Model of a Plasmonic Phase Interrogation Probe for Optical Sensing of Hemoglobin in Blood Samples

Abstract

Phase interrogation based surface plasmon resonance (SPR) biosensor is proposed for the determination of Hb concentration. Previous experimental results describing variation of refractive index of human blood with Hb concentration at different wavelengths are considered for design simulations. The biosensor design with silica substrate and gold layer is considered. The sensor's performance is closely analyzed in terms of phase sensitivity and resolution. The influence of operating wavelength on biosensor's performance for Hb measurement is critically investigated, which points to carry out the Hb measurement at a shorter wavelength as phase sensitivity and resolution increase significantly with decrease in wavelength. The results are explained in terms of suitable physical concepts such as radiation damping. Use of contamination-preventing biochemical layer ascertains the stability of measurement with the intended SPR biosensor probe. The simulation results also highlight that the resolution of Hb measurement achievable with the proposed biosensor is much higher compared with several existing methods.

Analysis of Coupled Surface Plasmon in LHM Mediated Dielectric Gap Multilayer Structure

“…To produce a sharp and sensitive peak ascribed to coupled modes, Kullab et al [26] proposed a novel four-layer scheme where a metamaterial (left-handed material and right-handed material) is included as a core layer to demonstrate that the designed structure presents an advantage over other optical sensors. It should be noted that the largest peak of the resonance condition, measured on angular reflectance spectra, constitutes a disadvantage for using the resonance angle as a key parameter in characterizing RI of aqueous solutions on the SPR sensors' surface [27,28].…”