Modeling of Process Parameters for Enhanced Production of Coenzyme Q10 FromRhodotorula glutinis

Abstract

Coenzyme Q10 (CoQ10) plays an indispensable role in ATP generation through oxidative phosphorylation and helps in scavenging superoxides generated during electron transfer reactions. It finds extensive applications specifically related to oxidative damage and metabolic dysfunctions. This article reports the use of a statistical approach to optimize the concentration of key variables for the enhanced production of CoQ10 by Rhodotorula glutinis in a lab-scale fermenter. The culture conditions that promote optimum growth and CoQ10 production were optimized and the interaction of significant variables para-hydroxybenzoic acid (PHB, 819.34 mg/L) and soybean oil (7.78% [v/v]) was studied using response surface methodology (RSM). CoQ10 production increased considerably from 10 mg/L (in control) to 39.2 mg/L in batch mode with RSM-optimized precursor concentration. In the fed-batch mode, PHB and soybean oil feeding strategy enhanced CoQ10 production to 78.2 mg/L.

Environmental and Genetic Factors Associated with Solanesol Accumulation in Potato Leaves

“…In the pharmaceutical industry solanesol is used as an intermediate in the synthesis (both chemically and biotechnologically) of metabolically active quinones such as coenzyme Q10 and vitamin K analogs (Naruta, 1980; Hamamura et al, 2002; Choi et al, 2005; Tian et al, 2010; Athiyaman and Sankaranarayanan, 2014). There is also a growing awareness that solanesol may have useful properties in its own right with reports of anti-bacterial, anti-inflammation, and anti-ulcer activities (Serebryakov and Nigmatov, 1990; Khidyrova and Shakhidoyatov, 2002; Guo et al, 2008; Li and Chase, 2010).…”

CoQ10 a super-vitamin: review on application and biosynthesis

“…Balakumaran and Meenakshisundaram (2015) significantly enhanced CoQ10 production from 10 mgL (in control) to 39.2 mg/L in batch mode with RSM-optimized precursor concentration. Using fed-batch mode, PHB and soybean oilfeeding strategy they obtained enhanced CoQ10 production (78.2 mg/L) (Balakumaran and Meenakshisundaram 2015). Lee et al (2017) have recently discussed various strategies currently adopted to overproduce CoQ10 and have discussed some of the potential novel areas for future research.…”

“…Improved CoQ10 production arising by lactate supplementation was ascribed to the high cell concentration and specific CoQ10 content derived from the acceleration of TCA cycle and energy production (Ha et al 2008). Balakumaran and Meenakshisundaram (2015) significantly enhanced CoQ10 production from 10 mgL (in control) to 39.2 mg/L in batch mode with RSM-optimized precursor concentration. Using fed-batch mode, PHB and soybean oilfeeding strategy they obtained enhanced CoQ10 production (78.2 mg/L) (Balakumaran and Meenakshisundaram 2015).…”

Tobacco as bioenergy and medical plant for biofuels and bioproduction

“…Additionally, solanesol derived from tobacco has been shown to promote cell growth and enhance coenzyme Q 10 production [ 48 ]. Coenzyme Q 10 is an antioxidant dietary supplement with the effects of anti-inflammatory [ 49 ], anti-tumor [ 50 , 51 ], antihypertension, treating cardiovascular disease [ 52 ] and alleviating abnormal metabolic functions [ 53 ]. Coenzyme Q 10 has been documented to protect people from Parkinson's disease and other neurodegenerative diseases [ 54 ].…”