Mode of action of fluopyram in plant-parasitic nematodes

Schleker, A Sylvia S; Rist, Marc; Matera, Christiane; Damijonaitis, Arunas; Collienne, Ursel; Matsuoka, Koichi; Habash, Samer S.; Twelker, Katja; Gutbrod, Oliver; Saalwächter, Corinna; Windau, Maren; Matthiesen, Svend; Stefanovska, Tatyana; Scharwey, Melanie; Marx, Michael Thomas; Geibel, Sven; Grundler, Florian M. W.

doi:10.1038/s41598-022-15782-7

Cited by 30 publications

(23 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relative amounts of ATP, adenosine diphosphate (ADP), and adenosine monophosphate (AMP) in organisms reveal the potential effects of pesticides on energy metabolism. Therefore, an adenylate energy charge (AEC) assay was performed to illustrate ATP biosynthesis according to the method described by Schleker et al 31 Nematodes were treated with the flu nanoagent (20 mg/L) or flu for 48 h, washed with PBS, and collected. Nucleotides were extracted and used to examine ATP biosynthesis using an ATP, ADP, AMP Content High-Performance Liquid Chromatography (HPLC) Assay Kit (Solarbio Science & Technology Co., Ltd).…”

Section: Particle Size Measurement and Morphologymentioning

confidence: 99%

“…Flu is a highly selective SDH inhibitor of nematodes and fungal pathogens that does not affect the activity of mammals, insects, or earthworms' SDH. 31 A recent publication reported that SDH activity was significantly reduced in nematodes treated with flu. 30 As shown in Figure 5B, SDH inhibition was consistently higher in flu nanoagent-exposed nematodes compared to flu alone at all tested concentrations, and the pIC 50 value was increased from 8.81 to 11.04 with the aid of SPc, revealing that SDH activity was further inhibited by SPc-delivered flu.…”

Section: Amplified Adverse Impacts Of Flu Nanoagents On Oxidative Str...mentioning

confidence: 99%

See 1 more Smart Citation

Self-Assembled Nanonematicide Induces Adverse Effects on Oxidative Stress, Succinate Dehydrogenase Activity, and ATP Generation

Peng

Jian

Long

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Long-term overuse of chemical nematicides has resulted in low control efficacy toward destructive root-knot nematodes, and continuous development in nanotechnology is supposed to enhance the utilization efficiency of nematicides to meet practical needs. Herein, a cationic star polymer (SPc) was constructed to load fluopyram (flu) and prepare a flu nanoagent. Hydrogen bonding and van der Waals forces facilitated the self-assembly of the flu nanoagent, leading to the breakdown of self-aggregated flu and reducing its particle size to 60 nm. The bioactivity of flu was remarkably improved, with the half lethal concentration 50 from 8.63 to 5.70 mg/L due to the help of SPc. Transcriptome analysis found that a large number of transport-related genes were upregulated in flu nanoagent-exposed nematodes, while the expression of many energy-related genes was disturbed, suggesting that the enhanced uptake of flu nanoagents by nematodes might lead to the disturbance of energy synthesis and metabolism. Subsequent experiments confirmed that exposure to flu nanoagents markedly increased the reactive oxygen species (ROS) level of nematodes. Compared to flu treatment alone, succinate dehydrogenase (SDH) activity was inhibited in flu nanoagent-exposed nematodes with an increase in the pIC50 from 8.81 to 11.04, which further interfered with adenosine triphosphate (ATP) biosynthesis. Furthermore, the persistence of SPc-loaded flu in soil was prolonged by 2.33 times at 50 days after application. The protective effects of flu nanoagents on eggplant seedlings were significantly improved in both greenhouse and field trials, and the root-knot number was consistently smaller in roots treated with flu nanoagents than in those treated with flu alone. Overall, this study successfully constructed a self-assembled flu nanoagent with amplified effects on oxidative stress, SDH activity, and ATP generation, leading to highly effective control of root-knot nematodes in the field.

show abstract

Section: Particle Size Measurement and Morphologymentioning

confidence: 99%

Section: Amplified Adverse Impacts Of Flu Nanoagents On Oxidative Str...mentioning

confidence: 99%

Self-Assembled Nanonematicide Induces Adverse Effects on Oxidative Stress, Succinate Dehydrogenase Activity, and ATP Generation

Peng

Jian

Long

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…This fungicide acts by inhibiting the succinate dehydrogenase enzyme in complex II of the electron transport chain of mitochondria. 1,2 Although the fluopyram molecule is already well known in several countries in Europe, the United States and also Australia, this compound was only authorized for marketing and use in Brazil in 2019.…”

