Model simulations for genetic random drift in the outbred strain Fzt:DU

Dietl, G.; Langhammer, Martina; Renne, Ulla

doi:10.5194/aab-47-595-2004

Cited by 33 publications

(46 citation statements)

References 4 publications

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“…In this study, on one hand, a non-inbred mouse line that has been generated by selection over 90 generations for high treadmill performance (DUhTP) (Falkenberg et al 2000) and on the other control mice (DUC) that had been generated from the identical base population without phenotype selection was used (Dietl et al 2004). The males were housed under controlled environmental conditions in a semi-barrier system with a 12-h light–12-h dark cycle (room temperature = 22.5 ± 0.2 °C, humidity = 50–60 %) as described in (Brenmoehl et al 2013).…”

Section: Methodsmentioning

confidence: 99%

Browning of subcutaneous fat and higher surface temperature in response to phenotype selection for advanced endurance exercise performance in male DUhTP mice

et al. 2016

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For the assessment of genetic or conditional factors of fat cell browning, novel and polygenic animal models are required. Therefore, the long-term selected polygenic mouse line DUhTP originally established in Dummerstorf for high treadmill performance is used. DUhTP mice are characterized by increased fat accumulation in the sedentary condition and elevated fat mobilization during mild voluntary physical activity. In the present study, the phenotype of fat cell browning of subcutaneous fat and a potential effect on oral glucose tolerance, an indicator of metabolic health, were addressed in DUhTP mice. Analysis of peripheral fat pads revealed increased brite (brown-in-white) subcutaneous adipose tissues and in subcutaneous fat from DUhTP mice higher levels of irisin and different markers of fat cell browning like T-box transcription factor (Tbx1), PPARα, and uncoupling protein (UCP1) (P < 0.05) when compared to unselected controls. UCP1 was further increased in subcutaneous fat from DUhTP mice in response to mild exercise (fourfold, P < 0.05). In addition, surface temperature of DUhTP mice was increased when compared to controls indicating a physiological effect of increased UCP1 expression. The present study suggests that DUhTP mice exhibit different markers of mitochondrial biogenesis and fat browning without external stimuli. At an age of 43 days, sedentary DUhTP mice have improved metabolic health as judged from lower levels of blood glucose after an oral glucose tolerance test. Consequently, the non-inbred mouse model DUhTP represents a novel model for the identification of fat cell browning mechanisms in white adipose tissues.

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Section: Methodsmentioning

confidence: 99%

Browning of subcutaneous fat and higher surface temperature in response to phenotype selection for advanced endurance exercise performance in male DUhTP mice

et al. 2016

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“…We used a non inbred mouse line (DU6P) established by phenotype selection over 138 generations for high protein mass at an age of 42 d after birth [27], [28]. In addition, an unselected control mouse line Fzt:DU (generation 142) was used.…”

Section: Methodsmentioning

confidence: 99%

Phenotype Selection Reveals Coevolution of Muscle Glycogen and Protein and PTEN as a Gate Keeper for the Accretion of Muscle Mass in Adult Female Mice

et al. 2012

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We have investigated molecular mechanisms for muscle mass accretion in a non-inbred mouse model (DU6P mice) characterized by extreme muscle mass. This extreme muscle mass was developed during 138 generations of phenotype selection for high protein content. Due to the repeated trait selection a complex setting of different mechanisms was expected to be enriched during the selection experiment. In muscle from 29-week female DU6P mice we have identified robust increases of protein kinase B activation (AKT, Ser-473, up to 2-fold) if compared to 11- and 54-week DU6P mice or controls. While a number of accepted effectors of AKT activation, including IGF-I, IGF-II, insulin/IGF-receptor, myostatin or integrin-linked kinase (ILK), were not correlated with this increase, phosphatase and tensin homologue deleted on chromosome 10 (PTEN) was down-regulated in 29-week female DU6P mice. In addition, higher levels of PTEN phosphorylation were found identifying a second mechanism of PTEN inhibition. Inhibition of PTEN and activation of AKT correlated with specific activation of p70S6 kinase and ribosomal protein S6, reduced phosphorylation of eukaryotic initiation factor 2α (eIF2α) and higher rates of protein synthesis in 29-week female DU6P mice. On the other hand, AKT activation also translated into specific inactivation of glycogen synthase kinase 3ß (GSK3ß) and an increase of muscular glycogen. In muscles from 29-week female DU6P mice a significant increase of protein/DNA was identified, which was not due to a reduction of protein breakdown or to specific increases of translation initiation. Instead our data support the conclusion that a higher rate of protein translation is contributing to the higher muscle mass in mid-aged female DU6P mice. Our results further reveal coevolution of high protein and high glycogen content during the selection experiment and identify PTEN as gate keeper for muscle mass in mid-aged female DU6P mice.

