Mode of Subacute Sclerosing Panencephalitis (SSPE) Virus Infection in Tissue Culture Cells: III. Neurovirulence of Cell‐Free SSPE Viruses of Niigata‐1, Kitaken‐1, and Biken Strains

Ohuchi, Mayuko; Ohuchi, Reiko; Homma, M.

doi:10.1111/j.1348-0421.1981.tb00093.x

Cited by 12 publications

(12 citation statements)

References 22 publications

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“…The reason for the poor viral recovery from mice infected with the Biken strain is still unknown. In the tissue culture system, the Biken strain hardly pro ducedcell-free virus (9) and hemadsorption of the infected cells was negative (un publisheddata).…”

Section: Discussionmentioning

confidence: 99%

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Slow Development of Measles Virus (Edmonston Strain) Infection in the Brain of Nude Mice

Ohuchi¹,

Ohuchi²,

Mifune³

1984

Microbiology and Immunology

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The Edmonston strain of measles virus caused neurologic disease in athymic nude mice by intracerebral inoculation. The incubation periods of the disease, however, were extremely long, ranging from 59 to 140 days when the mice were inoculated with 104 plaque forming units (PFU) of the virus. The Edmonston strain was highly infectious in the nude mouse brain since virus infection was established even with 1 PFU of the virus. Virus titers in the brains of infected mice increased with the time of incubation. These results indicate that the extremely long incubation period of the disease is ascribed to very slow development of virus infection in the mouse brain. On the other hand, the incubation periods of the Biken strain of SSPE virus were very short (generally within 2 weeks) even with inoculations of 1 PFU of the virus. However, the extent of the dissemination of infection in brains was not significantly different between the two viruses as examined by immunofluorescent staining.

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Section: Discussionmentioning

confidence: 99%

“…The cells were grown at 36 C in Eagle's minimum essential medium (MEM) supplemented with 10% bovine serum. The cell-free virus was prepared by freeze-thawing of the infected cultures at the 19th to 21st passage as described previously (9).…”

mentioning

confidence: 99%

Slow Development of Measles Virus (Edmonston Strain) Infection in the Brain of Nude Mice

Ohuchi¹,

Ohuchi²,

Mifune³

1984

Microbiology and Immunology

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“…The Biken strain of defective SSPE virus shows neurovirulence in mice and hamsters at low passage levels (Thormar et al, 1978;Ohuchi et al, 1981), but its neurovirulence in hamsters has been found to be decreased after passage for more than 100 generations in HEL cells (S. Ueda, personal communication). In this study, defective SSPE virus which had lost its neurovirulence during passages in HEL cells was transferred to three human neural cell lines.…”

Section: Laboratory Animal Research Center Institute Of Medical Sciementioning

confidence: 99%

Growth of Defective Subacute Sclerosing Panencephalitis Viruses in Human Neural Cells and Their Neurovirulence in Mice

Sakaguchi

Yoshikawa

Yamanouchi

et al. 1985

Journal of General Virology

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SUMMARYA defective subacute sclerosing panencephalitis (SSPE) virus which had been passaged in human embryonic lung cells was transferred to cultures of three neural cell types: neuroblastoma, oligodendroglioma and glioblastoma. The growth characteristics of the virus in these cells were essentially similar to those in non-neural cells. On the other hand, a marked difference in neurovirulence was noticed for the virus grown in neural cells when examined by intracerebral inoculation into mice. The virus passaged in neuroblastoma and oligodendroglioma cells showed high neurovirulence, inducing an acute encephalitis, whereas the virus passaged in human embryonic lung cells and that in glioblastoma cells did not show neurovirulence. These results suggest that the virus recovered its neurovirulence after passages in certain human neural cells.

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“…Nevertheless, cell-free virus-like particles are needed to study their cell tropism for different cell lines. The freeze-thawing method (43) was unacceptable because our strains could not be reproducibly prepared and the titers were unsatisfactory. Preparation by treating infected cells with cytochalasin D (CD-VLPs) (2) enabled us to infect cell lines and thereby compare the infectivity differences between SSPE virus and MV.…”

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confidence: 99%

Infection of Different Cell Lines of Neural Origin with Subacute Sclerosing Panencephalitis (SSPE) Virus

Ishida

Ayata

Shingai

et al. 2004

Microbiology and Immunology

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Measles virus is the causative agent of subacute sclerosing panencephalitis (SSPE). The viruses isolated from brain cells of patients with SSPE (called SSPE viruses) are defective in cell‐free virus production in vitro. To investigate the cell tropism of three strains of SSPE virus (Osaka‐1, Osaka‐2, Osaka‐3), SSPE virus‐infected cell cultures were treated with cytochalasin D to prepare virus‐like particles (CD‐VLPs). All CD‐VLPs formed syncytia after infection in CHO cells expressing CD150 but not in those expressing CD46. In addition, an antibody to CD46 did not block the infection of Vero cells by SSPE CD‐VLPs. The results were consistent with our previous suggestion that one or more unidentified receptors might be involved in the entry process. Infection with the CD‐VLPs from three SSPE strains was further examined in different human cell lines, including those of neural origin, and was found to induce syncytia in epithelial cells (HeLa and 293T) as well as neuroblastoma cells (IMR‐32 and SK‐N‐SH) with varying efficiency. SSPE CD‐VLPs also infected glioblastoma cells (A172) and astrocytoma cells (U‐251) but syncytial formation was rarely induced. These epithelial and neural cell lines were not permissive for the replication of wild‐type MV. Together with our previous observations, these results suggest that the cell entry receptor is the major factor determining the cell tropism of SSPE viruses. Further studies are necessary to identify other viral and/or cellular factors that might be involved in the replication of SSPE virus in specific neural cells and in the brain.

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Mode of Subacute Sclerosing Panencephalitis (SSPE) Virus Infection in Tissue Culture Cells: III. Neurovirulence of Cell‐Free SSPE Viruses of Niigata‐1, Kitaken‐1, and Biken Strains

Cited by 12 publications

References 22 publications

Slow Development of Measles Virus (Edmonston Strain) Infection in the Brain of Nude Mice

Slow Development of Measles Virus (Edmonston Strain) Infection in the Brain of Nude Mice

Growth of Defective Subacute Sclerosing Panencephalitis Viruses in Human Neural Cells and Their Neurovirulence in Mice

Infection of Different Cell Lines of Neural Origin with Subacute Sclerosing Panencephalitis (SSPE) Virus

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