Modeling Epiblast Shape in Implanting Mammalian Embryos

Dokmegang, Joel

doi:10.1007/978-1-0716-2281-0_20

Cited by 3 publications

(1 citation statement)

References 58 publications

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“…This is especially true when it comes to morphogenesis Dalmasso et al (2021); Romeo et al (2021); Mitchell and Cislo (2022 ). The processes involved in development are inherently multiscale, both in the spatial and temporal domains, and may interact or overlap Godard and Heisenberg (2019); Dokmegang et al (2021); Dokmegang (2022 ). As it is the case with several species Godard and Heisenberg (2019 ), ascidian gastrulation is a playground featuring important displays of cellular divisions and tissue mechanics, most notably endoderm invagination Lemaire (2009 ).…”

Section: Introductionmentioning

confidence: 99%

Spectral decomposition unlocks ascidian morphogenesis

Dokmegang,

Faure,

Lemaire

et al. 2023

Preprint

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Describing morphogenesis generally consists in aggregating the multiple high resolution spatiotemporal processes involved into repeatable low dimensional morphological processes consistent across individuals of the same species or group. In order to achieve this goal, biologists often have to submit movies issued from live imaging of developing embryos either to a qualitative analysis or to basic statistical analysis. These approaches, however, present noticeable drawbacks, as they can be time consuming, hence unfit for scale, and often lack standardisation and a firm foundation. In this work, we leverage the power of a continuum mechanics approach and flexibility of spectral decompositions to propose a standardised framework for automatic detection and timing of morphological processes. First, we quantify whole-embryo scale shape changes in developing ascidian embryos by statistically estimating the strain-rate tensor field of its time-evolving surface without the requirement of cellular segmentation and tracking. We then apply to this data spectral decomposition in space using spherical harmonics and and in time using wavelets transforms. These transformations result in the identification of the principal dynamical modes of ascidian embryogenesis and the automatic unveiling of its blueprint in the form of spectograms that tell the story of development in ascidian embryos.

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Section: Introductionmentioning

confidence: 99%

Spectral decomposition unlocks ascidian morphogenesis

Dokmegang,

Faure,

Lemaire

et al. 2023

Preprint

Self Cite

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Transformation of Pluripotency States during Morphogenesis of Mouse and Human Epiblast

Abdyev,

Alpeeva,

Kalistratova

et al. 2023

Russ J Dev Biol

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Transformation of Pluripotency States during Morphogenesis of Mouse and Human Epiblast

Abdyev,

Alpeeva,

Kalistratova

et al. 2023

Ontogenez

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The pluripotent status of a cell in vivo is spatio-temporally regulated within embryogenesis and is determined by the processes of self-renewal, endless proliferation and differentiation into all cell types of the body. Previously, the pluripotency was characterized using teratocarcinoma cells. Then this term was applied to the embryonic cells of the preimplantation mouse embryo. Preimplantationally formed mouse and human pluripotent stem cells (PSCs) appear to exist until gastrulation. One of the main events in the early mammalian development is the differentiation of the inner cell mass of the blastocyst (ICM) into a hypoblast and an epiblast, which develops into the embryo itself. Continuous and dynamic transformation of pluripotency states in development coincides with the morphogenetic processes, which are involved in the formation and maturation of the epiblast. Thus, blastocyst ICM cells differ in epigenetic and transcription patterns from their daughter cells forming the peri/post-implantation epiblast. With the onset of gastrulation movements, the maturation of epiblast cells ends with their differentiation into cells of three germ layers. This review considers the historical aspects of the study of cell pluripotency, various sources of PSCs, mechanisms and signaling pathways that support self-renewal and pluripotency in PSC cultures. In addition, we summarize and conceptualize data on morphogenetic processes that are involved in the formation of naive ICM cells in vivo and the subsequent maturation of mouse and human epiblast cells associated with the transformation of their pluripotency states.

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Modeling Epiblast Shape in Implanting Mammalian Embryos

Cited by 3 publications

References 58 publications

Spectral decomposition unlocks ascidian morphogenesis

Spectral decomposition unlocks ascidian morphogenesis

Transformation of Pluripotency States during Morphogenesis of Mouse and Human Epiblast

Transformation of Pluripotency States during Morphogenesis of Mouse and Human Epiblast

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