Modeling ANXA2-overexpressing circulating tumor cells homing and high throughput screening for metastasis impairment in endometrial carcinomas

Herrero, Carolina; Brea, José; Pérez-Díaz, Amparo; Cuadrado, Emiliano; Ferreño, Noelia; Moiola, Cristian P.; Colás, Eva; López‐López, Rafael; Loza, Marı́a Isabel; Abal, Miguel; Alonso-Alconada, Lorena

doi:10.1016/j.biopha.2021.111744

Cited by 2 publications

(2 citation statements)

References 45 publications

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“…This idea is supported by a certain similarity between endometriosis and metastatic cancer, especially since the elevated level of ANXA2 protein is already known to be typically present in many cancers, including endometrial cancer. [48][49][50][51][52] These authors also consider monocytes to be cells that secrete ANXA2 in exosomes. In the same study it was also stated that endometriosis in stages I and II may differ in its pathogenesis from that in stages III and IV, resulting in a difference in ANXA2 concentrations, and this may create the use of ANXA2 as a biomarker differentiating endometriosis stages.…”

Section: Discussionmentioning

confidence: 99%

Plasma and Peritoneal Fluid Annexin A2 Levels in Patients with Endometriosis

Zygarowicz,

Kacperczyk-Bartnik,

Sierdzinski

et al. 2023

JIR

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Introduction Endometriosis is an inflammatory-related reproductive age disease characterized by the presence of endometrial cells outside the uterine cavity. Current laboratory practice does not provide specific markers for detecting and assessing the advancement of endometriosis in either plasma or peritoneal fluid. The severity of disease is assessed in stages from I to IV based on the results of laparoscopic inspection. The protein annexin A2 (ANXA2) has been reported to be associated with inflammatory processes. Aim of the Study The study aimed to investigate and compare ANXA2 protein concentration using the ELISA method in plasma and peritoneal fluid in a group of women with endometriosis compared to controls. Materials and Methods Biological material was collected during a multicenter, cross-sectional study, which was conducted at eight departments during elective laparoscopy from 53 women with and 40 women without endometriosis. Patients were divided by endometriosis stage and infertility status, and then compared with subgroups. Analysis included the Chi-square test for categorical variables, Mann–Whitney U -test and two-sided Wilcoxon rank-sum test for continuous variables. Results Women with endometriosis had significantly elevated plasma ANXA2 levels compared to women without endometriosis (mean concentrations 28.69 vs 19.61 ng/L, p=0.01). Differences in peritoneal fluid ANXA2 levels were statistically insignificant (mean concentrations of 23.7 vs 22.97 ng/L, p=0.06). Plasma concentrations in patients with stage III and IV endometriosis were significantly higher compared to controls (mean concentrations of 24.19 vs 19.71 ng/L, p=0.03). No such differences were observed in plasma when comparing stages I–II vs III–IV, and stages I–II vs controls (mean concentrations of 33.82 vs 24.19 ng/L, p=0.72 and 33.82 vs 19.71 ng/L, p=0.12, respectively). Comparison of samples from patients with or without infertility, primary or secondary infertility, endometriosis with or without infertility, and non-endometriosis with or without infertility showed no significant differences in the plasma nor in the peritoneal fluid concentrations. Conclusion ANXA2 is possibly involved in the pathogenesis of endometriosis, especially in advanced stages. Due to the limited group of tested samples, further studies are needed to confirm its role.

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Section: Discussionmentioning

confidence: 99%

Plasma and Peritoneal Fluid Annexin A2 Levels in Patients with Endometriosis

Zygarowicz,

Kacperczyk-Bartnik,

Sierdzinski

et al. 2023

JIR

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“…The authors of [ 6 ] validated the ANXA2 expression in CTC from great risk of Endometrial Cancer sufferers. The cancer cell adherence of ANXA2 indicating CTC to the endothelium and localization for the development of micro-metastasis were then simulated in vitro and in vivo.…”

Section: Related Workmentioning

confidence: 99%

Research on the Guiding Effect of CTCs on Postoperative Adjuvant Therapy for Patients with Early Stage Endometrial Cancer

Zhai

Zhang

et al. 2022

Journal of Oncology

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Endometrial tumor has increased in occurrence and fatality in China during the last 11 years, owing to inconsistent hormone use and modifications in people living surrounding and lifestyles. One of the three main gynaecological tumors is endometrial carcinoma (EC). Longer waiting duration of operation was linked to a lower chance of sustainability in endometrial tumor patients. Despite the great sustainability rate of endometrial tumor, only around 46 percent of patients undergo adjuvant treatment. Circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), and circulating free DNA (cfDNA) are the most investigated tumor noninvasive indicators. These circulating biomarkers are important in the knowledge of metastasis and tumorigenesis, and they could help researchers comprehend how cancer dynamics evolve throughout the therapy and illness development. In patients with solid tumor, the existence of circulating tumor cells (CTCs) in the peripheral blood is linked to a weak prognosis. However, there is a scarcity of information on how to detect CTCs in endometrial cancer (EC). Hence, in this paper, we analyze the guiding effect of CTCs on postoperative adjuvant treatment for sufferers with initial phase endometrial tumor using multi-cox regression method. The dataset is selected and the blood samples are collected using plasma separation method. The CTC is detected using differential diagnosis. The morphology and biological features, Immunocytochemistry, Genomic analysis, Transcriptomic analysis, Proteomic analysis, and molecular analysis are performed and the outcomes are evaluated.

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Modeling ANXA2-overexpressing circulating tumor cells homing and high throughput screening for metastasis impairment in endometrial carcinomas

Cited by 2 publications

References 45 publications

Plasma and Peritoneal Fluid Annexin A2 Levels in Patients with Endometriosis

Plasma and Peritoneal Fluid Annexin A2 Levels in Patients with Endometriosis

Research on the Guiding Effect of CTCs on Postoperative Adjuvant Therapy for Patients with Early Stage Endometrial Cancer

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