Model-supported optimization of phototrophic growth in a stirred-tank photobioreactor

Franco‐Lara, Ezequiel; Havel, Jan; Peterat, Frank; Weuster‐Botz, Dirk

doi:10.1002/bit.21086

Cited by 33 publications

(18 citation statements)

References 39 publications

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“…Reported half-maximum rate constants for LI (K LI ) range between 2 and 21 W/m 2 (Cornet et al, 1995;De Nobel et al, 1998;Franco-Lara et al, 2006), which are near the average LI values in the PBR for Run II-2 and supports that the high concentration of cells led to a relatively low specific growth rate especially for 7.5% CO 2 due to significant LI limitation. Thus, LI should become the limiting factor above a critical biomass concentration that depends on PBR configuration and phototrophic strain.…”

Section: Light Attenuationmentioning

confidence: 79%

“…Based on the configuration of our PBR, the light availability can be explained with light-attenuation that is well represented by the Beer-Lambert's law (Franco-Lara et al, 2006;Suh and Lee, 2003). As shown in Equation ( 3), available LI decreases exponentially The temperature varied from 26 to 458C over the day-night cycle for Phase I, but was held constant at 308C for Phase II.…”

Section: Light Attenuation In Pbrmentioning

confidence: 99%

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Photoautotrophic nutrient utilization and limitation during semi‐continuous growth of Synechocystis sp. PCC6803

Kim

Vannela

Zhou

et al. 2010

Biotech & Bioengineering

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Microbial photosynthesis presents a valuable opportunity to capture abundant light energy to produce renewable bioenergy and biomaterials. To understand the factors that control the productivity of photosynthetic microorganisms, we conducted a series of semi-continuous experiments using bench-scale photobioreactor (PBR) systems, the cyanobacterium Synechocystis PCC6803 (PCC6803), and light conditions imitating actual day-night light irradiance (LI). Our results demonstrate that using normal BG-11 medium resulted in severe phosphate (P(i)) limitation for continuous operation. Mitigation of P(i)-limitation, by augmenting the P(i) content of BG-11, allowed higher biomass productivity; however, once P(i)-limitation was alleviated, limitation by inorganic carbon (C(i)) or LI occurred. C(i)-limitation was detected by a low total C(i) concentration (<5 mg C/L) and high and fluctuating pH. C(i)-limitation was relieved by delivering more CO(2), which led to a stable pH in the range of 7-9 and at least 5 mg/L of C(i) in HCO(3)(-). LI limitation, evidenced by an average LI <14 W/m(2) for PCC6803, was induced by a high biomass concentration of 1,300 mg/L. Thus, this work provides quantitative tools of stoichiometry and kinetics to evaluate limitation on PBRs.

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Section: Light Attenuationmentioning

confidence: 79%

Section: Light Attenuation In Pbrmentioning

confidence: 99%

Photoautotrophic nutrient utilization and limitation during semi‐continuous growth of Synechocystis sp. PCC6803

Kim

Vannela

Zhou

et al. 2010

Biotech & Bioengineering

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“…strain PCC 7942. Synechococcus cells were cultivated on a 20-liter scale in BG-11 medium (1) according to the method of Franco-Lara et al (6). For cell disruption, 95 mg ml Ϫ1 of Synechococcus sp.…”

Section: Chemicals and Enzymesmentioning

confidence: 99%

Identification, Cloning, and Characterization of a Novel Ketoreductase from the Cyanobacterium Synechococcus sp. Strain PCC 7942

Hölsch

Havel

Haslbeck

et al. 2008

Appl Environ Microbiol

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A new ketoreductase useful for asymmetric synthesis of chiral alcohols was identified in the cyanobacterium Synechococcus sp. strain PCC 7942. Mass spectrometry of trypsin-digested peptides identified the protein as 3-ketoacyl-[acyl-carrier-protein] reductase (KR) (EC 1.1.1.100). The gene, referred to as fabG, was cloned, functionally expressed in Escherichia coli, and subsequently purified to homogeneity. The enzyme displayed a temperature optimum at 44°C and a broad pH optimum between pH 7 and pH 9. The NADPH-dependent KR was able to asymmetrically reduce a variety of prochiral ketones with good to excellent enantioselectivities (>99.8%). The KR showed particular high specific activity for asymmetric reduction of ethyl 4-chloroacetoacetate (38.29 ؎ 2.15 U mg

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“…This leads to a lower incidence of dark zones inside the reactor and higher biomass productivity. However, the main disadvantage of this system is low surface area to volume ratio, which in turn decreases light harvesting efficiency (Franco-Lara et al 2006). Moreover, mechanical agitation generates much more heat than the sparging of compressed gas; hence, stirred PBRs are expensive to operate and maintain.…”

Section: Stirred Tank Pbrsmentioning

confidence: 99%

A mini review: photobioreactors for large scale algal cultivation

Gupta

Lee

Choi

2015

World J Microbiol Biotechnol

236

103

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Microalgae cultivation has gained much interest in terms of the production of foods, biofuels, and bioactive compounds and offers a great potential option for cleaning the environment through CO2 sequestration and wastewater treatment. Although open pond cultivation is most affordable option, there tends to be insufficient control on growth conditions and the risk of contamination. In contrast, while providing minimal risk of contamination, closed photobioreactors offer better control on culture conditions, such as: CO2 supply, water supply, optimal temperatures, efficient exposure to light, culture density, pH levels, and mixing rates. For a large scale production of biomass, efficient photobioreactors are required. This review paper describes general design considerations pertaining to photobioreactor systems, in order to cultivate microalgae for biomass production. It also discusses the current challenges in designing of photobioreactors for the production of low-cost biomass.

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Model-supported optimization of phototrophic growth in a stirred-tank photobioreactor

Cited by 33 publications

References 39 publications

Photoautotrophic nutrient utilization and limitation during semi‐continuous growth of Synechocystis sp. PCC6803

Photoautotrophic nutrient utilization and limitation during semi‐continuous growth of Synechocystis sp. PCC6803

Identification, Cloning, and Characterization of a Novel Ketoreductase from the Cyanobacterium Synechococcus sp. Strain PCC 7942

A mini review: photobioreactors for large scale algal cultivation

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