treatment for a range of renal diseases. This review explores the endocannabinoid system, with a focus on its impacts within the healthy and diseased kidney.

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Section: Cellular Processes Of the Ecsmentioning

confidence: 99%

Cannabinoid Signaling in Kidney Disease

Arceri

Nguyen

Gibson

et al. 2023

Cells

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“…Each of these kidney structures originates from populations of mesodermally derived renal progenitors. The zebrafish pronephros is extremely simple, being comprised of two nephrons that form rapidly and function throughout the first several weeks of life [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 ]. Key transcription factors such as Pax2a, Pax8, Hand1 and Osr1 have critical roles in the specification of the renal progenitor’s fate [ 55 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 ].…”

Section: Using the Zebrafish To Study Nephron Developmentmentioning

confidence: 99%

Principles of Zebrafish Nephron Segment Development

Nguyen

Petrikas

Chambers

et al. 2023

JDB

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Nephrons are the functional units which comprise the kidney. Each nephron contains a number of physiologically unique populations of specialized epithelial cells that are organized into discrete domains known as segments. The principles of nephron segment development have been the subject of many studies in recent years. Understanding the mechanisms of nephrogenesis has enormous potential to expand our knowledge about the basis of congenital anomalies of the kidney and urinary tract (CAKUT), and to contribute to ongoing regenerative medicine efforts aimed at identifying renal repair mechanisms and generating replacement kidney tissue. The study of the zebrafish embryonic kidney, or pronephros, provides many opportunities to identify the genes and signaling pathways that control nephron segment development. Here, we describe recent advances of nephron segment patterning and differentiation in the zebrafish, with a focus on distal segment formation.

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Kif21a deficiency leads to impaired glomerular filtration barrier function

Riedmann,

Kayser,

Helmstädter

et al. 2023

Sci Rep

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The renal glomerulus represents the major filtration body of the vertebrate nephron and is responsible for urine production and a number of other functions such as metabolic waste elimination and the regulation of water, electrolyte and acid–base balance. Podocytes are highly specialized epithelial cells that form a crucial part of the glomerular filtration barrier (GFB) by establishing a slit diaphragm for semipermeable plasma ultrafiltration. Defects of the GFB lead to proteinuria and impaired kidney function often resulting in end-stage renal failure. Although significant knowledge has been acquired in recent years, many aspects in podocyte biology are still incompletely understood. By using zebrafish as a vertebrate in vivo model, we report a novel role of the Kinesin-like motor protein Kif21a in glomerular filtration. Our studies demonstrate specific Kif21a localization to the podocytes. Its deficiency resulted in altered podocyte morphology leading to podocyte foot process effacement and altered slit diaphragm formation. Finally, we proved considerable functional consequences of Kif21a deficiency by demonstrating a leaky GFB resulting in severe proteinuria. Conclusively, our data identified a novel role of Kif21a for proper GFB function and adds another piece to the understanding of podocyte architecture and regulation.

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Modeling Podocyte Ontogeny and Podocytopathies with the Zebrafish

Cited by 5 publications

References 276 publications

Cannabinoid Signaling in Kidney Disease

Cannabinoid Signaling in Kidney Disease

Principles of Zebrafish Nephron Segment Development

Kif21a deficiency leads to impaired glomerular filtration barrier function

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