As a promising pollutant emission reduction technology, biomass mixed combustion has attracted widespread attention worldwide. This paper aimed to study the characteristics of biomass mixed combustion and temperature distribution. A combination of simulation and experimental methods was adopted. The results showed when four kinds of biomass were burned separately, their highest temperatures in the center section of combustion chamber were corn stalk>cotton stalk>sawdust>rice straw in descending order. Compared with other three biomass, the highest temperature of corn stalk was more than 100 K higher, which mainly occurred during the full combustion stage, mainly because corn stalk had high volatile content and caught fire easily. In addition, with the optimal mixed combustion parameters, biomass mixed combustion improved the combustion characteristics of single biomass combustion. The optimal blending ratio of corn stalk to rice straw was 7:3, and the optimal primary air velocity and temperature were 48 m/s and 1300 K, respectively. With the optimal blending ratio, the maximum temperature in the center section was higher than that of single biomass combustion, with advanced ignition point, relatively uniform temperature distribution in the combustion chamber and good combustion performance, because the precipitation and combustion of high volatile components during mixed combustion caused the surface temperature of fixed carbon to rise rapidly to reach the ignition temperature. Finally, this paper studied the combustion characteristics of corn stalk and rice straw with the optimal mixed combustion parameters in mixed combustion experiment, and verified the good consistency between the simulation and experimental values. Therefore, biomass mixed combustion technology provides an important reference for solving the problem of low calorific value of single biomass combustion.