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“…All three mouse lines were originally derived from an outbred line, which was generated by systematic cross-breeding of four inbred (CBA/Bln, AB/Bln, C57BL/Bln, XVII/Bln) and four outbred (NMRI orig., Han:NMRI, CFW, CF1) lines [15,16]. From this genetic pool, the two independent high-fecundity lines, FL1 and FL2, were selected for an index trait, combining litter size (LSO) and litter weight (LWO) at birth in primiparous females (Index=1.6 × LSO + LWO) [17] up to generation 126.…”

Section: Selective Breeding For High and Normal Fecunditymentioning

confidence: 99%

Occurrence of Polyovular Follicles in Mouse Lines Selected for High Fecundity

Alm

Kuhlmann

Langhammer

et al. 2010

Journal of Reproduction and Development

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Abstract. The aim of this study was to evaluate the prevalence of follicles with more than one oocyte (polyovular follicles, POFs) in mouse lines selected for high fecundity. The ovaries of 18 mice, 6 each from 3 different lines, were examined to evaluate the number of POFs and the follicular histology. Polyovular follicles were observed in the two high fecundity breeds, FL1 and FL2, as well as in the unselected control line, DUKsi. The highest number of POFs per ovary (27.0 ± 7.2) was found in the FL1 line. The FL2 and DUKsi lines had 1.9 ± 0.7 and 0.6 ± 0.3 polyovular follicles per ovary, respectively. Most of the POFs contained 2 oocytes (>80%), but occasionally follicles containing up to 7 oocytes were observed. Follicles with more than 2 oocytes were observed in the FL1 line only. Key words: High fecundity, Mice, Polyovular follicles (POFs) (J. Reprod. Dev. 56: [449][450][451][452][453] 2010) n normal adult mouse ovaries, follicles contain a germ cell surrounded by one or more layers of granulosa cells, according to the follicle developmental stage. However, follicles with more than one oocyte are rarely found [1]. During initial development, multiple germ cells are present within the individualizing follicles, although in most cases, excess cells are eliminated and mammalian ovarian follicles contain only one germ cell per follicle. Follicles with more than one oocyte are referred to as multi oocyte follicles (MOFs) or polyovular follicles (POFs). They have been reported to occur in the ovaries of humans [2], primates [3], dogs [4,5], cats [6], goats [7] and mice [8]. In rats and mice, the incidence of POFs increases when the animals are exposed to estrogen analogs such as diethylstilbestrol, estradiol benzoate or genistein in a dose-dependent manner [9,10]. The POFs observed in mice and humans have been postulated to be remnants of oocyte clusters that did not separate, and which have become enclosed in follicles during neonatal primordial follicle assembly [2,10,11].Fecundity (the number of offspring per litter) can be related to an increase in the number of ova ovulated or in the efficiency of fertilization and embryo development. Booroola sheep have multiple offspring because of an increase in the number of follicles ovulated, which is related to the fecundity gene FecB, a mutation in bone morphogenetic protein receptor IB. The presence of FecB increases litter size by one or two extra lambs [12]. Polyovular follicles have been especially noted in bitches [13]. The presence of POFs could affect the number of oocytes recovered in assisted reproductive protocols and may also influence the ovulation rate and prolificity of these animals [5].Previous analysis of fecundity traits revealed that in high fecundity mouse lines (FL1, FL2), the number of ova ovulated is higher than the number of corpora lutea (CL) present. This is particularly evident in the FL1 line [14]. This observation suggests that individual follicles release more than one oocyte. The occurrence of polyovular follicles in specific strain...

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Model simulations for genetic random drift in the outbred strain Fzt:DU

Cited by 33 publications

References 4 publications

Browning of subcutaneous fat and higher surface temperature in response to phenotype selection for advanced endurance exercise performance in male DUhTP mice

Browning of subcutaneous fat and higher surface temperature in response to phenotype selection for advanced endurance exercise performance in male DUhTP mice

Phenotype Selection Reveals Coevolution of Muscle Glycogen and Protein and PTEN as a Gate Keeper for the Accretion of Muscle Mass in Adult Female Mice

Occurrence of Polyovular Follicles in Mouse Lines Selected for High Fecundity

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