Model Affitin and PEG modifications onto siRNA lipid nanocapsules: cell uptake and <i>in vivo</i> biodistribution improvements

Resnier, Pauline; Lepeltier, Élise; Emina, Anthea Lucrezia; Galopin, Natacha; Bejaud, Jérôme; David, Stéphanie; Ballet, Caroline; Benvegnu, Thierry; Pecorari, Frédéric; Chourpa, Igor; Benoı̂t, Jean-Pierre; Passirani, Catherine

doi:10.1039/c9ra03668g

Cited by 13 publications

(6 citation statements)

References 56 publications

(80 reference statements)

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“…The Benvegnu group has also worked extensively in the area of synthetic BA archaeasomes (Benvegnu et al, 2005 ; Jiblaoui et al, 2016 ; Resnier et al, 2019 ). In 2016 (Jiblaoui et al, 2016 ), they reported the synthesis and characterization of folate-conjugated archaeosomes (see Figure 5 ).…”

Section: Bolaamphiphile Specific Considerations Relevant To Biomedicamentioning

confidence: 99%

Biomedically Relevant Applications of Bolaamphiphiles and Bolaamphiphile-Containing Materials

et al. 2021

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Bolaamphiphiles (BAs) are structurally segmented molecules with rich assembly characteristics and diverse physical properties. Interest in BAs as standalone active agents or as constituents of more complex therapeutic formulations has increased substantially in recent years. The preorganized amphiphilicity of BAs allows for a range of biological activities including applications that rely on multivalency. This review summarizes BA-related research in biomedically relevant areas. In particular, we review BA-related literature in four areas: gene delivery, antimicrobial materials, hydrogels, and prodrugs. We also discuss several distinguishing characteristics of BAs that impact their utility as biomedically relevant compounds.

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Section: Bolaamphiphile Specific Considerations Relevant To Biomedicamentioning

confidence: 99%

Biomedically Relevant Applications of Bolaamphiphiles and Bolaamphiphile-Containing Materials

et al. 2021

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“…Our data suggest that, due to our formulation strategy, pH-sensitive nanovectors would be able to reverse MDR phenotype to improve cancer treatment. Furthermore, as previously studied by our group [57,[63][64][65], different post-insertion strategies applied to LNC offer prospects for obtaining conclusive results about the effective ability of these nanoparticles to be loaded with the substances of interest, such as DNA, RNA or other drugs as ferrocifens.…”

Section: Discussionmentioning

confidence: 93%

pH-Responsive Lipid Nanocapsules: A Promising Strategy for Improved Resistant Melanoma Cell Internalization

Pautu

Lepeltier

Mellinger

et al. 2021

Cancers

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Despite significant advances in melanoma therapy, low response rates and multidrug resistance (MDR) have been described, reducing the anticancer efficacy of the administered molecules. Among the causes to explain these resistances, the decreased intratumoral pH is known to potentiate MDR and to reduce the sensitivity to anticancer molecules. Nanomedicines have been widely exploited as the carriers of MDR reversing molecules. Lipid nanocapsules (LNC) are nanoparticles that have already demonstrated their ability to improve cancer treatment. Here, LNC were modified with novel copolymers that combine N-vinylpyrrolidone (NVP) to impart stealth properties and vinyl imidazole (Vim), providing pH-responsive ability to address classical chemoresistance by improving tumor cell entry. These copolymers could be post-inserted at the LNC surface, leading to the property of going from neutral charge under physiological pH to positive charge under acidic conditions. LNC modified with polymer P5 (C18H37-P(NVP21-co-Vim15)) showed in vitro pH-responsive properties characterized by an enhanced cellular uptake under acidic conditions. Moreover, P5 surface modification led to an increased biological effect by protecting the nanocarrier from opsonization by complement activation. These data suggest that pH-sensitive LNC responds to what is expected from a promising nanocarrier to target metastatic melanoma.

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“…2 ). A number of studies focusing on the engineering of nanoparticles for targeted delivery based on repebodies [ 49 , 50 , 51 ], affimers [ 52 ], affitins [ 53 , 54 , 55 ], and knottins [ 56 ] have also been conducted.…”

Section: The Main Structural Characteristics Of Scaffold Proteins And...mentioning

confidence: 99%

Artificial Scaffold PolypeptidesAs an Efficient Tool for the Targeted Delivery of Nanostructures In Vitro and In Vivo

Shipunova

Deyev

2022

Acta Naturae

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The use of traditional tools for the targeted delivery of nanostructures, such as antibodies, transferrin, lectins, or aptamers, often leads to an entire range of undesirable effects. The large size of antibodies often does not allow one to reach the required number of molecules on the surface of nanostructures during modification, and the constant domains of heavy chains, due to their effector functions, can induce phagocytosis. In the recent two decades, targeted polypeptide scaffold molecules of a non-immunoglobulin nature, antibody mimetics, have emerged as much more effective targeting tools. They are small in size (320 kDa), possess high affinity (from subnano- to femtomolar binding constants), low immunogenicity, and exceptional thermodynamic stability. These molecules can be effectively produced in bacterial cells, and, using genetic engineering manipulations, it is possible to create multispecific fusion proteins for the targeting of nanoparticles to cells with a given molecular portrait, which makes scaffold polypeptides an optimal tool for theranostics.

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Model Affitin and PEG modifications onto siRNA lipid nanocapsules: cell uptake and in vivo biodistribution improvements

Abstract: Surface modifications of siRNA LNCs were assessed with innovative TE-PEG polymers and an Affitin model, in comparison to classic DSPE-PEG LNCs, in order to evaluate the potential tumor targeting of siRNA after intravenous administration.

Cited by 13 publications

References 56 publications

Biomedically Relevant Applications of Bolaamphiphiles and Bolaamphiphile-Containing Materials

Biomedically Relevant Applications of Bolaamphiphiles and Bolaamphiphile-Containing Materials

pH-Responsive Lipid Nanocapsules: A Promising Strategy for Improved Resistant Melanoma Cell Internalization

Artificial Scaffold PolypeptidesAs an Efficient Tool for the Targeted Delivery of Nanostructures In Vitro and In Vivo

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