Modeling angiogenesis in the human brain in a tissue-engineered post-capillary venule

Zhao, Nan; Kulkarni, Sarah; Zhang, Sophia; Linville, Raleigh M.; Chung, Tracy D.; Guo, Zhaobin; Jamieson, John J.; Norman, Danielle; Liang, Lily R.; Pessell, Alexander F; Searson, Peter C.

doi:10.1007/s10456-023-09868-7

Cited by 10 publications

(10 citation statements)

References 78 publications

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“…One limitation of these iBMECL structures is that they cannot be perfused, hindering the examination of functions, such as barrier transport. While models incorporating fluid perfusion may be better suited to investigating questions of this nature (Kurosawa et al, 2022;Zhao et al, 2023) bioprinted cultures supports previous literature using other natural and synthetic-based matrices (Brannvall et al, 2007;Pellett et al, 2015;Ranjan et al, 2020). The enhancement of neuronal function in 3D culture is in line with previous reports of primary rodent neurons within a number of 3D matrices and found the signaling patterns to be more reflective of in vivo neuronal activity (Bosi et al, 2015;Bourke et al, 2018).…”

Section: Bioprinted Npcs Remain Viable and Enhance Neuronal Different...supporting

confidence: 84%

“…One limitation of these iBMECL structures is that they cannot be perfused, hindering the examination of functions, such as barrier transport. While models incorporating fluid perfusion may be better suited to investigating questions of this nature (Kurosawa et al, 2022 ; Zhao et al, 2023 ), our model offers increased flexibility of gel composition, automaticity, and compatibility with standard plate formats. This underscores the importance of developing a suite of novel 3D techniques to improve our understanding of in vitro vascular biology.…”

Section: Discussionmentioning

confidence: 99%

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Three‐dimensional bioprinting of stem cell‐derived central nervous system cells enables astrocyte growth, vasculogenesis, and enhances neural differentiation/function

Sullivan

Lane

Volkerling³

et al. 2023

Biotech & Bioengineering

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Current research tools for preclinical drug development such as rodent models and two‐dimensional immortalized monocultures have failed to serve as effective translational models for human central nervous system (CNS) disorders. Recent advancements in the development of induced pluripotent stem cells (iPSCs) and three‐dimensional (3D) culturing can improve the in vivo‐relevance of preclinical models, while generating 3D cultures though novel bioprinting technologies can offer increased scalability and replicability. As such, there is a need to develop platforms that combine iPSC‐derived cells with 3D bioprinting to produce scalable, tunable, and biomimetic cultures for preclinical drug discovery applications. We report a biocompatible poly(ethylene glycol)‐based matrix which incorporates Arg‐Gly‐Asp and Tyr‐Ile‐Gly‐Ser‐Arg peptide motifs and full‐length collagen IV at a stiffness similar to the human brain (1.5 kPa). Using a high‐throughput commercial bioprinter we report the viable culture and morphological development of monocultured iPSC‐derived astrocytes, brain microvascular endothelial‐like cells, neural progenitors, and neurons in our novel matrix. We also show that this system supports endothelial‐like vasculogenesis and enhances neural differentiation and spontaneous activity. This platform forms a foundation for more complex, multicellular models to facilitate high‐throughput translational drug discovery for CNS disorders.

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Section: Bioprinted Npcs Remain Viable and Enhance Neuronal Different...supporting

confidence: 84%

“…One limitation of these iBMECL structures is that they cannot be perfused, hindering the examination of functions, such as barrier transport. While models incorporating fluid perfusion may be better suited to investigating questions of this nature (Kurosawa et al, 2022 ; Zhao et al, 2023 ), our model offers increased flexibility of gel composition, automaticity, and compatibility with standard plate formats. This underscores the importance of developing a suite of novel 3D techniques to improve our understanding of in vitro vascular biology.…”

Section: Discussionmentioning

confidence: 99%

Three‐dimensional bioprinting of stem cell‐derived central nervous system cells enables astrocyte growth, vasculogenesis, and enhances neural differentiation/function

Sullivan

Lane

Volkerling³

et al. 2023

Biotech & Bioengineering

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“…The “turbulent flow” and “increased fluid shear stress” in the blood vessel are the main initiating factors for activation of vascular endothelial cells, which can promote vascular sprouting and collateral angiogenesis. Therefore, from a clinical and practical point of view, distal arteriovenous bundle ligation is an effective method of vascularization ( Galie et al, 2014 ; Zhang et al, 2022 ; Zhao et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Construction of a vascularized fascia-prosthesis compound model with axial pedicle for ear reconstruction surgery

Chen

Shan

et al. 2023

Front. Bioeng. Biotechnol.

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Background: To design a vascular pedicled fascia-prosthesis compound model that can be used for ear reconstruction surgery.Methods: A vascularized tissue engineering chamber model was constructed in New Zealand rabbits, and fresh tissues were obtained after 4 weeks. The histomorphology and vascularization of the newly born tissue compound were analyzed and evaluated by tissue staining and Micro-CT scanning.Results: The neoplastic fibrous tissue formed in the vascularized tissue engineering chamber with the introduction of abdominal superficial vessels, similar to normal fascia, was superior to the control group in terms of vascularization, vascular density, total vascular volume, and total vascular volume/total tissue volume.Conclusion:In vivo, introducing abdominal superficial vessels in the tissue engineering chamber prepped for ear prosthesis may form a well-vascularized pedicled fascia-prosthesis compound that can be used for ear reconstruction.

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“…For instance, in organs like the kidney and liver, the endothelium is discontinuous, permitting fluid and molecular exchange [ 120 ]. In the cerebral vasculature, the blood–brain barrier is made up of tightly packed ECs that are largely surrounded by pericytes, restricting paracellular transportation of specific transport proteins [ 121 ]. The characteristics of the various cell types are summarized in Table 3 .…”

Section: Cell Source For Blood Capillary Formationmentioning

confidence: 99%

“…However, these cells lack the crucial features of brain microvascular endothelial cells (BMECs). High trans-endothelial electrical resistance limits paracellular permeability and exocytosis activity, making these models unsuitable for studying the characteristic features of vascular networks [ 121 , 130 , 131 ].…”

Section: Cell Source For Blood Capillary Formationmentioning

confidence: 99%

Technology for the formation of engineered microvascular network models and their biomedical applications

Li,

Shang,

Zeng

et al. 2024

Nano Convergence

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Tissue engineering and regenerative medicine have made great progress in recent decades, as the fields of bioengineering, materials science, and stem cell biology have converged, allowing tissue engineers to replicate the structure and function of various levels of the vascular tree. Nonetheless, the lack of a fully functional vascular system to efficiently supply oxygen and nutrients has hindered the clinical application of bioengineered tissues for transplantation. To investigate vascular biology, drug transport, disease progression, and vascularization of engineered tissues for regenerative medicine, we have analyzed different approaches for designing microvascular networks to create models. This review discusses recent advances in the field of microvascular tissue engineering, explores potential future challenges, and offers methodological recommendations.

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Modeling angiogenesis in the human brain in a tissue-engineered post-capillary venule

Cited by 10 publications

References 78 publications

Three‐dimensional bioprinting of stem cell‐derived central nervous system cells enables astrocyte growth, vasculogenesis, and enhances neural differentiation/function

Three‐dimensional bioprinting of stem cell‐derived central nervous system cells enables astrocyte growth, vasculogenesis, and enhances neural differentiation/function

Construction of a vascularized fascia-prosthesis compound model with axial pedicle for ear reconstruction surgery

Technology for the formation of engineered microvascular network models and their biomedical applications

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