Model of a Raft in Both Leaves of an Asymmetric Lipid Bilayer

Shlomovitz, Roie; Schick, M.

doi:10.1016/j.bpj.2013.06.053

Cited by 57 publications

(94 citation statements)

References 52 publications

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“…These effects can be captured in models with not one, but two composition fields (one for each leaflet). Coupling of these fields gives rise to complex phenomena that are currently being explored [51, 52]. …”

Section: Discussion and Outlookmentioning

confidence: 99%

Seeing the Forest in Lieu of the Trees

Sapp

Shlomovitz

Maibaum

2014

Annual Reports in Computational Chemistry

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Biological membranes exhibit long-range spatial structure in both chemical composition and geometric shape, which gives rise to remarkable physical phenomena and important biological functions. Continuum models that describe these effects play an important role in our understanding of membrane biophysics at large length scales. We review the mathematical framework used to describe both composition and shape degrees of freedom, and present best practices to implement such models in a computer simulation. We discuss in detail two applications of continuum models of cell membranes: the formation of microemulsion and modulated phases, and the effect of membrane-mediated interactions on the assembly of membrane proteins.

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Section: Discussion and Outlookmentioning

confidence: 99%

Seeing the Forest in Lieu of the Trees

Sapp

Shlomovitz

Maibaum

2014

Annual Reports in Computational Chemistry

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“…In view of raft formation, the coupling between membrane lipid composition and local curvature is currently attracting much attention [30,31,40]. It has been shown that such a coupling is able to induce composition fluctuations on a length scale of 10-100 nm, which is compatible with the size of rafts.…”

Section: Discussionmentioning

confidence: 99%

“…We emphasize that the formation of anti-correlated domains requires the composition to be the same in both leaflets [30]. In case this condition is not met, the coupling between the leaflets is more complex, and also correlated domains are possible [31].…”

Section: Membrane "Sandwiched" Between a Solid Substrate And An Actinmentioning

confidence: 99%

“…The pinning sites along the actin fibers locally push the membrane down, leading to non-zero average curvature below the fibers. Consider now a lipid mixture, with one of the lipid species preferring regions of, say, positive curvature (the coupling between membrane composition and curvature is an established fact [30][31][32][33][34][35]). In the upper membrane leaflet, these lipids would then preferentially collect underneath the actin fibers, since there the curvature has the correct sign.…”

Section: Membrane "Sandwiched" Between a Solid Substrate And An Actinmentioning

confidence: 99%

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Membrane sorting via the extracellular matrix

Sadeghi

Vink

2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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We consider the coupling between a membrane and the extracellular matrix. Computer simulations demonstrate that the latter coupling is able to sort lipids. It is assumed that membranes are elastic manifolds, and that this manifold is disrupted by the extracellular matrix. For a solid-supported membrane with an actin network on top, regions of positive curvature are induced below the actin fibers. A similar mechanism is conceivable by assuming that the proteins which connect the cytoskeleton to the membrane induce local membrane curvature. The regions of non-zero curvature exist irrespective of any phase transition the lipids themselves may undergo. For lipids that prefer certain curvature, the extracellular matrix thus provides a spatial template for the resulting lateral domain structure of the membrane.

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“…Indeed, lipid-lipid interactions were capable of inducing liquid ordered (Lo)-liquid disordered (Ld) phase coexistence in model lipid membranes [55,56]. It was conjectured that plasma membrane composition is poised for selective and functional raft clustering at physiological temperatures [57].…”

Section: Nonraft Lipids and Sphingolipids In Live Plasma Membranes Sementioning

confidence: 99%

Biomolecule-Conjugated Quantum Dot Nanosensors as Probes for Cellular Dynamic Events in Living Cells

Huang¹

2018

Nonmagnetic and Magnetic Quantum Dots

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A single-molecule tracking/imaging technique with semiconductor quantum dot (QD) nanosensors conjugated with appropriate peptides or antibodies is appealing for probing cellular dynamic events in living cells. We developed a 2D analysis of singlemolecule trajectories using normalized variance versus mean square displacement (MSD) to provide high-quality statistics sampled by nanosensors while preserving single-molecule sensitivity. This plot can be more informative than MSD alone to reflect the diffusive dynamics of a protein in its cellular environment. We illustrate the performance of this technique with selected examples, which are designed to expose the functionalities and importance in live cells. Our findings suggest that biomoleculeconjugated QD nanosensors can be used to reveal interactions, stoichiometries, and conformations of proteins, and provide an understanding of the mode of the interaction, stable states, and dynamical pathways of biomolecules in live cells.

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Model of a Raft in Both Leaves of an Asymmetric Lipid Bilayer

Cited by 57 publications

References 52 publications

Seeing the Forest in Lieu of the Trees

Seeing the Forest in Lieu of the Trees

Membrane sorting via the extracellular matrix

Biomolecule-Conjugated Quantum Dot Nanosensors as Probes for Cellular Dynamic Events in Living Cells

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