Model of Vibrio cholerae biofilm as a mechanism of its survival in surface water reservoirs

Куликалова, Е. С.; Саппо, С Г; Urbanovich, L. Ya.; Markov, E. Yu.; Mironova, L. V.; Балахонов, С. В.

doi:10.1134/s1995425514010089

Cited by 2 publications

(2 citation statements)

References 20 publications

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“…As expected, knockdown of rpoS in E. cloacae also resulted in decreased expression of the majority of RpoS-dependent stress resistance related genes ( bfr , dps , funC , katE 、 uspB , uspC , uspE , uspF ). Furthermore, the previous studies have showed that rpoS is involved in biofilm production in many bacteria, and biofilm formation could enhance the resistance to natural environment stresses and host immune killing ( Kulikalova et al, 2014 ; Beshiru and Igbinosa, 2018 ; Olwal et al, 2018 ). Our studies also showed that knockdown of rpoS could decrease the biofilm production in E. cloacae , which demonstrated tha t rpoS plays an important role in E. cloacae biofilm production.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii

Gao

Qian²,

Zhu³

et al. 2022

Front. Microbiol.

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Enterobacter cloacae is widely distributed in the aquatic environment, and has been determined as a novel pathogen of various aquatic animals recently. Our previous studies have indicated E. cloacae caused repeated infections in Macrobrachium rosenbergii, suggesting a high survival ability of the bacteria, and rpoS gene has been known to regulate stress response and virulence of many bacteria. In this study, the E. cloacae-rpoS RNAi strain was constructed by RNAi technology, and the regulation role of rpoS in stress resistance and virulence of E. cloacae was explored by transcriptomic and phenotype analysis. The transcriptome analysis showed a total of 488 differentially expressed genes (DEGs) were identified between rpoS-RNAi and wild-type strains, including 30 up-regulated genes and 458 down-regulated genes, and these down-regulated DEGs were mainly related to environmental response, biofilm formation, bacterial type II secretory system, flagellin, fimbrillin, and chemotactic protein which associated with bacterial survival and virulence. The phenotype changes also showed the E. cloacae-rpoS RNAi strain exhibited significantly decreasing abilities of survival in environmental stresses (starvation, salinity, low pH, and oxidative stress), biofilm production, movement, adhesion to cells, pathogenicity, and colonization to M. rosenbergii. These results reveal that rpoS plays an important regulatory role in environmental stress adaptation and virulence of E. cloacae.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii

Gao

Qian²,

Zhu³

et al. 2022

Front. Microbiol.

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“…In addition to immunological, microbiological and demographic factors, numerous factors (including but not limited to the geographical environment) may also influence the development and progression of epidemics. To explore such factors, the analysis of variables that can be defined in terms of geographical coordinates (geo-referenced variables) has been proposed (1)(2)(3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

Assessing complexity and dynamics in epidemics: geographical barriers and facilitators of foot-and-mouth disease dissemination

Hoogesteyn

Rivas

Smith³

et al. 2023

Front. Vet. Sci.

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IntroductionPhysical and non-physical processes that occur in nature may influence biological processes, such as dissemination of infectious diseases. However, such processes may be hard to detect when they are complex systems. Because complexity is a dynamic and non-linear interaction among numerous elements and structural levels in which specific effects are not necessarily linked to any one specific element, cause-effect connections are rarely or poorly observed.MethodsTo test this hypothesis, the complex and dynamic properties of geo-biological data were explored with high-resolution epidemiological data collected in the 2001 Uruguayan foot-and-mouth disease (FMD) epizootic that mainly affected cattle. County-level data on cases, farm density, road density, river density, and the ratio of road (or river) length/county perimeter were analyzed with an open-ended procedure that identified geographical clustering in the first 11 epidemic weeks. Two questions were asked: (i) do geo-referenced epidemiologic data display complex properties? and (ii) can such properties facilitate or prevent disease dissemination?ResultsEmergent patterns were detected when complex data structures were analyzed, which were not observed when variables were assessed individually. Complex properties–including data circularity–were demonstrated. The emergent patterns helped identify 11 counties as ‘disseminators’ or ‘facilitators’ (F) and 264 counties as ‘barriers’ (B) of epidemic spread. In the early epidemic phase, F and B counties differed in terms of road density and FMD case density. Focusing on non-biological, geographical data, a second analysis indicated that complex relationships may identify B-like counties even before epidemics occur.DiscussionGeographical barriers and/or promoters of disease dispersal may precede the introduction of emerging pathogens. If corroborated, the analysis of geo-referenced complexity may support anticipatory epidemiological policies.

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Model of Vibrio cholerae biofilm as a mechanism of its survival in surface water reservoirs

Cited by 2 publications

References 20 publications

Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii

Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii

Assessing complexity and dynamics in epidemics: geographical barriers and facilitators of foot-and-mouth disease dissemination

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