Modeling infectious diseases and host-microbe interactions in gastrointestinal organoids

Bartfeld, Sina

doi:10.1016/j.ydbio.2016.09.014

Cited by 91 publications

(64 citation statements)

References 78 publications

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“…Historically, this included models engineered with different technologies using cell lines, stem cells, primary cells, or tissue explants either embedded in or cultured on top of ECM scaffolds that allow cells to self-assemble into 3-D structures (8,12,143,145,146,169,171,(221)(222)(223)(224)(225)(226)(227)(228)(229). Advances in stem cell biology led to a recent terminology shift to more specifically define organoids as 3-D models derived from stem cells, progenitor cells, or primary explants (222,(230)(231)(232)(233)(234)(235)(236)(237)(238). Here we focus on 3-D models cultured within a 3-D ECM that fit this definition.…”

Section: Modeling the Microenvironment: 3-d Models For Infectious Dismentioning

confidence: 99%

“…A range of different organoid models have been established, including small and large intestine (229,230,232,234,, lung (269)(270)(271)(272)(273)(274), stomach (275)(276)(277)(278)(279)(280)(281)(282), breast (55,283,284), brain (285)(286)(287), liver (222,288,289), pancreas (222,290,291), gallbladder (292), eye (293), kidney (294), prostate (222,295,296) and reproductive tract (297,298), among others. Relative to monolayers, these models more closely mimic endogenous tissues, including organization and spontaneous differentiation of multiple cell types into physiologically relevant 3-D structures (Fig.…”

Section: Modeling the Microenvironment: 3-d Models For Infectious Dismentioning

confidence: 99%

“…Model infection can be accomplished by (i) addition of pathogen directly to the media (basal side), (ii) microinjection into the lumen (Fig. 1B, panel b), (iii) shearing of models into fragments followed by pathogen addition, and (iv) complete disruption of 3-D models into flat monolayers followed by pathogen addition (230,237). Consideration of the normal infection route is critical.…”

Section: Modeling the Microenvironment: 3-d Models For Infectious Dismentioning

confidence: 99%

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Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age

et al. 2018

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Tissues and organs provide the structural and biochemical landscapes upon which microbial pathogens and commensals function to regulate health and disease. While flat two-dimensional (2-D) monolayers composed of a single cell type have provided important insight into understanding host-pathogen interactions and infectious disease mechanisms, these reductionist models lack many essential features present in the native host microenvironment that are known to regulate infection, including three-dimensional (3-D) architecture, multicellular complexity, commensal microbiota, gas exchange and nutrient gradients, and physiologically relevant biomechanical forces (e.g., fluid shear, stretch, compression). A major challenge in tissue engineering for infectious disease research is recreating this dynamic 3-D microenvironment (biological, chemical, and physical/mechanical) to more accurately model the initiation and progression of host-pathogen interactions in the laboratory. Here we review selected 3-D models of human intestinal mucosa, which represent a major portal of entry for infectious pathogens and an important niche for commensal microbiota. We highlight seminal studies that have used these models to interrogate host-pathogen interactions and infectious disease mechanisms, and we present this literature in the appropriate historical context. Models discussed include 3-D organotypic cultures engineered in the rotating wall vessel (RWV) bioreactor, extracellular matrix (ECM)-embedded/organoid models, and organ-on-a-chip (OAC) models. Collectively, these technologies provide a more physiologically relevant and predictive framework for investigating infectious disease mechanisms and antimicrobial therapies at the intersection of the host, microbe, and their local microenvironments.

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Section: Modeling the Microenvironment: 3-d Models For Infectious Dismentioning

confidence: 99%

Section: Modeling the Microenvironment: 3-d Models For Infectious Dismentioning

confidence: 99%

Section: Modeling the Microenvironment: 3-d Models For Infectious Dismentioning

confidence: 99%

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Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age

et al. 2018

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“…However, what we should emphasize is that the organotypic culture systems for various types of intestinal cells are increasingly becoming an important tool for multiple disciplines in intestinal epithelial research [8][9][10][11][12].…”

Section: Intestinal Organoidsmentioning

confidence: 99%

“…Intestinal epithelia grown in these 3D organoid systems recapitulate many aspects of their structure and function in in vivo settings. Thus, these organoid systems are gaining much attention as the organotypic model to study tissue homeostasis, developmental processes, host-microbe interactions, and the mechanisms of the diseases in the intestine [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Intestinal stem cell transplantation

Nakamura

Watanabe

2016

J Gastroenterol

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Organoid technologies to expand intestinal epithelial cells are gaining increasing attention as a useful tool to investigate many aspects of intestinal epithelial biology and pathology. One important application of organoid systems would be to use intestinal epithelial cells expanded in culture for following transplantation experiments. In this article, we present a brief overview of the studies that have succeeded in generating new epithelial tissues in the surface of native intestines in mice by organoid transplantation. We also discuss possible applications of this experimental approach in basic research on the intestinal epithelium as well as in regenerative medicine for various types of intestinal diseases in humans.

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Norovirus

Iturriza‐Gómara

Harris

2017

Encyclopedia of Life Sciences

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Norovirus infections are the leading cause of acute gastroenteritis globally. Despite the increasing recognition of norovirus infections in various animal species, there is to date no evidence of an animal reservoir for human norovirus, and transmission of norovirus is primarily person‐to‐person. Contaminated food, water and environment act as important amplifiers for infection. Human noroviruses are very diverse; however, one genotype GII‐4 predominates globally and is responsible for nearly half of all the infections and for a higher proportion of the outbreaks. Recently, in vitro systems for the propagation of human noroviruses have been developed, and there is an expectation that in the coming years, these will provide new insights into the molecular biology of human noroviruses and a better understanding of factors that determine susceptibility to and protection form infection. Host genetic factors and comorbidities in addition to potentially modifiable factors such as microbiome and use of certain treatment and drugs are increasingly recognised as important drivers of susceptibility to infection. Key Concepts Norovirus is the leading cause of acute gastroenteritis in humans. Transmission of norovirus is predominantly person‐to‐person and is greatly amplified via contaminated food, water and environment. Glycans act as attachment factors that mediate viral infection and cell entry and potentially drive host restriction. New in vitro systems have recently been developed for human norovirus replication: B cell‐ and stem cell‐derived enteroids. Virus diversity and host genetic and acquired factors are important drivers of susceptibility to infection. The host microbiome has been recognised as an important factor for susceptibility to norovirus infection and clearance.

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Modeling infectious diseases and host-microbe interactions in gastrointestinal organoids

Abstract: Cite this article as: Sina Bartfeld, Modeling infectious diseases and host-microbe interactions in gastrointestinal organoids, Developmental Biology, http://dx

Cited by 91 publications

References 78 publications

Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age

Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age

Intestinal stem cell transplantation

Norovirus

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