Modeling heat losses in microfluidic devices: The case of static chamber devices for DNA amplification

Papadopoulos, Vassilios; Kefala, Ioanna N.; Kaprou, Georgia D.; Tserepi, Angeliki; Kokkoris, George

doi:10.1016/j.ijheatmasstransfer.2021.122011

Cited by 4 publications

(1 citation statement)

References 55 publications

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“…With the development of micro-electro-mechanical systems (MEMS), while numbers of research on the measurement and calibration of microfluidics temperature have been conducted [ 8 , 9 , 10 ], a unified and systematic theory is still ambiguous. Therefore, the study on the microfluidic heat transfer characteristics and temperature calibration is kept hot [ 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Microfluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer

Meng

et al. 2022

Micromachines

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Microfluidic devices offer excellent heat transfer, enabling the biochemical reactions to be more efficient. However, the precision of temperature sensing and control of microfluids is limited by the size effect. Here in this work, the relationship between the microfluids and the glass substrate of a typical microfluidic device is investigated. With an intelligent structure design and liquid metal, we demonstrated that a millimeter-scale industrial temperature sensor could be utilized for temperature sensing of micro-scale fluids. We proposed a heat transfer model based on this design, where the local correlations between the macro-scale temperature sensor and the micro-scale fluids were investigated. As a demonstration, a set of temperature-sensitive nucleic acid amplification tests were taken to show the precision of temperature control for micro-scale reagents. Comparations of theoretical and experimental data further verify the effectiveness of our heat transfer model. With the presented compensation approach, the slight fluorescent intensity changes caused by isothermal amplification polymerase chain reaction (PCR) temperature could be distinguished. For instance, the probability distribution plots of fluorescent intensity are significant from each other, even if the amplification temperature has a difference of 1 °C. Thus, this method may serve as a universal approach for micro–macro interface sensing and is helpful beyond microfluidic applications.

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Section: Introductionmentioning

confidence: 99%

Microfluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer

Meng

et al. 2022

Micromachines

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Numerical simulation, fabrication, and characterization of a heating system for integration into an Organ-on-a-Chip

Ferreira,

Azevedo,

Sousa

et al. 2023

Sensors and Actuators A: Physical

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Analysis of heat generation and transfer in droplet-based acoustofluidic by considering the attenuation effect

Huang,

Zhou,

et al. 2024

Physics of Fluids

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The droplet-based acoustic microfluidic system has recently demonstrated excellent performance and application potential in biological micro/nanoparticle manipulation, chemical synthesis, and materials science. However, in those applications with obvious temperature response, the heat generation of droplets under the action of surface acoustic waves (SAW) is ignored usually, which highlights the need to analyze the process of SAW heating droplets to guide temperature control. Here, based on the system with relative acoustic field bias arrangement, the influence of SAW attenuation on the distribution of heat sources was studied by constructing a coupled model of heat source heating and fluid heat transfer. The role of SAW attenuation effect on the initial stage of droplet heat generation was analyzed through experiments and simulations, and the mechanism of local heat sources on droplet heat generation was clarified. The temperature field of droplets was determined by the local heat source caused by SAW attenuation and the convective heat transfer generated by the acoustic streaming effect. The correspondence between input power and temperature field was also analyzed, to clarify the scheme for adjusting droplet temperature. This work clarifies the temperature control mechanism of this acoustic systems and enables applications such as biochemical reactions or DNA (deoxyribonucleic acid) amplification.

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Modeling heat losses in microfluidic devices: The case of static chamber devices for DNA amplification

Cited by 4 publications

References 55 publications

Microfluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer

Microfluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer

Numerical simulation, fabrication, and characterization of a heating system for integration into an Organ-on-a-Chip

Analysis of heat generation and transfer in droplet-based acoustofluidic by considering the attenuation effect

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