1957
DOI: 10.1507/endocrj1954.4.236
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MODE OF ACTION OF THYROXINE UPON SUCCINIC DEHYDROGENASE SYSTEM OF RAT TISSUE HOMOGENATES <I>IN VITRO</I>

Abstract: Processes of methylene blue reduction as affected by change in degree of evacuation.In this case,0.4ml of 0.5% mouse liver homogenate (2 mg wet weight/3 nil),0.2ml of 0.2 M succinate and 0.2ml of 0.01% methylene blue were used.Degree of evacuation in A:for 3 minutes to 7 mmHa,B:for 6 minutes to 7 mmHg,C: for 10 minutes to 6 mmHg, D:for 20 minutes to 5.5 mmHg(duplicate). YAMAMOTO

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“…The activity of succinic dehydrogenase was measured employing Thunberg tubes after Yamamoto and Sugisawa (11). The composition of the reaction mixture was as follows:In the main tube:1) 0.1M K2HPO4-KH2PO4 buffer (PH 7.4) 1.3 ml., 2) 0.2% homogenate 0.6ml., 3) fig.…”
Section: Methodsmentioning
confidence: 99%
“…The activity of succinic dehydrogenase was measured employing Thunberg tubes after Yamamoto and Sugisawa (11). The composition of the reaction mixture was as follows:In the main tube:1) 0.1M K2HPO4-KH2PO4 buffer (PH 7.4) 1.3 ml., 2) 0.2% homogenate 0.6ml., 3) fig.…”
Section: Methodsmentioning
confidence: 99%
“…p. m. for 2 mins. , succinic and glutamic dehydrogenases were assayed as described before (Yamamoto and Sugisawa, 1957). The table shows comparative activities of the two enzymes.…”
mentioning
confidence: 99%