Mode of Action of 1-Naphthylphthalamic Acid in Conspicuous Local Stem Swelling of Succulent Plant, Bryophyllum calycinum: Relevance to the Aspects of Its Histological Observation and Comprehensive Analyses of Plant Hormones

Marasek-Ciołakowska, Agnieszka; Dziurka, Michał; Kowalska, Urszula; Góraj-Koniarska, Justyna; Ueda, Junichi; Miyamoto, Kazuaki

doi:10.3390/ijms22063118

Cited by 4 publications

(4 citation statements)

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“…paste, where lanolin alone did not affect morphological changes in the internode segments. This situation was demonstrated in previous works [27,31,35]. In the stem of B. calycinum, IAA applied to a decapitated shoot or internode explants prevented the formation of the SAZ induced by JA-Me [31,33].…”

Section: Effect Of Ja-me On Gibberellinssupporting

confidence: 74%

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A Possible Mode of Action of Methyl Jasmonate to Induce the Secondary Abscission Zone in Stems of Bryophyllum calycinum: Relevance to Plant Hormone Dynamics

Dziurka

Góraj-Koniarska

Marasek-Ciołakowska

et al. 2022

Plants

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Plants can react to environmental stresses through the abscission of infected, damaged, or senescent organs. A possible mode of action of methyl jasmonate (JA-Me) to induce the formation of the secondary abscission zone (SAZ) in the stems of Bryophyllum calycinum was investigated concerning plant hormone dynamics. Internode segments were prepared mainly from the second or third internode from the top of plants with active elongation. JA-Me applied to the middle of internode segments induced the SAZ formation above and below the treatment after 5–7 days. At 6 to 7 days after JA-Me treatment, the above and below internode pieces adjacent to the SAZ were excised and subjected to comprehensive analyses of plant hormones. The endogenous levels of auxin-related compounds between both sides adjacent to the SAZ were quite different. No differences were observed in the level of jasmonic acid (JA), but the contents of 12-oxo-phytodienoic acid (OPDA), a precursor of JA, and N-jasmonyl-leucine (JA-Leu) substantially decreased on the JA-Me side. Almost no effects of JA-Me on the dynamics of other plant hormones (cytokinins, abscisic acid, and gibberellins) were observed. Similar JA-Me effects on plant hormones and morphology were observed in the last internode of the decapitated growing plants. These suggest that the application of JA-Me induces the SAZ in the internode of B. calycinum by affecting endogenous levels of auxin- and jasmonate-related compounds.

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Section: Effect Of Ja-me On Gibberellinssupporting

confidence: 74%

“…Analyses of plant hormones were performed according to the methods reported previously [27,35,[54][55][56]. At 6 to 7 days after treatment with JA-Me, the below (nonsenescent, green) and above (senescent, yellow) parts of ca.…”

Section: Analyses Of Plant Hormones In Relation To the Formation Of The Secondary Abscission Zone Induced By Methyl Jasmonatementioning

confidence: 99%

A Possible Mode of Action of Methyl Jasmonate to Induce the Secondary Abscission Zone in Stems of Bryophyllum calycinum: Relevance to Plant Hormone Dynamics

Dziurka

Góraj-Koniarska

Marasek-Ciołakowska

et al. 2022

Plants

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“…The accumulated endogenous auxin in inflorescence stems has been considered to be important for the hypergravity-enhanced expression of genes related to lignin biosynthesis. Marasek-Ciolakowska et al (2021) showed that the application of 1-naphtylphtalamic acid, an inhibitor of polar auxin transport, induced conspicuous local stem swelling in the area of its application and significantly increased endogenous IAA level in the area of stem swelling in Bryophyllum calycinum. In etiolated pea seedlings hypergravity conditions substantially enhanced stem swelling and reduced elongation growth of the epicotyls (Fig.…”

Section: Discussionmentioning

confidence: 99%

Hypergravity conditions inhibit elongation growth and polar auxin transport in epicotyls of etiolated pea seedlings

2023

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Growth and development, and polar auxin transport in epicotyls of etiolated pea (Pisum sativum L. cv Alaska) seedlings grown under hypergravity conditions produced by centrifugation were studied. Seeds were allowed to germinate and grow under various magnitude of hypergravity conditions for 3.5 days in the dark. Hypergravity at up to 50 G had little effect on epicotyl elongation growth, but increasing gravity force up to 300 G resulted in a reduction of elongation growth, the reduction at 100 G and 200 G being ca. 30% and 50% of control, respectively. Although expressions of genes playing an important role in polar auxin transport, auxin efflux carrier PsPINs and influx carrier PsAUX1, were not affected by hypergravity conditions, polar auxin transport activities of the epicotyls were substantially reduced, those at 100 G and 200 G being ca. 80% and 60% of control, respectively. In addition, close relationships between inhibition of elongation growth and polar auxin transport of the epicotyls were observed. These results suggest that hypergravity-regulated polar auxin activity is involved in inhibition of elongation growth of epicotyls of etiolated pea seedlings. The mechanisms to regulate elongation growth and polar auxin transport are discussed.

