Modeling DNA condensation on freestanding cationic lipid membranes

Cherstvy, Andrey G.; Petrov, Eugene P.

doi:10.1039/c3cp53433b

Cited by 46 publications

(50 citation statements)

References 141 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has to be noted that a lot of work has been done on liposome-polyelectrolyte complexation by employing DNA, which is quite different in terms of flexibility with respect to NaPA, which has approximately the same charge density but a much higher flexibility, and a coexistence of different structures ranging from multilamellar DNA lipid complexes to unilamellar DNA-coated vesicles, as pd-liposome aggregates, has been observed [40,4]. Within this framework, similarly to what observed in the presence of DNA by several authors (see for example [13,41] and references therein), our results further confirm the ability to tune structure and morphology of the aggregates by means of lipid composition, ionic strength and polymer/lipid charge ratio. Moreover, due to the good biocompatibility of NaPA [42,43], the DOTAP/DOPC-NaPA complexes have also a specific interest for their potential use as multi-compartment vectors for multi-drug delivery [9].…”

Section: Introductionsupporting

confidence: 51%

Salt-induced reentrant stability of polyion-decorated particles with tunable surface charge density

Sennato

Carlini

Truzzolillo

et al. 2016

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

The electrostatic complexation between DOTAP/DOPC unilamellar liposomes and an oppositely charged polyelectrolyte (NaPA) has been investigated in a wide range of the liposome surface charge density. We systematically characterized the reentrant condensation and the charge inversion of polyelectrolyte-decorated liposomes by means of dynamic light scattering and electrophoresis. We explored the stability of this model polyelectrolyte/colloid system at different values of the surface charge of the bare liposomes and by changing two independent control parameters of the suspensions: the polyelectrolyte/colloid charge ratio and the ionic strength of the aqueous suspending medium. The progressive addition of neutral DOPC lipid within the liposome membrane gave rise to an interesting phenomenon which has not been observed previously: the stability diagram of the suspensions showed a novel reentrance due to the crossing of the desorption threshold of the polyelectrolyte. Indeed, at fixed charge density of the bare DOTAP/DOPC liposomes and for a wide range of polyion concentrations, we showed that the simple electrolyte addition first (low salt regime) destabilizes the suspensions because of the enhanced screening of the residual repulsion between the complexes, and then (high salt regime) determines the onset of a new stable phase, originated by the absence of polyelectrolyte adsorption on the particle surfaces. We show that the observed phenomenology can be rationalized within the modified Velegol-Thwar model for heterogeneously charged particles and that the polyelectrolyte desorption fits well the predictions of the adsorption theory of Winkler and Cherstvy [1]. Our findings unambiguously support the picture of the reentrant condensation as driven by the correlated adsorption of the polyelectrolyte chains on the particle surface, providing interesting insights into possible mechanisms for tailoring complex colloids via salt-induced effects.

show abstract

Section: Introductionsupporting

confidence: 51%

Salt-induced reentrant stability of polyion-decorated particles with tunable surface charge density

Sennato

Carlini

Truzzolillo

et al. 2016

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

show abstract

“…6 Some modern biosensors utilise lipid membranes to monitor binding of proteins. 7 Membrane-facilitated supramolecular aggregation of, for instance, proteins and nanoparticles, [8][9][10][11][12][13] larger particles such as proteins and virus-like colloidal particles 14,15 as well as membrane-driven condensation of linear DNA on freestanding lipid bilayer [16][17][18] is ubiquitous in biophysics. Assembly and pattern formation of colloidal particles of various shapes and surface properties on liquid interfaces is also an active field of research.…”

Section: Introductionmentioning

confidence: 99%

“…The deformations of the surrounding of a particle immersed in a liquid scales with the liquid contact angle due to wetting. This is reminiscent of deformations of cationic lipid membranes triggered by, for instance, electrostatic binding [16][17][18][35][36][37] of oppositely charged DNA molecules and fd filamentous virus particles. 38 [Ref.…”

Section: Introductionmentioning

confidence: 99%

Interactions of rod-like particles on responsive elastic sheets

et al. 2016

Self Cite

View full text Add to dashboard Cite

What are the physical laws of the mutual interactions of objects bound to cell membranes, such as various membrane proteins or elongated virus particles? To rationalise this, we here investigate by extensive computer simulations mutual interactions of rod-like particles adsorbed on the surface of responsive elastic two-dimensional sheets. Specifically, we quantify sheet deformations as a response to adhesion of such filamentous particles. We demonstrate that tip-to-tip contacts of rods are favoured for relatively soft sheets, while side-by-side contacts are preferred for stiffer elastic substrates. These attractive orientation-dependent substrate-mediated interactions between the rod-like particles on responsive sheets can drive their aggregation and self-assembly. The optimal orientation of the membrane-bound rods is established via responding to the elastic energy profiles created around the particles. We unveil the phase diagramme of attractive-repulsive rod-rod interactions in the plane of their separation and mutual orientation. Applications of our results to other systems featuring membrane-associated particles are also discussed.

show abstract

“…In this context it is worthwhile remarking that in the context of lipid membranes the topic of membranemediated attractive interactions between spherical colloidal particles [35][36][37] and cylindrical DNA molecules deposited on cationic lipid membranes [38,39] attracted significant theoretical and experimental attention in recent years. The reason for inter-particle attraction put forward in some fluctuation-based models is due to the tendency to reduce the inter-particle separation in order to diminish the area of a deformed membrane in which fluctuations are suppressed due to binding of adhesive particles.…”

Section: Dynamics: Network Deformations Around Two Viral Particlesmentioning

confidence: 99%

Deformation propagation in responsive polymer network films

Ghosh

Cherstvy

Metzler

2014

The Journal of Chemical Physics

Self Cite

View full text Add to dashboard Cite

We study the elastic deformations in a cross-linked polymer network film triggered by the binding of submicron particles with a sticky surface, mimicking the interactions of viral pathogens with thin films of stimulus-responsive polymeric materials such as hydrogels. From extensive Langevin Dynamics simulations we quantify how far the network deformations propagate depending on the elasticity parameters of the network and the adhesion strength of the particles. We examine the dynamics of the collective area shrinkage of the network and obtain some simple relations for the associated characteristic decay lengths. A detailed analysis elucidates how the elastic energy of the network is distributed between stretching and compression modes in response to the particle binding. We also examine the force-distance curves of the repulsion or attraction interactions for a pair of sticky particles in the polymer network film as a function of the particle-particle separation. The results of this computational study provide new insight into collective phenomena in soft polymer network films and may, in particular, be applied to applications for visual detection of pathogens such as viruses via a macroscopic response of thin films of cross-linked hydrogels.

show abstract

Modeling DNA condensation on freestanding cationic lipid membranes

Cited by 46 publications

References 141 publications

Salt-induced reentrant stability of polyion-decorated particles with tunable surface charge density

Salt-induced reentrant stability of polyion-decorated particles with tunable surface charge density

Interactions of rod-like particles on responsive elastic sheets

Deformation propagation in responsive polymer network films

Contact Info

Product

Resources

About