Modeling of particle capture in high gradient magnetic separation: A review

Zheng, Xiayu; Xue, Zixing; Wang, Yuhua; Zhu, Guangli; Lu, Dongfang; Li, Xudong

doi:10.1016/j.powtec.2019.04.048

Cited by 33 publications

(12 citation statements)

References 77 publications

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“…In high‐gradient magnetic separation, the magnetic field distribution and thus, the gradient of the magnetic field is significantly influenced by the material of the filter matrix as well as its structure, arrangement, and size [32–35]. Since the matrix normally comes into contact with the suspension, it should have chemical resistance and corrosion resistance, as well as meeting leachables and extractables (L&E) criteria in biopharmaceutical applications.…”

Section: Introductionmentioning

confidence: 99%

Development of a 3D‐printed single‐use separation chamber for use in mRNA‐based vaccine production with magnetic microparticles

Wommer

Meiers

Kockler

et al. 2021

Engineering in Life Sciences

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Laboratory protocols using magnetic beads have gained importance in the purification of mRNA for vaccines. Here, the produced mRNA hybridizes specifically to oligo(dT)‐functionalized magnetic beads after cell lysis. The mRNA‐loaded magnetic beads can be selectively separated using a magnet. Subsequently, impurities are removed by washing steps and the mRNA is eluted. Magnetic separation is utilized in each step, using different buffers such as the lysis/binding buffer. To reduce the time required for purification of larger amounts of mRNA vaccine for clinical trials, high‐gradient magnetic separation (HGMS) is suitable. Thereby, magnetic beads are selectively retained in a flow‐through separation chamber. To meet the requirements of biopharmaceutical production, a disposable HGMS separation chamber with a certified material (United States Pharmacopeia Class VI) was developed which can be manufactured using 3D printing. Due to the special design, the filter matrix itself is not in contact with the product. The separation chamber was tested with suspensions of oligo(dT)‐functionalized Dynabeads MyOne loaded with synthetic mRNA. At a concentration of cB = 1.6–2.1 g·L–1 in lysis/binding buffer, these 1 μm magnetic particles are retained to more than 99.39% at volumetric flows of up to 150 mL·min–1 with the developed SU‐HGMS separation chamber. When using the separation chamber with volumetric flow rates below 50 mL·min–1, the retained particle mass is even more than 99.99%.

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Section: Introductionmentioning

confidence: 99%

Development of a 3D‐printed single‐use separation chamber for use in mRNA‐based vaccine production with magnetic microparticles

Wommer

Meiers

Kockler

et al. 2021

Engineering in Life Sciences

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show abstract

“…Different methods are used for the calculation of magnetic field gradients in Electromagnetic Field Theory [31]. However, the formulas obtained by methods based on the calculation of the scalar magnetic field potential, commonly used in classical electromagnetic field theory, are complicated and not appropriate for the practical calculations in separation practice [9], [11], [17], [32]. This prevents both the progression of biomagnetic separation theory and the expansion of its applicability in different areas.…”

Section: Introductionmentioning

confidence: 99%

Measurement and Modelling of Gradient Magnetic Fields for Bio-Chemical Separation Processes

Bilgili

Abbasov

Baran

2021

Int. J. Eng. Sci. Tech

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Separation processes are widely used in chemical and biotechnical processes. Especially biomagnetic separation is an important issue among effective separation processes to separate the magnetic micron and submicron particles. It is necessary to establish and determine a high magnetic field or field gradient in the separation cell. However, it is not easy to determine the magnetic field gradient in the working region for different separation in practice. The reason for these difficulties is that the magnetic cells used in biochemical separation have different geometries and there are no simple and useful systems to easily measure these magnetic fields. Two main objectives are aimed in this study. First, a simple measuring device design can measure gradient magnetic fields with high precision of about 0,01mm and, secondly, obtain simple empirical expressions for the magnetic field. A magnetometer with Hall probes that works with the 3D printer principle was designed and tested to measure the magnetic field. Magnetic field changes were measured according to the surface coordinates on the measurement platform or measuring cell. Numerous experimental measurements of gradient magnetic fields generated by permanent magnets have been taken. The results obtained from the studies and results from the proposed empirical models were compared.

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“…In achieving productive separation, the presence of steel wool combined with high magnetic strength is significantly important to achieve a successful HGMS application. Fibrous steel wool has a large surface area, which homogenizes with a uniform magnetic field that promotes a higher magnetic gradient (Zheng et al, 2019). Hence, weak magnetic particles are easily attracted to higher magnetic force and simultaneously trapped in the steel wool (Ebner et al, 1997;Mariani et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Optimization and Modelling of Turbidity Removal of Sewage using High-Gradient Magnetic Separation (HGMS) by Response Surface Methodology (RSM)

Supian¹,

Sohaili²,

Zon³

2020

JST

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Endless industrial development and growing society occasionally create an enormous volume of wastewater, which leads to some issues on wastewater treatment. Existing conventional screening processes have various limitations and drawbacks. Therefore, this study investigated the use of a combination of non-corrosive stainless steel wool and a permanent magnet to increase magnetic gradient, hence reducing suspended matter in sewage through turbidity test. An approach for optimizing the reduction of suspended matter through turbidity analysis was conducted using central composite design (CCD) under response surface methodology (RSM). Three critical independent variables, such as magnet strength, circulation time, and steel wool, and turbidity removal as the response, were further studied to analyze their interaction effects. As a result, an optimal value of turbidity removal was found at 90.3% under the specified optimum conditions of magnet strength of 245 mT, 116 g of non-corrosive stainless steel wool, and 16 h of circulation time. Statistical analysis had shown that the magnet strength, circulation time, and steel wool significantly affected the turbidity removal performance. Furthermore, design of experiment was significantly verified by a small range of error between predicted and actual data. Consequently, a higher gradient of magnetic separation was proven to effectively remove suspended matter using inexpensive non-corrosive stainless steel wool without using magnetic adsorbent. Thus, the suggested approach was found to be cost-effective and environmentally friendly for sewage treatment.

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Modeling of particle capture in high gradient magnetic separation: A review

Cited by 33 publications

References 77 publications

Development of a 3D‐printed single‐use separation chamber for use in mRNA‐based vaccine production with magnetic microparticles

Development of a 3D‐printed single‐use separation chamber for use in mRNA‐based vaccine production with magnetic microparticles

Measurement and Modelling of Gradient Magnetic Fields for Bio-Chemical Separation Processes

Optimization and Modelling of Turbidity Removal of Sewage using High-Gradient Magnetic Separation (HGMS) by Response Surface Methodology (RSM)

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