Modeling of Luminescence Quenching-Based Sensors: Comparison of Multisite and Nonlinear Gas Solubility Models

Demas, J. N.; DeGraff, Β. A.; Xu, Wei

doi:10.1021/ac00104a012

Cited by 259 publications

(194 citation statements)

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“…To determine the dissolved oxygen, the measured phase shift of the oxygen signal was related to the oxygen concentration using a modified Stern -Volmer equation (Carraway et al, 1991;Demas et al, 1995). An 11-point calibration between 0% and 100% oxygen was carried out to confirm the validity of the equation and to calculate a Stern -Volmer constant.…”

Section: Methodsmentioning

confidence: 99%

Membrane‐aerated microbioreactor for high‐throughput bioprocessing

Zanzotto

Szita

Boccazzi

et al. 2004

Biotech & Bioengineering

188

193

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A microbioreactor with a volume of microliters is fabricated out of poly(dimethylsiloxane) (PDMS) and glass. Aeration of microbial cultures is through a gas-permeable PDMS membrane. Sensors are integrated for on-line measurement of optical density (OD), dissolved oxygen (DO), and pH. All three parameter measurements are based on optical methods. Optical density is monitored via transmittance measurements through the well of the microbioreactor while dissolved oxygen and pH are measured using fluorescence lifetime-based sensors incorporated into the body of the microbioreactor. Bacterial fermentations carried out in the microbioreactor under well-defined conditions are compared to results obtained in a 500-mL bench-scale bioreactor. It is shown that the behavior of the bacteria in the microbioreactor is similar to that in the larger bioreactor. This similarity includes growth kinetics, dissolved oxygen profile within the vessel over time, pH profile over time, final number of cells, and cell morphology. Results from off-line analysis of the medium to examine organic acid production and substrate utilization are presented. By changing the gaseous environmental conditions, it is demonstrated that oxygen levels within the microbioreactor can be manipulated. Furthermore, it is demonstrated that the sensitivity and reproducibility of the microbioreactor system are such that statistically significant differences in the time evolution of the OD, DO, and pH can be used to distinguish between different physiological states. Finally, modeling of the transient oxygen transfer within the microbioreactor based on observed and predicted growth kinetics is used to quantitatively characterize oxygen depletion in the system.

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Section: Methodsmentioning

confidence: 99%

Membrane‐aerated microbioreactor for high‐throughput bioprocessing

Zanzotto

Szita

Boccazzi

et al. 2004

Biotech & Bioengineering

188

193

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“…It decreases the intensity of the foil's fluorescence emission and decreases the lifetime of the excited state of the luminophores. The linear Stern-Volmer equation relates lifetime to oxygen and is derived using bimolecular quenching kinetics to be: (1) where ] is the Henry's Law oxygen solubility coefficient for the foil (Demas et al 1995). Fundamentally, k Q reflects the efficiency of quenching or accessibility of the luminophores to oxygen and can be written as:…”

Section: Linear Stern-volmer Relationshipmentioning

confidence: 99%

A calibration equation for oxygen optodes based on physical properties of the sensing foil

McNeil

D’Asaro

2014

Limnology & Ocean Methods

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We present a new physically based calibration equation for Aanderaa Inc. oxygen sensing optodes. We use the two site fluorescence quenching model of Demas et al. (1995) to describe the nonlinear Stern-Volmer response of the optode foil to oxygen partial pressure. Seven (minimally six) coefficients quantify foil response to oxygen and temperature; another quantifies response to hydrostatic pressure. These eight coefficients are related, theoretically, to basic physical properties of the foil. The equation provides a framework to study causes of variability and drift in optodes and to develop better quality control and handling procedures. We tested the equation using factory calibrations of 24 optode foils. When accurate multi-point calibration data are unavailable, two additional coefficients empirically correct the usually large differences observed between factory foil calibrations and post-factory laboratory/field calibrations; we cannot eliminate this major cause of uncertainty in optode calibrations. Excluding two potentially anomalous foils, the calibration equation fits 13 similarly calibrated foils, totaling 455 calibration points over 3 -40°C to -0.57 ± 1.48 mbar. Analysis of the resulting best fit coefficients reveals an underlying variability in optodes associated with variability in site 2 and site 1 Stern-Volmer coefficients of 32% and 20%, respectively. The fraction of unquenched fluorophores responding with the more accessible site 1 quenching characteristics varies by only 3%. The equation fits multi-point data for two optodes within manufacturer's specifications, the greater of ± 2.5 μmol . kg -1 and ± 1.5%. Detailed measurements of calibration changes over time will be required to understand the causes of optode drift.

