Mode of action of pentocin 31-1: An antilisteria bacteriocin produced by Lactobacillus pentosus from Chinese traditional ham

Zhou, Kang; Zhou, Wei; Li, Pinglan; Liu, Guorong; Zhang, Jinlan; Dai, Yunqing

doi:10.1016/j.foodcont.2007.08.008

Cited by 99 publications

(68 citation statements)

References 25 publications

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“…sake, L. pentosusetc (7,24,25). The antibacterial potential of these bacteria havebeen attributed to their ability to produce H 2 O 2 , acids, phages, bacteriocins and bacteriocin like substances (26).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Partially Purified Bacteriocin Like Substance (BLIS) Produced by Probiotic Lactobacillus Strains

Khanian¹,

Mojgani²,

Ahmedi³

2014

Int J Enteric Pathog

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Background:There is an increasing interest in search for antimicrobial peptides (bacteriocins and bacteriocin-like compounds) produced by lactic acid bacteria (LAB) because of their potential to be used as antimicrobial agents for improving the safety of food products. Objectives: The main objective of study was to evaluate the antibacterial potential of locally isolated Lactic Acid bacteria (LAB) and determine their bacteriocin producing ability in in-vitro conditions. Materials and Methods: The antibacterial activity of 77 isolated LAB strains was tested against a number of pathogens by well-diffusion method. The isolates demonstrating antimicrobial potential were selected and tested for the production of bacteriocin or bacteriocin like substance. The bacteriocin produced by two of the isolates were partially purified and characterized. Results:The results indicated the neutralized supernatant fluid of two of the isolates identified as L. brevis LB32 and L. pentosus LP05, were active against the growth of Listeria monocytogenes, Salmonella enteritidis, Shigella dysenteriae, Staphylococcus aureus and Streptococcus pneumoniae. Additionally, L. brevis LB32 was able to inhibit the growth of Salmonella typhi and Klebsiella pneumoniae, while, S. pnuemoniae and L. monocytogenes appeared to be the most sensitive strain as apparent by highest zone of inhibition against these pathogens, respectively. The antimicrobial activity in the supernatant fluids of the mentioned strains remained unaffected after treating with enzymes catalase, lipase and lysozyme, while were strongly sensitive to the action of proteolytic enzymes, suggesting the presence of bacteriocin like inhibitory substance (BLIS) in the two isolates. The inhibitory substance produced by the two isolates appeared heat resistant and tolerated 100˚C and 121˚C for 55 minutes and 20 minutes, respectively. Partial purification of the concentrated culture supernatant fluids of L. brevis LB32 and L. pentosus LP05 by ammonium sulphate (80%) and DEAE cellulose columns resulted in an enhanced activity (AU/mL) and yield. Using different pore size ultra filter membranes and SDS-PAGE analysis, the approximate molecular weight of the BLIS produced by L. brevis LB32 and, L. pentosus LP05 appeared to be approximately 4.5 and 6 KDa, respectively. In contrast to L. brevis LB32, L. pentosus LP05 harbored an 18Kb plasmid DNA which appeared to be carrying the bacteriocin gene as evident by plasmid curing experiments. All the mutants retained their host immunity and were resistant to the bacteriocin produced by the parent strain. Conclusions:In conclusion, the antibacterial activity possessed by these isolates might be used for the control of unwanted pathogens mainly in dairy products, and could be investigated further for using in fermented dairy products.

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Section: Discussionmentioning

confidence: 99%

Characterization of Partially Purified Bacteriocin Like Substance (BLIS) Produced by Probiotic Lactobacillus Strains

Khanian¹,

Mojgani²,

Ahmedi³

2014

Int J Enteric Pathog

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“…Leakage assay. The spores of B. subtilis and B. licheniformis (2 × 10 8 spore/ml) were combined with glycinin, BS or penicillin at different concentrations (100, 200, 300 and 400 µg/ml) before incubating at 35°C for 2 h. The concentration of the UV-absorbing material (OD 260 ) released from treated germinating spores was measured using a JENWAY 6405 UK spectrophotometer, according to Zhou et al (2008), and taken as an index of germinating spore leakage. Negative control was similarly prepared, but without treatments.…”

