Model of immunogenic uveitis in rabbits

Нероев, В. В.; Давыдова, Г. А.; Perova, T S

doi:10.1007/s10517-006-0440-5

Cited by 7 publications

(3 citation statements)

References 2 publications

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“…Immunogenic uveitis was induced as described in [ 24 ]. Briefly, rabbits were initially injected subcutaneously with 5 mL of normal horse serum for sensitization.…”

Section: Methodsmentioning

confidence: 99%

Superoxide Dismutase 1 Nanozyme for Treatment of Eye Inflammation

Кост

Безнос

Davydova

et al. 2015

Oxidative Medicine and Cellular Longevity

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Use of antioxidants to mitigate oxidative stress during ocular inflammatory diseases has shown therapeutic potential. This work examines a nanoscale therapeutic modality for the eye on the base of antioxidant enzyme, superoxide dismutase 1 (SOD1), termed “nanozyme.” The nanozyme is produced by electrostatic coupling of the SOD1 with a cationic block copolymer, poly(L-lysine)-poly(ethyleneglycol), followed by covalent cross-linking of the complexes with 3,3′-dithiobis(sulfosuccinimidylpropionate) sodium salt. The ability of SOD1 nanozyme as well as the native SOD1 to reduce inflammatory processes in the eye was examined in vivo in rabbits with immunogenic uveitis. Results suggested that topical instillations of both enzyme forms demonstrated anti-inflammatory activity; however, the nanozyme was much more effective compared to the free enzyme in decreasing uveitis manifestations. In particular, we noted statistically significant differences in such inflammatory signs in the eye as the intensities of corneal and iris edema, hyperemia of conjunctiva, lens opacity, fibrin clots, and the protein content in aqueous humor. Clinical findings were confirmed by histological data. Thus, SOD1-containing nanozyme is potentially useful therapeutic agent for the treatment of ocular inflammatory disorders.

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“…Immunogenic uveitis was induced as described in [ 24 ]. Briefly, rabbits were initially injected subcutaneously with 5 mL of normal horse serum for sensitization.…”

Section: Methodsmentioning

confidence: 99%

Superoxide Dismutase 1 Nanozyme for Treatment of Eye Inflammation

Кост

Безнос

Davydova

et al. 2015

Oxidative Medicine and Cellular Longevity

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“…Моделирование экспериментального иммуногенного увеита у кроликов В эксперименте было задействовано 15 кроликов породы шиншилла массой 2-2,5 кг. У 10 животных воспроизводили иммуногенный увеит по известной методике [22]. Для этого животным вводили стерильную нормальную лошадиную сыворотку подкожно в количестве 5 мл, затем на 10-е сутки в оба глаза интравитреально вводили разрешающую дозу сыворотки в количестве 70 мкл под местной анестезией Алкаином (Проксиметакаин 0,5 %, Alcon, Бельгия).…”

Section: получение кфчunclassified

Calcium-Phosphate Nanoparticles — a System for Drug Delivery to the Anterior Eye Chamber

et al. 2021

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Purpose: to prepare and characterize calcium-phosphate nanoparticles loaded with compounds of different nature: low-molecular inhibitor of angiotensin-converting enzyme lisinopril, and high-molecular enzyme superoxide dismutase 1. To estimate the possibility of enhancing the biological efficacy of these compounds via incorporation to the nanoparticles.Material and methods. To increase the stability of calcium-phosphate nanoparticles coating with β-D-cellobiose was used. The size, surface charge (ζ-potential) of the particles and efficacy of including of the selected compounds to the particles were measured. Comparative assessment of the efficacy of lisinopril solution and lisinopril in nanoparticles was made via the estimation of their ocular hypotensive effect in normotensive rabbits. To compare the efficacy of the superoxide dismutase 1 solution and superoxide dismutase 1 in nanoparticles the rabbit model of immunogenic uveitis was used. We estimated the clinical score for several signs of uveitis, protein level, and antioxidant activity in aqueous humor.Results. Calcium-phosphate nanoparticles containing lisinopril had average hydrodynamic radius of 170–300 nm and negative ζ-potential of –17 mV. Particles containing superoxide dismutase 1 had average hydrodynamic radius of 220–450 nm and negative ζ-potential of –4 mV. Lisinopril in nanoparticles caused a significantly greater decrease of intraocular pressure than lisinopril solution. Superoxide dismutase 1 in calcium-phosphate nanoparticles more efficiently decreased the clinical manifestations of uveitis and normalized the biochemical processes in aqueous humor than the enzyme in buffer solution.Conclusion. Incorporation of both low-molecular and high-molecular drugs to the calcium-phosphate nanoparticles enhance their bioavailability and therapeutic efficiency. The data obtained give evidence of the prospectively of the using of these nanoparticles as vehicles for the ophthalmic drugs used in eyedrops.

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“…Работа выполнена на 20 кроликах (40 глаз) породы шиншилла массой 2-2,5 кг. У 16 животных воспроизводили увеит путем двукратного введения нормальной лошадиной сыворотки: первую дозу (5 мл) вводили подкожно, вторую (0,07 мл) -интравитреально на 10-е сутки после первой [5]. Для контроля биохимических показателей были взяты 4 здоровых кролика (8 глаз).…”

Section: материал и методыunclassified

Effects of dexamethasone and superoxide dismutase instillations on clinical course of uveitis and local biochemical processes (experimental study)

Безнос

et al. 2015

Vestn. oftal'mol.

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The effects of SOD and dexamethasone instillations were considered similar in many parameters. However, SOD was associated with a greater increase in antioxidant activity and a greater decrease in aqueous humor leukocytes, while dexamethasone was more effective in decreasing aqueous humor α2-macroglobulin and vitreous humor protein and albumin. The substances had a synergistic effect on iridal edema as well as aqueous humor leukocyte number and α2-macroglobulin level. CONCLUSION. Adding SOD to the complex therapy of uveitis results in lower inflammation intensity and enhanced dexamethasone effect.

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Model of immunogenic uveitis in rabbits

Cited by 7 publications

References 2 publications

Superoxide Dismutase 1 Nanozyme for Treatment of Eye Inflammation

Superoxide Dismutase 1 Nanozyme for Treatment of Eye Inflammation

Calcium-Phosphate Nanoparticles — a System for Drug Delivery to the Anterior Eye Chamber

Effects of dexamethasone and superoxide dismutase instillations on clinical course of uveitis and local biochemical processes (experimental study)

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