Modeling of the interaction between osteoblasts and biocompatible substrates as a function of adhesion strength

Abstract

A goal of current implantology research is to design devices that induce controlled, guided, and rapid healing. Nanoscale structured substrates [e.g., titania nanotubes (TNTs) or carbon nanotubes (CNTs)] dramatically improve the functions of conventional biomaterials. The present investigation evaluated the behavior of osteoblasts cells cultured on smooth and nanostructured substrates, by measuring osteoblasts specific biomarkers [alkaline phosphatase (AP) and total protein] and cells adhesion strength to substrates, followed by semi-empirical modeling to predict the experimental results. Findings were in total agreement with the current state of the art. The proliferation, as well as the AP and total protein levels were higher on the nanostructure phases (TNTs, CNTs) comparing to the smooth ones (plastic and pure titanium). Cells adhesion strength measured was found higher on the nanostructured materials. This coincided with a higher value of proteins which are directly implicated in the process of adherence. Results were accurately predicted through the Viscoelastic Hybrid Interphase Model. A gradual adherence of bone cells to implants using multilayered biomaterials that involve biodegradable polymeric films and a nanoscale modification of titanium surface is suggested to improve performance through an interphase-mediated osteointegration of orthopedic implants. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 621-628, 2018.

Mechanical Behavior of Bi-Layer and Dispersion Coatings Composed of Several Nanostructures on Ti13Nb13Zr Alloy

“…Sasani et al [ 28 ] used copper decorated multi-wall carbon nanotubes (MWCNTs) to incorporate fluorapatite-titania coating onto a Ti-based implant, which improved composite coating in terms of nanomechanical properties. The toxicity profile and biocompatibility of CNTs were the main concerns as confirmed by numerous reports available on this issue [ 2 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. The size (diameter and length [ 30 , 31 ]) and surface area of the CNTs play an important role in toxicology—the smaller the size structure and increased surface area, the larger probability of the interactions with living cells and translocation into the cells, thus adverse biological effects.…”

Combined Optimized Effect of a Highly Self-Organized Nanosubstrate and an Electric Field on Osteoblast Bone Cells Activity

“…On the other hand, electrically stimulated osteoblasts present decreased TP level when on metallic substrates, thus adjusting adhesion to confer mobility. In the case of TNTs (Figure 4(b)), a phenomenon takes place that has already been described and modelled [15] through the concept of the adhesion efficiency. On metallic materials, a low TP level is preferred, because this will increase the “ALP/TP” ratio.…”

“…The ALP activity and TP levels have been measured after one and three days of incubation in nonstimulated and electrically stimulated osteoblasts. As previously described in [14, 15], ALP is related to osteoblasts early differentiation, while TP is related to the proliferation and the degree of adhesion of the cells on the substrate. Both parameters are crucial in the development of new biomaterials aimed to induce rapid and efficient osteointegration.…”

“…Human bone cells (osteoblasts) were isolated from residual bone obtained from a 47 years old female patient, during knee surgery. The cell suspension was cultured until confluence in medium described in [14, 15]. During multiplication, proliferation, and while seeded on TCP substrate, osteoblasts cultures were observed with an inverted optical microscope (Nikon Diaphot).…”

Electrospun TiO2 nanofiber integrated lab-on-a-disc for ultrasensitive protein detection from whole blood

“…Recently, Titanium dioxide (TiO 2 )-based nanomaterials have 60 been proposed as excellent candidates for biosensing, 8,14,15 as they display remarkable properties such as chemical stability, 16 negligible protein denaturation, 17 biocompatibility, 18 and more importantly the versatility in functionalization. 19,20 The TiO 2 NFs can be easily fabricated by electrospinning.…”