Mode of Action of Disinfection Chemicals on the Bacterial Spore Structure and Their Raman Spectra

Malyshev, Dmitry; Dahlberg, Tobias; Wiklund, Krister; Andersson, Per Ola; Henriksson, Sara; Andersson, Magnus

doi:10.1021/acs.analchem.0c04519

Cited by 47 publications

(51 citation statements)

References 50 publications

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“…For example, by trapping oocysts in an LTRS instrument and exposing them to chemicals, one can measure changes and shifts of Raman peaks with time. These experiments can provide relevant information on the chemicals' mode of action on oocysts, similarly to what has been done on bacterial spores 32 . In addition, volume mapping using confocal Raman can provide more details of how sporozoites are organized inside the oocysts.…”

Section: Raman Spectra Of Viable and Neutralized C Parvummentioning

confidence: 90%

Reference Raman Spectrum and Mapping of Cryptosporidium parvum Oocysts

Malyshev

Dahlberg

Öberg

et al. 2022

Preprint

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Cryptosporidium parvum is a protozoan parasite and among the most infectious diarrhea-causing pathogens, leading to severe health problems for malnourished children and immunocompromised individuals. Outbreaks are common even in developed countries, originating from water or food contamination and resulting in suffering and large costs for society. Therefore, robust, fast and highly specific detection strategies of Cryptosporidium are needed. Label-free detection techniques such as Raman spectroscopy have been suggested, however high-resolution reported spectra in the literature are limited. In this work, we report reference Raman spectra at 3 cm^{-1} resolution for viable and inactivated Cryptosporidium oocysts of the species C. parvum, gathered at a single oocyst level using a laser tweezers Raman spectroscopy system. We furthermore provide tentative Raman peak assignments for the Cryptosporidium oocysts, along with Raman mapping of the oocysts' heterogeneous internal structure. Finally, we compare the C. parvum Raman spectrum with other common enterotoxigenic pathogens: Escherichia coli, Vibrio cholerae, Bacillus cereus and Clostridium difficile. Our results show a significant difference between C. parvum Raman spectra and the other pathogens.

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Section: Raman Spectra Of Viable and Neutralized C Parvummentioning

confidence: 90%

Reference Raman Spectrum and Mapping of Cryptosporidium parvum Oocysts

Malyshev

Dahlberg

Öberg

et al. 2022

Preprint

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“…To trap particles and acquire their Raman spectra, we use a custom-built LTRS instrument built around a modified inverted microscope (IX71, Olympus) previously described in [19][20][21]; see Fig. 1.…”

Section: Methodsmentioning

confidence: 99%

Optical design for laser tweezers Raman spectroscopy setups for increased sensitivity and flexible spatial detection

Dahlberg

Andersson

2021

Appl. Opt.

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We demonstrate a method to double the collection efficiency in laser tweezers Raman spectroscopy (LTRS) by collecting both the forward-scattered and backscattered light in a single-shot multitrack measurement. Our method can collect signals at different sample volumes, granting both the pinpoint spatial selectivity of confocal Raman spectroscopy and the bulk sensitivity of non-confocal Raman spectroscopy simultaneously. Further, we display that our approach allows for reduced detector integration time and laser power. To show this, we measure the Raman spectra of both polystyrene beads and bacterial spores. For spores, we can trap them at 2.5 mW laser power and acquire a high signal-to-noise ratio power spectrum of the calcium-dipicolinic acid peaks using an integration time of 2 × 30 s. Thus, our method will enable the monitoring of biological samples sensitive to high intensities for longer times. Additionally, we demonstrate that by a simple modification, we can add polarization sensitivity and retrieve extra biochemical information.

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“…An attempt to create a molecular basis for explaining the mechanism of the sporicidal action of ClO 2 has been described elsewhere [ 98 ]. The study focused on the observation of the expected changes in the chemical structure of DPA and DNA as well as selected amino acids of Bacillus thuringiensis spores exposed to the ClO 2 solution with a concentration of 750 mg/L.…”

Section: Characterization Of Biocides Against Clostridioides Difficile Used In Healthcare Settingsmentioning

confidence: 99%

Inactivation of Spores and Vegetative Forms of Clostridioides difficile by Chemical Biocides: Mechanisms of Biocidal Activity, Methods of Evaluation, and Environmental Aspects

Augustyn

Chruściel

Hreczuch

et al. 2022

IJERPH

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Clostridioides difficile infections (CDIs) are the most common cause of acquired diseases in hospitalized patients. Effective surface disinfection, focused on the inactivation of the spores of this pathogen, is a decisive factor in reducing the number of nosocomial cases of CDI infections. An efficient disinfection procedure is the result of both the properties of the biocidal agent used and the technology of its implementation as well as a reliable, experimental methodology for assessing the activity of the biocidal active substance based on laboratory models that adequately represent real clinical conditions. This study reviews the state of knowledge regarding the properties and biochemical basis of the action mechanisms of sporicidal substances, with emphasis on chlorine dioxide (ClO2). Among the analyzed biocides, in addition to ClO2, active chlorine, hydrogen peroxide, peracetic acid, and glutaraldehyde were characterized. Due to the relatively high sporicidal effectiveness and effective control of bacterial biofilm, as well as safety in a health and environmental context, the use of ClO2 is an attractive alternative in the control of nosocomial infections of CD etiology. In terms of the methods of assessing the biocidal effectiveness, suspension and carrier standards are discussed.

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Mode of Action of Disinfection Chemicals on the Bacterial Spore Structure and Their Raman Spectra

Cited by 47 publications

References 50 publications

Reference Raman Spectrum and Mapping of Cryptosporidium parvum Oocysts

Reference Raman Spectrum and Mapping of Cryptosporidium parvum Oocysts

Optical design for laser tweezers Raman spectroscopy setups for increased sensitivity and flexible spatial detection

Inactivation of Spores and Vegetative Forms of Clostridioides difficile by Chemical Biocides: Mechanisms of Biocidal Activity, Methods of Evaluation, and Environmental Aspects

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