Model based analysis of transient fluorescence yield induced by actinic laser flashes in spinach leaves and cells of green alga Chlorella pyrenoidosa Chick

Belyaeva, N. E.; Schmitt, Franz‐Josef; Paschenko, V.Z.; Riznichenko, G. Yu.; Rubin, A. B.; Ренгер, Г.

doi:10.1016/j.plaphy.2014.01.017

Cited by 21 publications

(24 citation statements)

References 41 publications

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“…In the present work the PSII model specified a fraction of up to 25 % of S 1 Q À B semiquinone in the dark imitating the known results of (Rutherford et al 1982;de Wijn and van Gorkom 2001;Cardona et al 2012) to fit the distinctive measured SFITFY patterns (Steffen et al 2005;Belyaeva et al 2014). The SFITFY rise up to about 10 ls was shown to be determined strongly by the actinic flash energy responsible for the transient PSII redox state dynamics starting on the picosecond time scale.…”

Section: Introductionmentioning

confidence: 80%

“…These time constants diminish from tens of ns to tens of ls (k WOCmin ) to describe the reduction of Y OX Z in the S 1 ? S 2 WOC transition (Kunn et al 2004;Renger 2004Renger , 2012Belyaeva et al 2014). The electron donation Y Z ?…”

Section: Methods For Data Interpretationmentioning

confidence: 99%

“…In particular, analytical functions were proposed to describe (a) the transient 3 Car(t) population (Belyaeva et al 2008) vanishing on the microsecond time scale after a pulse excitation; (b) the dynamics of the chlorophyll excitation by the single turnover flash followed by non actinic measuring light with a low intensity (Belyaeva et al 2011a); (c) the light-induced time courses of DW, pH Lumen , and pH Stroma (Belyaeva et al 2011b); (d) the multiphasic nanosecond kinetics of the ET from Y Z to P þ 680 followed by an electron donation from WOC to Y Z coupled to the transition S 1 ? S 2 (Belyaeva et al 2014). …”

Section: Introductionmentioning

confidence: 96%

“…In the present study, the PSII model is suitable to analyze the ET and PT in PSII by fitting of SFITFY data measured before on samples of leaves and thermophylic alga cells (Steffen 2003;Steffen et al 2005;Belyaeva et al 2008Belyaeva et al , 2014. For the first time, the transient dynamics of the population of all involved RC states are visualized for the studied species over the wide time scale to display the electron pathway in the RCs as a result of the short actinic flash followed by the spontaneous relaxation dynamics of the electrons.…”

Section: Introductionmentioning

confidence: 99%

“…The SFITFY rise up to about 10 ls was shown to be determined strongly by the actinic flash energy responsible for the transient PSII redox state dynamics starting on the picosecond time scale. Meanwhile the hypothetical effect of the slower ET from Q À A to Q ðÀÞ B (Belyaeva et al 2014) was kept as an explanation for the strong differences observed for the SFITFY curves in leaves and C. pyrenoidosa Chick cells on the time scale of seconds. This is displayed for the studied species in the work shown here.…”

Section: Introductionmentioning

confidence: 99%

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Modeling of the redox state dynamics in photosystem II of Chlorella pyrenoidosa Chick cells and leaves of spinach and Arabidopsis thaliana from single flash-induced fluorescence quantum yield changes on the 100 ns–10 s time scale

Belyaeva

Schmitt²,

Paschenko

et al. 2015

Photosynth Res

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The time courses of the photosystem II (PSII) redox states were analyzed with a model scheme supposing a fraction of 11-25 % semiquinone (with reduced [Formula: see text]) RCs in the dark. Patterns of single flash-induced transient fluorescence yield (SFITFY) measured for leaves (spinach and Arabidopsis (A.) thaliana) and the thermophilic alga Chlorella (C.) pyrenoidosa Chick (Steffen et al. Biochemistry 44:3123-3132, 2005; Belyaeva et al. Photosynth Res 98:105-119, 2008, Plant Physiol Biochem 77:49-59, 2014) were fitted with the PSII model. The simulations show that at high-light conditions the flash generated triplet carotenoid (3)Car(t) population is the main NPQ regulator decaying in the time interval of 6-8 μs. So the SFITFY increase up to the maximum level [Formula: see text]/F 0 (at ~50 μs) depends mainly on the flash energy. Transient electron redistributions on the RC redox cofactors were displayed to explain the SFITFY measured by weak light pulses during the PSII relaxation by electron transfer (ET) steps and coupled proton transfer on both the donor and the acceptor side of the PSII. The contribution of non-radiative charge recombination was taken into account. Analytical expressions for the laser flash, the (3)Car(t) decay and the work of the water-oxidizing complex (WOC) were used to improve the modeled P680(+) reduction by YZ in the state S 1 of the WOC. All parameter values were compared between spinach, A. thaliana leaves and C. pyrenoidosa alga cells and at different laser flash energies. ET from [Formula: see text] slower in alga as compared to leaf samples was elucidated by the dynamics of [Formula: see text] fractions to fit SFITFY data. Low membrane energization after the 10 ns single turnover flash was modeled: the ∆Ψ(t) amplitude (20 mV) is found to be about 5-fold smaller than under the continuous light induction; the time-independent lumen pHL, stroma pHS are fitted close to dark estimates. Depending on the flash energy used at 1.4, 4, 100 % the pHS in stroma is fitted to 7.3, 7.4, and 7.7, respectively. The biggest ∆pH difference between stroma and lumen was found to be 1.2, thus pH- dependent NPQ was not considered.

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