Modeling of the jasmonate signaling pathway in Arabidopsis thaliana with respect to pathophysiology of Alternaria blight in Brassica

Abstract

The productivity of Oilseed Brassica, one of the economically important crops of India, is seriously affected by the disease, Alternaria blight. The disease is mainly caused by two major necrotrophic fungi, Alternaria brassicae and Alternaria brassicicola which are responsible for significant yield losses. Till date, no resistant source is available against Alternaria blight, hence plant breeding methods can not be used to develop disease resistant varieties. Jasmonate mediated signalling pathway, which is known to play crucial role during defense response against necrotrophs, could be strengthened in Brassica plants to combat the disease. Since scanty information is available in Brassica-Alternaria pathosystems at molecular level therefore, in the present study efforts have been made to model jasmonic acid pathway in Arabidopsis thaliana to simulate the dynamic behaviour of molecular species in the model. Besides, the developed model was also analyzed topologically for investigation of the hubs node. COI1 is identified as one of the promising candidate genes in response to Alternaria and other linked components of plant defense mechanisms against the pathogens. The findings from present study are therefore informative for understanding the molecular basis of pathophysiology and rational management of Alternaria blight for securing food and nutritional security.

Prior exposure of Arabidopsis seedlings to mechanical stress heightens jasmonic acid-mediated defense against necrotrophic pathogens

“…JA mediates MS-induced resistance against A. brassicicola MS enhances JA accumulation ( Fig. 1D), which can mediate resistance against insect attack and necrotrophic pathogen infection [16,34,38]. Therefore, we examined whether MS-induced resistance against A. brassicicola required JA signalling.…”

Understanding Host–Pathogen Interactions in Brassica napus in the Omics Era

“…Metabolites associated with black rot infection in B. oleracea were identified using liquid chromatography-quadrupole time-of-flight (LC-QTOF)-based metabolite profiling, which revealed that metabolic changes in the host occurred 48 h after infection and implicated photosynthesis, alkaloids, coumarins and sphingolipids as involved during the infection process [ 215 ]. Systems biology was constructed to model the metabolic pathway for JA signalling in the Brassica – Alternaria pathophysiology to identify important elements in the regulation of resistance mechanisms and to pinpoint molecular targets for engineering enhanced resistance in Brassica crops [ 216 ].…”

Systems biology of seeds: decoding the secret of biochemical seed factories for nutritional security

“…Advances in high-throughput 'omics' technologies have generated a huge amount of biological data related to seed, and significant efforts have been made to understand biological systems as true systems for a cost-effective and affordable systems biology study. Analysis of high-throughput omics data through bioinformatics approaches involving database handling, modeling, network analysis and simulation results tremendous progress in system-level understanding of biological systems (Kumar et al 2015b;Gupta and Misra 2016;Pathak et al 2017). Systems biology is essential to understand the molecular basis of seed from molecular to cellular and systems level for dissecting the intricacy of each component and its specific role in developmental processes and assist to increasing of the nutrient production in seeds through bio-fortification, metabolic engineering and other biotechnological approaches that will definitely help in nutritional security (Fig.…”