Modeling Myeloid Malignancies Using Zebrafish

Potts, Kathryn; Bowman, Teresa V.

doi:10.3389/fonc.2017.00297

Cited by 17 publications

(15 citation statements)

References 140 publications

(150 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zebrafish has been extensively used for modeling human hematopoietic disease, including anemia, thrombocytopenia, bone marrow failure syndromes, leukemia, and lymphoma ( Taylor and Zon, 2011 ; Kwan and North, 2017 ; Potts and Bowman, 2017 ; Gore et al, 2018 ). The first transplantable leukemia modeled in zebrafish was T-cell acute lymphoblastic leukemia (T-ALL), which was induced by T cell-specific c-Myc overexpression ( Langenau et al, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

Bloody Zebrafish: Novel Methods in Normal and Malignant Hematopoiesis

Pater

Trompouki

2018

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Hematopoiesis is an optimal system for studying stem cell maintenance and lineage differentiation under physiological and pathological conditions. In vertebrate organisms, billions of differentiated hematopoietic cells need to be continuously produced to replenish the blood cell pool. Disruptions in this process have immediate consequences for oxygen transport, responses against pathogens, maintenance of hemostasis and vascular integrity. Zebrafish is a widely used and well-established model for studying the hematopoietic system. Several new hematopoietic regulators were identified in genetic and chemical screens using the zebrafish model. Moreover, zebrafish enables in vivo imaging of hematopoietic stem cell generation and differentiation during embryogenesis, and adulthood. Finally, zebrafish has been used to model hematopoietic diseases. Recent technological advances in single-cell transcriptome analysis, epigenetic regulation, proteomics, metabolomics, and processing of large data sets promise to transform the current understanding of normal, abnormal, and malignant hematopoiesis. In this perspective, we discuss how the zebrafish model has proven beneficial for studying physiological and pathological hematopoiesis and how these novel technologies are transforming the field.

show abstract

Section: Introductionmentioning

confidence: 99%

Bloody Zebrafish: Novel Methods in Normal and Malignant Hematopoiesis

Pater

Trompouki

2018

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Therefore, it is of high interest to have reliable animal models which would allow to better understand the molecular pathogenesis of hematopoietic malignancies. There are classic models of myeloid leukemia in zebrafish, based on chromosomal rearrangements known from humans as well as murine models, based on oncogene mutations or oncogene overexpression [67]. The fusion of AML1 with ETO is one of the most commonly found fusions in acute myeloid leukemia (AML).…”

Section: Genetic Models Of Cancer In Zebrafishmentioning

confidence: 99%

“…A representation of cancer types discussed in this section is depicted in Figure 1 with individual sites of tumors within an adult zebrafish. Further models of tumorigenesis [8,19,76] and leukemia in zebrafish are compiled in other review papers [65,67,77,78,79].…”

Section: Genetic Models Of Cancer In Zebrafishmentioning

confidence: 99%

Zebrafish Models of Cancer—New Insights on Modeling Human Cancer in a Non-Mammalian Vertebrate

Hason

Bartůněk

2019

Genes

143

114

View full text Add to dashboard Cite

Zebrafish (Danio rerio) is a valuable non-mammalian vertebrate model widely used to study development and disease, including more recently cancer. The evolutionary conservation of cancer-related programs between human and zebrafish is striking and allows extrapolation of research outcomes obtained in fish back to humans. Zebrafish has gained attention as a robust model for cancer research mainly because of its high fecundity, cost-effective maintenance, dynamic visualization of tumor growth in vivo, and the possibility of chemical screening in large numbers of animals at reasonable costs. Novel approaches in modeling tumor growth, such as using transgene electroporation in adult zebrafish, could improve our knowledge about the spatial and temporal control of cancer formation and progression in vivo. Looking at genetic as well as epigenetic alterations could be important to explain the pathogenesis of a disease as complex as cancer. In this review, we highlight classic genetic and transplantation models of cancer in zebrafish as well as provide new insights on advances in cancer modeling. Recent progress in zebrafish xenotransplantation studies and drug screening has shown that zebrafish is a reliable model to study human cancer and could be suitable for evaluating patient-derived xenograft cell invasiveness. Rapid, large-scale evaluation of in vivo drug responses and kinetics in zebrafish could undoubtedly lead to new applications in personalized medicine and combination therapy. For all of the above-mentioned reasons, zebrafish is approaching a future of being a pre-clinical cancer model, alongside the mouse. However, the mouse will continue to be valuable in the last steps of pre-clinical drug screening, mostly because of the highly conserved mammalian genome and biological processes.

show abstract

“…Hematopoietic stem cells (HSCs) produce all blood cell types throughout life due to their capacity for self-renewal and differentiation [ 1 , 2 ]. Any disruption of this process can lead to abnormal expansion of cellular clones, which may lead to hematologic malignancies such as acute myeloid leukemia (AML) [ 2 , 3 , 4 , 5 ]. AML is a heterogeneous disease with extreme proliferation of myeloblasts (>20%) in the bone marrow (BM) [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

A Role for the Bone Marrow Microenvironment in Drug Resistance of Acute Myeloid Leukemia

Bolandi

Pakjoo

Beigi

et al. 2021

Cells

View full text Add to dashboard Cite

Acute myeloid leukemia (AML) is a heterogeneous disease with a poor prognosis and remarkable resistance to chemotherapeutic agents. Understanding resistance mechanisms against currently available drugs helps to recognize the therapeutic obstacles. Various mechanisms of resistance to chemotherapy or targeted inhibitors have been described for AML cells, including a role for the bone marrow niche in both the initiation and persistence of the disease, and in drug resistance of the leukemic stem cell (LSC) population. The BM niche supports LSC survival through direct and indirect interactions among the stromal cells, hematopoietic stem/progenitor cells, and leukemic cells. Additionally, the BM niche mediates changes in metabolic and signal pathway activation due to the acquisition of new mutations or selection and expansion of a minor clone. This review briefly discusses the role of the BM microenvironment and metabolic pathways in resistance to therapy, as discovered through AML clinical studies or cell line and animal models.

show abstract

Modeling Myeloid Malignancies Using Zebrafish

Cited by 17 publications

References 140 publications

Bloody Zebrafish: Novel Methods in Normal and Malignant Hematopoiesis

Bloody Zebrafish: Novel Methods in Normal and Malignant Hematopoiesis

Zebrafish Models of Cancer—New Insights on Modeling Human Cancer in a Non-Mammalian Vertebrate

A Role for the Bone Marrow Microenvironment in Drug Resistance of Acute Myeloid Leukemia

Contact Info

Product

Resources

About