Model, Simulation, and Experiments on Moving Exchange Boundary via Ligand and Quantum Dots in Chip Electrophoresis

Abstract

Herein, the quench model of the moving exchange boundary (MEB) was first created via a ligand of 5,5′-dithiobis(2nitro-benzoic acid) (DTNB) and group of 3-mercaptopropionic acid (MPA) capped on QDs, and then the recovery model was formed via MPA and 2-nitro-5-thiobenzoic acid (TNB) capped on QDs. The theory on MEB dynamics and width was developed based on the two reversible models, the simulation was conducted for the illumination of MEB, and the protocol was described for the MEB runs. The experiments revealed that (i) the quench model could be created via DTNB and MPA capped on QDs and the recovery one could be in situ formed via MPA and TNB capped on QDs, showing the feasibility of MEB models; (ii) the simulations on MEB dynamics and width were in coincidence with the theoretic predictions, showing the validity of two models; and (iii) the experiments demonstrated the validity of models, predictions, and simulations. The models and theory have potential for development of a biosensor, nanoparticle characterization, separation science, and an affinity assay of ligand-QDs.

A facile double moving redox boundary model for visual electrophoresis titration of ascorbic acid

“…Since 1970, a series of systematic investigations have been performed on the six moving reaction boundaries (MRB), for example, moving neutralization boundary (MNB) [24][25][26][27][28][29], precipitate boundary [24,30,31], chelation boundary [32], and affinity or recognition boundary [33,34] as well as exchange boundary [35]. Especially, the concept of MRB has shown its valuable applications in separation, quantitative analysis, and sample stacking [25].…”

Simulation and Verification of the Direct Current Electric Field on Fabricating High Porosity f-MWCNTs Thin Films by Electrophoretic Deposition Technique

“…Simulation in the EPD process allows us to better understand the electromigration process and determine the optimal deposition conditions without conducting experiments. Many works have explored the simulation of EPD process thus far. − These studies have contributed to the understanding of the processes of zone electrophoresis, − moving boundary electrophoresis, , isotachophoresis, − isoelectric focusing, − and isoelectric trapping . However, no specific studies have clearly shown the effect of the electric field on the thin film structure formed through cathodic electrophoretic deposition of f-MWCNTs.…”