Modeling evolutionary growth of a microRNA-mediated regulation system

Akita, Tetsuya; Takuno, Shohei; Innan, Hideki

doi:10.1016/j.jtbi.2012.07.011

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…MicroRNAs (miRNAs) are evolutionary conserved small RNAs in eukaryotic organisms, which are involved in post-transcriptional regulation (8). Various studies have found that the abnormal expression of miRNAs is associated with numerous human diseases, such as lung cancer, renal cell carcinoma and inflammation (9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

miR-155 is upregulated in patients with active tuberculosis and inhibits apoptosis of monocytes by targeting FOXO3

Huang

Jiao

et al. 2015

Molecular Medicine Reports

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The aim of the present study was to investigate the association between microRNA (miR)-155 and apoptosis of monocytes infected by Mycobacterium tuberculosis, to examine the effect of forkhead box O3 (FOXO3) on miR‑155. The present study analysed the apoptosis of CD14+ in the peripheral blood of patients with active tuberculosis, disposed the THP‑1 human monocytic cell line by BCG and examined the expression of miR‑155. Furthermore, the expression of FOXO3 in THP‑1 cells was determined, and wild- and mutant-type luciferase reporter plasmids containing FOXO3 3'‑untranslated regions (UTRs) were constructed to analyse the expression of luciferase. Finally, an over‑expression plasmid was constructed, and THP-1 cells were transfected with control miRNA, miR‑155 and the plasmid, which revealed that miR‑155 inhibited the apoptosis of THP‑1 cells. miR‑155 in the THP‑1 cells infected by BCG was upregulated and apoptosis also increased. However, the apoptosis declined when the cells were transfected with the control miRNA and miR‑155. Folllowing transfection with miR‑155, the expression of FOXO3 decreased. Transfection with miR‑155 and the FOXO3 3'-UTRs significantly reduced luciferase, and overexpression of FOXO3 reversed the inhibitory role of miR‑155. From these results, it was concluded that mycobacteria can improve the level of miR‑155, while BCG can induce apoptosis in THP‑1 cells. The results suggested FOXO3 is a downstream target gene of miR‑155, which combines 3'-UTRs to inhibit the expression of FOXO3.

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Section: Introductionmentioning

confidence: 99%

miR-155 is upregulated in patients with active tuberculosis and inhibits apoptosis of monocytes by targeting FOXO3

Huang

Jiao

et al. 2015

Molecular Medicine Reports

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“…New routes for ncRNAs evolution (i.e., gene duplication events) 43 per se can be discovered through probable ncRNAs mediating genetic transfer between the organellar genomes and the nuclear genome. Based on their universal character, ncRNAs could probably be applied as an indirect in vivo probing of the different sub-compartmental pH and redox potentials, since a detailed quantification is still missing in (plant) eukarya, due to the membrane barriers.…”

Section: Discussionmentioning

confidence: 99%

Non-coding RNAs’ partitioning in the evolution of photosynthetic organisms via energy transduction and redox signaling

Kotakis

2015

RNA Biology

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Ars longa, vita brevis -Hippocrates Chloroplasts and mitochondria are genetically semi-autonomous organelles inside the plant cell. These constructions formed after endosymbiosis and keep evolving throughout the history of life. Experimental evidence is provided for active non-coding RNAs (ncRNAs) in these prokaryote-like structures, and a possible functional imprinting on cellular electrophysiology by those RNA entities is described. Furthermore, updated knowledge on RNA metabolism of organellar genomes uncovers novel inter-communication bridges with the nucleus. This class of RNA molecules is considered as a unique ontogeny which transforms their biological role as a genetic rheostat into a synchronous biochemical one that can affect the energetic charge and redox homeostasis inside cells. A hypothesis is proposed where such modulation by non-coding RNAs is integrated with genetic signals regulating gene transfer. The implications of this working hypothesis are discussed, with particular reference to ncRNAs involvement in the organellar and nuclear genomes evolution since their integrity is functionally coupled with redox signals in photosynthetic organisms.

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Modeling evolutionary growth of a microRNA-mediated regulation system

Cited by 2 publications

References 32 publications

miR-155 is upregulated in patients with active tuberculosis and inhibits apoptosis of monocytes by targeting FOXO3

miR-155 is upregulated in patients with active tuberculosis and inhibits apoptosis of monocytes by targeting FOXO3

Non-coding RNAs’ partitioning in the evolution of photosynthetic organisms via energy transduction and redox signaling

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