Section: Introductionmentioning

confidence: 99%

Optimization and Validation of the Miniaturized Solid-Liquid Extraction with Low Temperature Purification (SLE-LTP) Method for Determining Fluopyram in Sandy, Clayey and Medium-Textured Soil

Silva¹,

Terra²,

Sicupira³

et al. 2023

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Fluopyram is a fungicide which can also be used as a nematicide in agricultural areas. The Brazilian Ministry of Agriculture authorized the commercialization and use of this molecule in Brazilian agriculture in 2019, but studies involving the development of an extraction and quantification method of this compound in environmental matrices such as soil are still scarce. Therefore, the present study aimed to optimize and validate the miniaturized version of the solid-liquid extraction with low temperature purification (SLE-LTP) method for determining this compound in sandy, clayey and medium-textured soil samples. All analyzes in this study were performed by gas chromatography coupled to mass spectrometry (GC-MS). The results revealed that the analyte recovery percentages in the three soil types ranged from 86 to 114% with a relative standard deviation of less than 15%. In addition to employing smaller amounts of reagents and sample, the miniaturized SLE-LTP method was selective, precise, exact, and linear in the range from 3 to 210 μg kg-1, and reached a limit of quantification lower than 3.00 μg kg-1 for the three soil types. The extraction method was applied to 30 real samples collected in coffee growing regions, but no residue of this compound was detected in these samples.

show abstract

“…Fluopyram and pydiflumetofen, introduced in the last decade, are systemic succinate dehydrogenase inhibitors (SDHI) fungicides, protecting soybean seedlings against soil-borne diseases, including SCN and other plant-parasitic nematodes. Similar to fungi, the succinate dehydrogenase (SDH) complex is also the target for fluopyram and pydiflumetofen in plant-parasitic nematodes ( Heiken, 2017 ; Schleker et al., 2022 ). In the mitochondrial matrix, SDH catalyzes the oxidation of succinate to fumarate, transferring electrons to ubiquinone without pumping protons across the mitochondrial inner membrane ( Huang and Millar, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Fluopyram activates systemic resistance in soybean

et al. 2022

View full text Add to dashboard Cite

The soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is a significant yield-limiting factor in soybean production in the Midwestern US. Several management practices are implemented to mitigate yield losses caused by SCN, including using SDHI (succinate dehydrogenase inhibitors) fungicides delivered as seed treatments. A set of studies was conducted to evaluate the effect of two seed-applied succinate dehydrogenase inhibitors (SDHI) compounds, fluopyram and pydiflumetofen, on SCN population densities, plant injury, and plant growth. Cyst counts in untreated control and pydiflumetofen treated plants were 3.44 and 3.59 times higher than fluopyram, respectively, while egg counts were 8.25 and 7.06 times higher in control and pydiflumetofen. Next-generation sequencing was later employed to identify transcriptomic shifts in gene expression profiles in fluopyram and pydiflumetofen -treated seedlings. RNA expression patterns of seed treatments clustered by sampling time (5 DAP vs. 10 DAP); therefore, downstream analysis was conducted by timepoint. At 5 DAP, 10,870 and 325 differentially expressed genes (DEG) were identified in fluopyram and pydiflumetofen, respectively. These same treatments generated 219 and 2 DEGs at 10 DAP. Multiple DEGs identified in soybean seedlings treated with fluopyram are linked to systemic resistance, suggesting a potential role of systemic resistance in the suppression of SCN by fluopyram, in addition to the known nematicidal activity. The non-target inhibition of soybean succinate dehydrogenase genes by fluopyram may be the origin of the phytotoxicity symptoms observed and potentially the source of the systemic resistance activation reported in the current study. This work helps to elucidate the mechanisms of suppression of SCN by fluopyram.

show abstract

Mode of action of fluopyram in plant-parasitic nematodes

Cited by 30 publications

References 23 publications

Self-Assembled Nanonematicide Induces Adverse Effects on Oxidative Stress, Succinate Dehydrogenase Activity, and ATP Generation

Self-Assembled Nanonematicide Induces Adverse Effects on Oxidative Stress, Succinate Dehydrogenase Activity, and ATP Generation

Optimization and Validation of the Miniaturized Solid-Liquid Extraction with Low Temperature Purification (SLE-LTP) Method for Determining Fluopyram in Sandy, Clayey and Medium-Textured Soil

Fluopyram activates systemic resistance in soybean

Contact Info

Product

Resources

About