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“…The ratio I 0 /I 100 has been used as a sensitivity of the sensing film; the sensor having the ratio I 0 /I 100 more than 3.0 is a suitable oxygen sensing device. 33 The I 0 /I 100 of PtOEP dispersed in the poly(TMSP), polystyrene and poly(DMS) films were 225, 4.5, and 5.5, respectively. On the other hand, the I 0 /I 100 of PdOEP dispersed in the poly(TMSP), polystyrene and poly(DMS) films were 121, 46.0, 50.1, respectively.…”

Section: Luminescence Spectrum Change Of Oep Metal Complex Dispersed mentioning

confidence: 97%

An Optical Sensing Material for Trace Analysis of Oxygen. Metalloporphyrin Dispersed in Poly(1-trimethylsilyl-1-propyne) Film

Amao

Okura

Shinohara

et al. 2002

Polym J

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ABSTRACT:A highly gas permeable polymer, poly(1-trimethylsilyl-1-propyne) (poly(TMSP)), was applied as a matrix of the optical oxygen sensor using the oxygen-induced luminescence quenching of octaethylporphyrin (OEP) platinum (PtOEP) or palladium complex (PdOEP). OEP complex was homogeneously dispersed in poly(TMSP) to give a mechanical tough film with thickness of 10 µm. The luminescence intensity of OEP complex dispersed in the poly(TMSP) films drastically decreased with an increase in oxygen concentration. The oxygen sensitivity of the film (I 0 /I 100 ) was very high and estimated to be 225 for PtOEP and 121 for PdOEP, respectively. The Stern-Volmer constants of PtOEP and PdOEP dispersed in poly(TMSP) films are estimated to be 6.6 and 17%−1 , respectively. These results indicate that OEP metal complex dispersed in the poly(TMSP) films are novel optical sensing material for trace analysis of oxygen. KEY WORDS Poly(1-trimethylsilyl-1-propyne) / Optical Oxygen Sensor / Metalloporphyrin / Luminescence Quenching / Trace Analysis for Oxygen / The measurement of oxygen concentration is important in various fields of chemical, clinical analysis and environmental monitoring. 1-3 The sensing system for oxygen concentration is classified into titration, 4 amperometry, 5 chemiluminescence, 6 thermoluminescence 7 and colorinmetry. 8 Among these systems, the most popular method is the amperometric method using an oxygen electrode, 5 in which the rate of oxygen diffusion to the cathode is measured. This system, however, is limited because of the stability of the electrode surface. Recently, a variety of devices and sensors based on luminescence quenching of organic dyes were developed to measure oxygen concentrations or partial pressures. Many studies on optical oxygen sensors are used organic dyes, such as polycyclic aromatic hydrocarbons (pyrene and its derivatives, quinoline and phenanthrene), 9-15 transition metal complexes (ruthenium, 16-21 osmium 22 or rhenium-polypyridine complexes 23 ), and metalloporphyrins, 24-26 dispersed in polymers (silicone polymer, polystyrene, etc.). Among these organic dyes, platinum and palladium porphyrins show strong phosphorescence at room temperature. 27 Especially, platinum and palladium octaethylporphyrin (PtOEP and PdOEP) display strong room-temperature phosphorescence with high quantum yield (φ P < 0.5) and long lifetime (ca. 100 µs for PtOEP and ca. 770 µs for PdOEP). 27 Some optical oxygen sensors based on the phosphorescence quenching of PtOEP or PdOEP have been developed by dispersing it in polymer † To whom correspendence should be addressed.

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Modeling of Luminescence Quenching-Based Sensors: Comparison of Multisite and Nonlinear Gas Solubility Models

Cited by 259 publications

References 0 publications

Membrane‐aerated microbioreactor for high‐throughput bioprocessing

Membrane‐aerated microbioreactor for high‐throughput bioprocessing

A calibration equation for oxygen optodes based on physical properties of the sensing foil

An Optical Sensing Material for Trace Analysis of Oxygen. Metalloporphyrin Dispersed in Poly(1-trimethylsilyl-1-propyne) Film

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