Section: Methodsmentioning

confidence: 99%

Impeding <i>Bacillus</i> spore germination <i>in vitro</i> and in milk by soy glycinin during long cold storage

Mahgoub

Osman

Sitohy

2016

J. Gen. Appl. Microbiol.

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“…The release of UV-absorbing material concentrations were determined by UV spectrophotometer (Zhou et al, 2008). Loss of 260 nm absorbing material released from bacteria was measured by the technique performed by Devi et al (2010).Overnight broth cultures of E. coli ATCC 43889 in tryptone broth medium were adjusted to OD 600.…”

Section: Loss Of 260 Nm Absorbing Materialsmentioning

confidence: 99%

“…The measurement of release of UV-absorbing materials is an index of cell lysis (Zhou et al, 2008). The leakage of cytoplasmic membrane was analyzed by determining the release of cellular materials including nucleic acids, metabolites and ions, which were absorbed at 260 nm into the bacterial suspensions (Bajpai et al, 2014).…”

Section: Effect Of Nsaids On Leakage Of 260 Nm Absorbing Materials Frmentioning

confidence: 99%

Evaluation of antibacterial activity of some non-steroidal anti-inflammatory drugs against Escherichia coli causing urinary tract infection

Ahmed¹,

El-Baky²,

Ahmed³

et al. 2016

Afr. J. Microbiol. Res.

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Extensive use of antibiotics for urinary tract infections has led to the emergence of drug-resistant microorganisms and one solution to this problem is to search for non-antibiotic compounds that exert anti-bacterial activity through different mechanisms such as non-steroidal anti-inflammatory drugs (NSAIDs). In this study, out of 100 urine samples; 48 Escherichia coli strains were detected, 47.9% were multi-drug resistant. The antibiogram resistance pattern of the strains was carried out by agar dilution method. Diclofenac sodium, indomethacin, aspirin and ibuprofen were tested against the E. coli isolates. Diclofenac sodium showed the lowest MIC 50 and MIC 90 ; 8 and 256 g/ml, respectively. Aspirin showed MIC 50 of 64 g/ml, while both indomethacin and ibuprofen showed MIC 50 of 256 g/ml. Indomethacin, aspirin and ibuprofen showed the same MIC 90 of 1024 g/ml. The combined effects of the four NSAIDs and five antibiotics (Amoxicillin, Augmentin, Cefotaxime, Ciprofloxacin and Gentamicin) were tested on five resistant clinical E. coli strains by checkerboard dilution technique. All the tested NSAIDs significantly reduced the minimum inhibitory concentrations (MICs) of antibiotics against the tested bacteria and fractional inhibitory concentration indices (FICIs) for this combination ranged from 0.03 to 0.5. In this study, leakage of intracellular components suggests that the effect of NSAIDs on E. coli could be the formation of pores in the plasma membrane and scanning electron microscopy (SEM) observations confirmed the damage to the structural integrity of the tested bacteria. In conclusion, NSAIDs showed antibacterial activity against E. coli causing urinary tract infections (UTIs), a combination of them and antibiotics exhibited good synergism and the mechanism of their action was by damaging the bacterial cell membrane.

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Mode of action of pentocin 31-1: An antilisteria bacteriocin produced by Lactobacillus pentosus from Chinese traditional ham

Cited by 99 publications

References 25 publications

Characterization of Partially Purified Bacteriocin Like Substance (BLIS) Produced by Probiotic Lactobacillus Strains

Characterization of Partially Purified Bacteriocin Like Substance (BLIS) Produced by Probiotic Lactobacillus Strains

Impeding <i>Bacillus</i> spore germination <i>in vitro</i> and in milk by soy glycinin during long cold storage

Evaluation of antibacterial activity of some non-steroidal anti-inflammatory drugs against Escherichia coli causing urinary tract infection

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