Modeling of Anaerobic Digestion Considering Biowaste by Syntrophic Propionate‐Oxidizing Bacteria

Abstract

Die anaerobe Vergärung von Bioabfällen erzeugt nachhaltig Bioenergie und verringert Umweltbelastungen. Die acetogene Gärphase wurde mit dem Anaerobic Digestion Model No. 1 (ADM1) modelliert, das um drei Gattungen Propionat-oxidierender Bakterien und zwei Varianten des Propionatabbaus, den Methyl-Malonyl-CoA-und C6-Dismutase-Weg, erweitert wurde. Die kinetischen Parameter für die anaerobe Propionat-Oxidation wurden mit definierten Trikulturen aus den drei Propionat-oxidierenden Gattungen mit hydrogenotrophen und acetotrophen methanogenen Archaea bestimmt und in das ADM1 implementiert, woraus das verbesserte Modell ADM1xpro resultierte.Schlagwörter: Biogas, Mathematische Modellierung, Nassvergärung von Bioabfällen, Propionat-oxidierende Bakterien Anaerobic digestion of biowaste allows a sustainable production of bioenergy and reduces environmental burden. The anaerobic digestion phase was modeled with Anaerobic Digestion Model No. 1 (ADM1), which was extended to include three genera of propionate-oxidizing bacteria and two routes of propionate degradation, namely methyl-malonyl CoA and C6-dismutation pathway. Kinetic parameters for anaerobic propionate oxidation were determined with defined tricultures of the three propionate-oxidizing genera together with hydrogenotrophic and acetotrophic methanogens and implemented into ADM1, resulting in the improved model ADM1xpro.

Validation and Uncertainty Assessment of a Software‐Integrated Workflow for pH Calculations

“…Especially in anaerobic fermentation, the importance of pH extends beyond water treatment and supply, as high added-value products from various biomasses, including organic waste, can be generated [7]. Carboxylic acids, such as formic acid, acetic acid, propanoic acid, butanoic acid, and others, are among the products generated during anaerobic fermentation [3,[7][8][9][10]. These carboxylic acids are crucial building blocks for the production of other valuable compounds such as alcohols and aldehydes.…”