“…In EH the algae cell wall carbohydrates are biochemically broken down into sugar monomers . In effect, this makes the algae’s lipids and amino acids accessible in the hydrolysate. , Since most microalgae biomass production is constrained to high quality-nutrients and chemical products harsh techniques such as acid and alkaline thermal pre-treatment ,, must be avoided to acquire algae lipid oils, carbohydrates, and amino acids safely to get the most effective value out of the biomass’s cell content. ,, Although EH can be technically cumbersome and can require higher material cost expenditure compared to other processes at large scales, , it was chosen as it is an efficient and sustainable means of using enzymes to convert the cell’s wall into intermediates to derive valuable products while avoiding the toxicity, inhibitors, and waste streams of chemical hydrolysis and the high costs and energy intensity of thermal hydrolysis − or mechanical pre-treatment such as high-pressure homogenization (HPH) or bead or ball milling. ,− After EH, the algae biomass slurry is dried at low temperature in a mechanical vapor re-compressor to roughly 40.0% solids to ease accessibility to algae hydrolysate’s contents . The processing conditions and resources for EH are derived from the experimental procedures of the Technical University of Munich’s (TUM) Werner Siemens Chair of Synthetic Biotechnology (WSSB) for various algae species…”