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“…The concentrations of endogenous hormones, including IAA, ABA, GA, and zeatin (ZT) in leaves and roots were determined according to the methods previously reported by Marasek-Ciolakowska et al (2021) . Frozen leaves or roots (2.5 g) were ground with a mortar and pestle to a powder in liquid nitrogen, then the powder was rapidly transferred to a 50 mL centrifuge tube and extracted with 20 mL of 80% methanol at 4 °C overnight.…”

Section: Methodsmentioning

confidence: 99%

Effects of plant age on antioxidant activity and endogenous hormones in Alpine Elymus sibiricus of the Tibetan Plateau

Liu

et al. 2023

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Elymus sibiricus L. is a perennial forage species that has potential to serve as a forage source in livestock grazing systems. However, E. sibiricus has been shown to have a rapid and substantial reduction of aboveground biomass and seed yield after 3 or 4 years and an accelerated aging process. To determine possible aging mechanisms, we planted E. sibiricus seeds in triplicate blocks in 2012, 2015, and 2016, respectively, and harvested samples of leaves and roots at the jointing and heading stages in 2018 and 2019 to determine oxidative indices and endogenous hormones. The fresh aboveground biomass of 4- and 5-year old plants declined by 34.2% and 52.4% respectively compared with 3-year old plants, and the seed yield declined by 12.7% and 34.1%, respectively. The water content in leaves was 51.7%, 43.3%, and 35.6%, and net photosynthesis was 7.73, 6.35, and 2.08 µmol/m2·s in 3-, 4-, and 5-year old plants, respectively. The superoxide anion radical generation rate in leaves and roots did not show any aging pattern. There was a non-significant increase in malondialdehyde concentration with plant age, particularly in leaves and roots at the heading stage in 2019. The superoxide dismutase activity showed a declining trend with age of plant roots at the jointing stage in both 2018 and 2019. The peroxidase activity declined with plant age in both leaves and roots, for example, and the catalase activity in roots 4- and 7-year old plants declined by 13.8% and 0.85%, respectively, compared to 3-year old plants at the heading stage in 2018. Therefore, the reduced capacity of the antioxidant system may lead to oxidative stress during plant aging process. Overall, the concentrations of plant hormones, auxin (IAA), gibberellin (GA), zeatin (ZT), and abscisic acid (ABA) were significantly lower in roots than in leaves. The IAA concentration in leaves and roots exhibited different patterns with plant age. The ZT concentrations in leaves of 3-year old plants was 2.39- and 2.62-fold of those in 4- and 7-year old plants, respectively at the jointing stage, and in roots, the concentration declined with plant age. The changes in the GA concentration with plant age varied between the physiological stages and between years. The ABA concentrations appeared to increase with plant age, particularly in leaves. In conclusion, the aging process of E. sibiricus was apparently associated with an increase in oxidative stress, a decrease of ZT and an increase of ABA, particularly in roots. These findings highlight the effects of plant age on the antioxidant and endogenous hormone activity of E. sibiricus. However, these plant age-related trends showed variations between plant physiological stages and between harvest years that needs to be researched in the future to develop strategies to manage this forage species.

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Mode of Action of 1-Naphthylphthalamic Acid in Conspicuous Local Stem Swelling of Succulent Plant, Bryophyllum calycinum: Relevance to the Aspects of Its Histological Observation and Comprehensive Analyses of Plant Hormones

Cited by 4 publications

References 45 publications

A Possible Mode of Action of Methyl Jasmonate to Induce the Secondary Abscission Zone in Stems of Bryophyllum calycinum: Relevance to Plant Hormone Dynamics

A Possible Mode of Action of Methyl Jasmonate to Induce the Secondary Abscission Zone in Stems of Bryophyllum calycinum: Relevance to Plant Hormone Dynamics

Hypergravity conditions inhibit elongation growth and polar auxin transport in epicotyls of etiolated pea seedlings

Effects of plant age on antioxidant activity and endogenous hormones in Alpine Elymus sibiricus of the Tibetan Plateau

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