Modeling Chronic Coccidioidomycosis in Mice

Shubitz, Lisa F.; Butkiewicz, Christine D.; Trinh, Hien T.

doi:10.1007/978-1-0716-3199-7_11

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…One of the challenges in studying disseminated infection in mice is the extreme virulence of Coccidioides infection in most inbred mouse strains. Intranasal infection of mice typically results in lethal pneumonia developing within 2-3 weeks; thus, there is insufficient time to develop the type of dissemination seen in human patients [106]. A recently described strain of C. posadasii displays growth delays in vitro and in vivo in mice, allowing for the infection to be assessed over a longer period.…”

Section: Animal Studiesmentioning

confidence: 99%

“…A recently described strain of C. posadasii displays growth delays in vitro and in vivo in mice, allowing for the infection to be assessed over a longer period. This strain has been proposed as a novel means for studying chronic infection [106,107]. Utilization of this C. posadasii strain, in combination with peripheral infection routes such as intraperitoneal or intraarticular injection, may provide new opportunities to study Coccidioides infection of the bones and/or joints in mice.…”

Section: Animal Studiesmentioning

confidence: 99%

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Coccidioidomycosis Osteoarticular Dissemination

Moni,

Wise,

Loots

et al. 2023

JoF

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Valley fever or coccidioidomycosis is a pulmonary infection caused by species of Coccidioides fungi that are endemic to California and Arizona. Skeletal coccidioidomycosis accounts for about half of disseminated infections, with the vertebral spine being the preferred site of dissemination. Most cases of skeletal coccidioidomycosis progress to bone destruction or spread to adjacent structures such as joints, tendons, and other soft tissues, causing significant pain and restricting mobility. Manifestations of such cases are usually nonspecific, making diagnosis very challenging, especially in non-endemic areas. The lack of basic knowledge and research data on the mechanisms defining susceptibility to extrapulmonary infection, especially when it involves bones and joints, prompted us to survey available clinical and animal data to establish specific research questions that remain to be investigated. In this review, we explore published literature reviews, case reports, and case series on the dissemination of coccidioidomycosis to bones and/or joints. We highlight key differential features with other conditions and opportunities for mechanistic and basic research studies that can help develop novel diagnostic, prognostic, and treatment strategies.

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Section: Animal Studiesmentioning

confidence: 99%

Section: Animal Studiesmentioning

confidence: 99%

Coccidioidomycosis Osteoarticular Dissemination

Moni,

Wise,

Loots

et al. 2023

JoF

View full text Add to dashboard Cite

show abstract

“…Одним из необходимых этапов проведения вышеперечисленных исследований являются эксперименты, связанные с моделированием кокцидиоидомикоза на биологической модели. Наиболее часто для этих целей используют лабораторных мышей как аутбредных, так и инбредных линий с различной восприимчивостью к возбудителям заболевания [6][7][8][9][10][11]. При заражении мышей вирулентными штаммами развивается преимущественно острая летальная форма кокцидиоидомикоза, хотя описано моделирование хронического течения заболевания или его возможной реактивации.…”

Section: Introductionunclassified

Estimation of the effectiveness of dexamethasone use in experimental modeling of coccidioidomycosis

Polovets,

Murugova,

Lipnitsky

et al. 2024

Immunopathol Allergol Infectol

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An integral part of fundamental research regarding the causative agents of coccidioidomycosis are experiments related to modeling the infectious process on a biological model. It has been shown that introducing an animal into a state of immunosuppression makes it possible to increase its susceptibility to pathogens and reproduce the generalization of the process in a shorter time. To model immunosuppression in experimental animals, the use of a synthetic glucocorticosteroid, dexamethasone, has become widespread. The purpose of our work was to study the effectiveness of the use of dexamethasone in modeling immunosuppression in animals with experimental coccidioidomycosis. Materials and methods. The infectious process was modeled by a single intraperitoneal injection of a fungal suspension into BALB/c mice and outbred mice with artificial immunosuppression caused by dexamethasone. Pathoanatomical dissection of dead and sacrificed mice was performed on the same day. Species identification of the isolated cultures was carried out using light, fluorescence and electron microscopy. Results. Infection of mice with strains of coccidioidomycosis pathogens was accompanied by the development of clinical symptoms, and in some cases, the death of animals. Characteristic pathological-anatomical changes were observed in the fallen individuals. Quadruple intraperitoneal administration of dexamethasone led to significant differences in the life expectancy of animals when compared with groups of mice without immunosuppression (p<0.05). Conclusions. This study demonstrated the effectiveness of dexamethasone in modeling acute coccidioidomycosis. It has been shown that intraperitoneal administration of dexamethasone at a concentration of 0.04 mg/mouse for 4 days before infection leads to increased susceptibility of the animal and, as a consequence, the development of an acute form of the disease.

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From soil to clinic: current advances in understanding Coccidioides and coccidioidomycosis

Jackson,

Teixeira,

Barker

2024

Microbiol Mol Biol Rev

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SUMMARY Coccidioides immitis and Coccidioides posadasii are fungal pathogens that cause systemic mycoses and are prevalent in arid regions in the Americas. While C. immitis mainly occurs in California and Washington, C. posadasii is widely distributed across North and South America. Both species induce coccidioidomycosis (San Joaquin Valley fever or, more commonly, Valley fever), with reported cases surging in the United States, notably in California and Arizona. Moreover, cases in Argentina, Brazil, and Mexico are on the rise. Climate change and environmental alterations conducive to Coccidioides spp. proliferation have been recently explored. Diagnostic challenges contribute to delayed treatment initiation, compounded by limited therapeutic options. Although antifungal drugs are often effective treatments, some patients do not respond to current therapies, underscoring the urgent need for a vaccine, particularly for vulnerable populations over 60 years old relocating to endemic areas. Despite recent progress, gaps persist in the understanding of Coccidioides ecology, host immune responses, and vaccine development. This review synthesizes recent research advancements in Coccidioides ecology, genomics, and immune responses, emphasizing ongoing efforts to develop a human vaccine.

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Modeling Chronic Coccidioidomycosis in Mice

Cited by 3 publications

References 19 publications

Coccidioidomycosis Osteoarticular Dissemination

Coccidioidomycosis Osteoarticular Dissemination

Estimation of the effectiveness of dexamethasone use in experimental modeling of coccidioidomycosis

From soil to clinic: current advances in understanding Coccidioides and coccidioidomycosis

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