Modeling Chronic Dacryocystitis in Rabbits by Nasolacrimal Duct Obstruction with Self-Curing Resin

Abstract

We established a chronic dacryocystitis model by injecting of 0.05, 0.1, and 0.15 ml self-curing resin via the lacrimal punctum in rabbits. Animals were randomized into four groups (n = 11 animals/group). The control group received 0.15 ml normal saline. Within three months postinjection, epiphora and eye discharge were observed. At the 90th day postlacrimal passage irrigation, CT dacryocystography was performed to find changes in the lacrimal image, and hematoxylin and eosin staining was made to identify pathological changes of the lacrimal sac. Three months postinjection, the rabbits in control group and those who received 0.05 and 0.1 ml self-curing resin failed to develop chronic dacryocystitis. However, 8/11 (72.7%) rabbits those received 0.15 ml self-curing resin were symptomatic and showed complete reflux in lacrimal passage irrigation, indicating the obstruction of the nasolacrimal duct. CT dacryocystography showed that the obstruction was present only in the animals with chronic dacryocystitis. Pathological examinations of chronic dacryocystitis also revealed significantly inflammatory changes, such as mucus epithelium thickening, irregular papillary proliferation, and submucosal fibrous deposition. Local injection of 0.15 ml self-curing resin can induce permanent obstruction of the nasolacrimal duct in rabbits and establish a model of chronic dacryocystitis.

Cytocompatibility of 3D printed dental materials for temporary restorations on fibroblasts

“…4). This was thought to be caused by the difference in fabrication methods between DLP 3D printing and self-curing [32,33]. Therefore, research on biocompatibility regarding micro leakage of temporary restorative materials are recommended for future research.…”

Helical computed tomographic-dacryocystography in adult pet dwarf rabbits: procedure and normal appearance

“…In rabbits with an obstructive NLDS disease, CT-DCG might help to identify the site and the cause of obstruction, such as external compression (pathological backward overgrowth, bony lesion, extranasolacrymal mass), mural lesion (developmental, hyperplastic or fibrotic duct stenosis) and intraluminal lesion (intra-ductal pus or dacryolith). In a recent study, CT-DCG performed in rabbits with experimentally induced dacryocystitis exhibited abnormal findings, such as incomplete filling of the NLDS and dilation of the NLDS segment proximal to the site of stenosis (Hou et al, 2017).…”

Frequency of dental disease and associated pathologies in dwarf rabbits

“…Имеются данные, свидетельствующие о том, что ряд дентальных патологий может вызывать развитие эпифоры и вторичного дакроцистита [8,11]. Кроме этого, развитию дакриоцистита может способствовать инфекция носовых ходов [10]. Следовательно, у животных предрасположенных к дентальным патологиям и риносинуситу выше риск развития дакриоциститов.…”